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pMRX-bsr-Myc-OsTIR1(F74G) (#RDB20713)

Retroviral expression vector of oryza sativa F-box transport inhibitor response 1 (TIR-L1) mutant (F74G) tagged with Myc at N-terminus.

Clone info. Retroviral expression vector of oryza sativa F-box transport inhibitor response 1 (TIR-L1) mutant (F74G) tagged with Myc at N-terminus.
Vector backbone pMRX-IRES-bsr-FLAG (plasmid)
Growth conditions LB+Amp, 37oC
Gene/insert name oryza sativa TIR-L1 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB20713hts01.seq checkAssembled from experimentally sequenced data.
Publication Haga, K., Autophagy 21 (1): 21-36 (2025). PMID 38953305. [link to Journal]
Test sheet RDB20713C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Haga, K. and Fukuda, M. Autophagy 21, 21–36, 2025) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Tohoku University.
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Haga, K. and Fukuda, M. Autophagy 21, 21–36, 2025)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、東北大学の許可を得ること。
付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB20713 pMRX-bsr-Myc-OsTIR1(F74G) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMRX-bsr-Myc-OsTIR1(F74G) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20713).

Reference section:

Haga, K., Fukuda, M., Comprehensive knockout analysis of the RAB family small GTPases reveals an overlapping role of RAB2 and RAB14 in autophagosome maturation. Autophagy 21 (1): 21-36 (2025). PMID 38953305. [link to Journal]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Haga, K., Comprehensive knockout analysis of the RAB family small GTPases reveals an overlapping role of RAB2 and RAB14 in autophagosome maturation. Autophagy 21 (1): 21-36 (2025). PMID 38953305. [link to Journal]
reference Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods 6(12), 917-922, (2009). PMID 19915560. [link to RRC of NBRP]