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pcDNA3-GeuSapVC3.60 (#RDB20915)

Mamalian expression vector of GeudaSapphire-Venus-Cameleon 3.60, a Ca2+ FADED sensor.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Laskaratou, D., Nat. Commun. 12 (1): 2541 (2021). PMID 33953187. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name GeuSapVC3.60
Clone info. Mamalian expression vector of GeudaSapphire-Venus-Cameleon 3.60, a Ca2+ FADED sensor.
Vector backbone pcDNA3 (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name Aequorea victoria GFP (GeudaSapphire and Venus); X. laevis CaM cDNA; synthetic myosin light chain kinase peptide (M13).
Depositor|Developer Mizuno, Hideaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Laskaratou, D., et al. Nat. Commun. 12, 2541, 2021) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the KU Leuven.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Proc. Natl. Acad. Sci. U S A. 101:10554-10559 (2004).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Laskaratou, D., et al. Nat. Commun. 12, 2541, 2021) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the KU Leuven.
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Proc. Natl. Acad. Sci. U S A. 101:10554-10559 (2004).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee
RDB20915 pcDNA3-GeuSapVC3.60 DNA solution

How to cite this biological resource

Materials & Methods section:

The pcDNA3-GeuSapVC3.60 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20915).

Reference section:

Laskaratou, D., Fernández, G.S., Coucke, Q., Fron, E., Rocha, S., Hofkens, J., Hendrix, J., Mizuno, H., Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor. Nat. Commun. 12 (1): 2541 (2021). PMID 33953187. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Laskaratou, D., Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor. Nat. Commun. 12 (1): 2541 (2021). PMID 33953187. [PubMed] [Article] [RRC of NBRP]