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pEF-Tyr-EGFP-FM4 (#RDB20230)

Expression vector of mouse tyrosinase. EGFP tag and FM4 (mouse FKBP12-derived oligomerization domain) tag at C-terminus.

Clone info. Expression vector of mouse tyrosinase. EGFP tag and FM4 (mouse FKBP12-derived oligomerization domain) tag at C-terminus. See Fig. 1A of Nakamura, H. and Fukuda, M., Sci. Rep. 14 (1): 2529 (2024).
Vector backbone pEF-BOS (plasmid)
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Tyr cDNA; Aequorea victoria GFP (EGFP); mouse Fkbp1a (FM4) cDNA.
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB20230hts01.seq checkAssembled from experimentally sequenced data.
Publication Nakamura, H., Sci. Rep. 14 (1): 2529 (2024). [link to RRC of NBRP]
Test sheet RDB20230C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Nakamura, H. & Fukuda, M. Sci. Rep. 14 (1): 2529 (2024) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Tohoku University.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakamura, H. & Fukuda, M. Sci. Rep. 14 (1): 2529 (2024)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、東北大学の許可を得ること。
付加的使用条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20230 pEF-Tyr-EGFP-FM4 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pEF-Tyr-EGFP-FM4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20230).

Reference section:

Nakamura, H., Fukuda, M., Establishment of a synchronized tyrosinase transport system revealed a role of Tyrp1 in efficient melanogenesis by promoting tyrosinase targeting to melanosomes. Sci. Rep. 14 (1): 2529 (2024). PMID 38291221. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakamura, H., Establishment of a synchronized tyrosinase transport system revealed a role of Tyrp1 in efficient melanogenesis by promoting tyrosinase targeting to melanosomes. Sci. Rep. 14 (1): 2529 (2024). PMID 38291221. [link to RRC of NBRP]