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pEGFP-C1-2xRBD27 (#RDB20906)

Expression vector of tandem RBD27 with EGFP at N-terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sugawara, A., J. Cell Sci. jcs.263878 (2025). PMID 40351074. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of tandem RBD27 (mouse Slp2-a-SHD + rat Slp2-a-SHD) with EGFP at N-terminus; SHD, synaptotagmin-like protein homology domain.
Vector backbone pEGFP-C1 (plasmid)
Growth conditions LB+KM, 37oC
Gene/insert name mouse Sytl2 cDNA; rat Sytl2 cDNA.
Depositor|Developer Fukuda, Mitsunori |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Sugawata, A., Maruta, Y. and Fukuda, M. J. Cell Sci., under revision, 2025) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Tohoku University.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sugawata, A., Maruta, Y. and Fukuda, M. J. Cell Sci., under revision, 2025)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、東北大学の許可を得ること。

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RDB20906 pEGFP-C1-2xRBD27 DNA solution

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-2xRBD27 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20906).

Reference section:

Sugawara, A., Maruta, Y., Fukuda, M., AID-2×RBD27, an auxin-inducible degron-based Rab27 trapper that reversibly inhibits the function of Rab27A in melanocytes. J. Cell Sci. jcs.263878 (2025). PMID 40351074. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sugawara, A., AID-2×RBD27, an auxin-inducible degron-based Rab27 trapper that reversibly inhibits the function of Rab27A in melanocytes. J. Cell Sci. jcs.263878 (2025). PMID 40351074. [PubMed] [Article] [RRC of NBRP]