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pCaMKII-iBB-GECO1 (#RDB20304)

Expression vector of iBB-GECO1 (mIFP-Calmodulin-RS20-mIFP).

Clone info. Expression vector of iBB-GECO1 (mIFP-Calmodulin-RS20-mIFP). iBB-GECO1 is a genetically encoded far-red fluorescent calcium ion biosensor derived from a biliverdin-binding protein. For more information, see Hashizume, R. et al., Protein Sci., 31 (10): e4440, 2022 (DOI: 10.1002/pro.4440).
Vector backbone pCaMKII-C1 (plasmid)
Gene/insert name Bradyrhizobium sp. mIFP; Chicken smooth muscle myosin light chain kinase (CaM-binding peptide, RS20); Rat Calmodulin.
Depositor|Developer Bito, Haruhiko |
 
Sequence (full) RDB20304hts01.seq checkAssembled from experimentally sequenced data.
Publication Hashizume, R., Protein Sci. 31 (10): e4440 (2022). [link to RRC of NBRP]
Test sheet RDB20304C0p1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Hashizume, R. et al. Protein Sci. 31 (10): e4440, 2022); (Dan Yu et al. Nat. Methods, 12 (8): 763-765, 2015) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20304 pCaMKII-iBB-GECO1 DNA solution

How to cite this biological resource

Materials & Methods section:

The pCaMKII-iBB-GECO1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20304).

Reference section:

Hashizume, R., Fujii, H., Mehta, S., Ota, K., Qian, Y., Zhu, W., Drobizhev, M., Nasu, Y., Zhang, J., Bito, H., Campbell, R.E., A genetically encoded far-red fluorescent calcium ion biosensor derived from a biliverdin-binding protein. Protein Sci. 31 (10): e4440 (2022). PMID 36173169. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hashizume, R., A genetically encoded far-red fluorescent calcium ion biosensor derived from a biliverdin-binding protein. Protein Sci. 31 (10): e4440 (2022). PMID 36173169. [link to RRC of NBRP]
reference Yu, D., A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat. Methods 12 (8): 763-765 (2015). PMID 26098020.