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pEF-TIR1(F74G)-P2A-AID-2xRBD27 (#RDB20908)

Expression vector of OsTIR-L1 mutant (F74G) and tandem RBD27 tagged with AID at N-terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
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Publication Nishimura, K., Nat Methods 6(12), 917-922, (2009). PMID 19915560. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of Oryza sativa F-box transport inhibitor response 1 (TIR-L1) mutant (F74G) and tandem RBD27 (mouse Slp2-a-SHD + rat Slp2-a-SHD) tagged with auxin-induced degron (AID) at N-terminus.
Comment Originated from pMRX-bsr-Myc-OsTIR1(F74G) (RDB20713) for F74G and pMRX-bsr-Flag-AID-Rab5A (RDB19430) for AID.
Vector backbone pEF-BOS (plasmid)
Growth conditions LB+KM, 37oC
Gene/insert name Oryza sativa TIR-L1 (F74G mutant); mouse Sytl2 cDNA; rat Sytl2 cDNA; Arabidopsis thaliana IAA17 (AID) cDNA.
Depositor|Developer Fukuda, Mitsunori |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Nishimura, K. et al. Nat. Methods 6, 917–922, 2009) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Tohoku University.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nat. Methods 6, 917–922, 2009)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、東北大学の許可を得ること。

Catalog # Resource name Shipping form Fee
RDB20908 pEF-TIR1(F74G)-P2A-AID-2xRBD27 DNA solution

How to cite this biological resource

Materials & Methods section:

The pEF-TIR1(F74G)-P2A-AID-2xRBD27 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20908).

Reference section:

Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., Kanemaki, M., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods 6(12), 917-922, (2009). PMID 19915560. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods 6(12), 917-922, (2009). PMID 19915560. [PubMed] [Article] [RRC of NBRP]