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proMpEF:YC3.60/pMpGWB103 (#RDB20983)

Expression vector of Yellow Cameleon 3.60 (YC3.60) under the control of the MpEF promoter in Marchantia polymorpha. YC3.60 coding sequence was codon optimized for Arabidopsis.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Iwano, M., Plant Physiol. 197 (1): kiae613 (2024). PMID 39535860. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of Yellow Cameleon 3.60 (YC3.60) under the control of the MpEF promoter in Marchantia polymorpha. YC3.60 coding sequence was codon optimized for Arabidopsis. See Fig.3 of Iwano, M. et al., Plant Physiol., 197(1):kiae613, 2024. doi: 10.1093/plphys/kiae613
Comment YC3.60 is a chimeric protein consisting of cyan fluorescent protein (CFP), calmodulin (CaM), a glycylglycine linker, the CaM-binding domain of myosin light-chain kinase (M13), and the circularly permuted Venus protein (cpVenus).
Vector backbone pMpGWB103 (plasmid)
Selectable markers Spectinomycin
Growth conditions LB+Spectinomycin 100 μg/mL 37℃
Gene/insert name Marchantia polymorpha MpEF promoter genomic DNA; Xenopus laevis calmodulin (CaM) cDNA; synthetic myosin light-chain kinase (M13) cDNA; Aequorea victoria fluorescent protein (CFP, cpVenus).
Depositor|Developer Iwano, Megumi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Megumi Iwano et al. Plant Physiology, Volume 197, Issue 1, 2025, kiae613) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Kyoto University.
Additional terms and conditions:
Regarding Yellow cameleon 3.60
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Proc. Natl. Acad. Sci. U S A. 101:10554-10559 (2004).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Megumi Iwano et al. Plant Physiology, Volume 197, Issue 1, 2025, kiae613)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、京都大学の許可を得ること。
付加的使用条件：
Yellow cameleon 3.60 について
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Proc. Natl. Acad. Sci. U S A. 101:10554-10559 (2004).

Catalog # Resource name Shipping form Fee
RDB20983 proMpEF:YC3.60/pMpGWB103 DNA solution

How to cite this biological resource

Materials & Methods section:

The proMpEF:YC3.60/pMpGWB103 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20983).

Reference section:

Iwano, M., Suetsugu, N., Nishihama, R., Ishida, S., Horie, T., Costa, A., Katsuno, T., Kimura, M., Iida, K., Iida, H., Nagai, T., Kohchi, T., MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha. Plant Physiol. 197 (1): kiae613 (2024). PMID 39535860. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Iwano, M., MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha. Plant Physiol. 197 (1): kiae613 (2024). PMID 39535860. [PubMed] [Article] [RRC of NBRP]