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pCMV5-Flag-Sin1 (#RDB20790)

Mamalian expression vector of human Sin1 (MAPKAP1) Flag-tag at N-terminus.

Clone info. Mamalian expression vector of human Sin1 (MAPKAP1) Flag-tag at N-terminus. See Fig.2 of Tsutsumi, K. et al., Sci. Rep., 13 (1): 20956, 2023 (doi: 10.1038/s41598-023-47892-1).
Vector backbone pCMV5-Flag (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Gene/insert name human MAPKAP1 cDNA.
Depositor|Developer Tsutsumi, Koji |
 
Sequence (full) RDB20790hts01.seq checkAssembled from experimentally sequenced data.
Publication Tsutsumi, K., Sci. Rep. 13 (1): 20956 (2023). PMID 38065968. [link to RRC of NBRP]
Test sheet RDB20790C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Koji Tsutsumi et al. Sci Re. 13(1):20956, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Kitasato University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Koji Tsutsumi et al. Sci Re. 13(1):20956, 2023) 。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、北里大学の許可を得ること。　

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20790 pCMV5-Flag-Sin1 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCMV5-Flag-Sin1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20790).

Reference section:

Tsutsumi, K., Nohara, A., Tanaka, T., Murano, M., Miyagaki, Y., Ohta, Y., FilGAP regulates tumor growth in Glioma through the regulation of mTORC1 and mTORC2. Sci. Rep. 13 (1): 20956 (2023). PMID 38065968. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tsutsumi, K., FilGAP regulates tumor growth in Glioma through the regulation of mTORC1 and mTORC2. Sci. Rep. 13 (1): 20956 (2023). PMID 38065968. [link to RRC of NBRP]