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pcDNA3.1-SphK2-V5-6His (#RDB20442)

Expression vector of human sphingosine kinase 2 (SphK2). V5His6-tag at C-terminus.

Clone info. Expression vector of human sphingosine kinase 2 (SphK2). V5His6-tag at C-terminus, Komai, M. et al., J. Lipid Res., 65 (3):100510, 2024 (DOI 10.1016/j.jlr.2024.100510).
Vector backbone pcDNA3.1V5His6 (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human SPHK2 cDNA; synthetic His-tag.
Depositor|Developer Takasugi, Nobumasa |
 
Sequence (full) RDB20442hts01.seq checkAssembled from experimentally sequenced data.
Publication Komai, M., J. Lipid Res. 65 (3): 100510 (2024). [link to RRC of NBRP]
Test sheet RDB20442C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Masato K. et al., J. Lipid Res. 65(3):100510, 2024) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the okayama University.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Masato K. et al., J. Lipid Res. 65(3):100510, 2024)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、岡山大学の許可を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20442 pcDNA3.1-SphK2-V5-6His DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-SphK2-V5-6His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20442).

Reference section:

Komai, M., Noda, Y., Ikeda, A., Kaneshiro, N., Kamikubo, Y., Sakurai, T., Uehara, T., Takasugi, N., Nuclear SphK2/S1P signaling is a key regulator of ApoE production and Aβ uptake in astrocytes. J. Lipid Res. 65 (3): 100510 (2024). PMID 38280459. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Komai, M., Nuclear SphK2/S1P signaling is a key regulator of ApoE production and Aβ uptake in astrocytes. J. Lipid Res. 65 (3): 100510 (2024). PMID 38280459. [link to RRC of NBRP]