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pcDNA3.1-Eys-c8805a (#RDB20446)

Expression vector of human c.8805-truncated EYS cDNA. CMV promoter, bGH polyA.

Clone info. Expression vector of human c.8805-truncated EYS cDNA. CMV promoter, bGH polyA. The c.8805C>A is a nonsense mutation located in the last EGF domain near the C-terminus, see SFig. 1C of Otsuka, Y. et al. JCI insight, 2024;9 (8): e174179, 2024 ( DOI: 10.1172/jci.insight.174179).
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name human EYS cDNA.
Depositor|Developer Inoue, Haruhisa |
 
Sequence (full) RDB20446hts01.seq checkAssembled from experimentally sequenced data.
Publication Otsuka, Y., JCI Insight. 9 (8): e174179 (2024). [link to RRC of NBRP]
Test sheet RDB20446C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Yuki Otsuka, JCI insight, 9(8):e17417920,2024)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yuki Otsuka, JCI insight, 9(8):e17417920,2024)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20446 pcDNA3.1-Eys-c8805a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-Eys-c8805a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20446).

Reference section:

Otsuka, Y., Imamura, K., Oishi, A., Asakawa, K., Kondo, T., Nakai, R., Suga, M., Inoue, I., Sagara, Y., Tsukita, K., Teranaka, K., Nishimura, Y., Watanabe, A., Umeyama, K., Okushima, N., Mitani, K., Nagashima, H., Kawakami, K., Muguruma, K., Tsujikawa, A., Inoue, H., Phototoxicity avoidance is a potential therapeutic approach for retinal dystrophy caused by EYS dysfunction. JCI Insight. 9 (8): e174179 (2024). PMID 38646933. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Otsuka, Y., Phototoxicity avoidance is a potential therapeutic approach for retinal dystrophy caused by EYS dysfunction. JCI Insight. 9 (8): e174179 (2024). PMID 38646933. [link to RRC of NBRP]