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pCAG/ATP9B(E272Q)-HA (#RDB20167)

Expression vector of human ATP9B ATPase-deficient mutant (E272Q) for mammalian cells, C-terminal HA-tag.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Tone, T., FEBS Lett. 594 (3): 412-423 (2020). PMID 31571211. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human ATP9B ATPase-deficient mutant (E272Q) for mammalian cells, C-terminal HA-tag.
Vector backbone pCAG (plasmid)
Growth conditions LB+Amp, 37℃
Gene/insert name human ATP9B cDNA; HA-tag.
Depositor|Developer Shin, Hye-Won |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tone, T. et al., FEBS Lett. 594(3): 412-423, 2020) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tone, T. et al., FEBS Lett. 594(3): 412-423, 2020) 。非営利学術機関の学術研究に限る。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee
RDB20167 pCAG/ATP9B(E272Q)-HA DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAG/ATP9B(E272Q)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20167).

Reference section:

Tone, T., Nakayama, K., Takatsu, H., Shin, H.W., ATPase reaction cycle of P4-ATPases affects their transport from the endoplasmic reticulum. FEBS Lett. 594 (3): 412-423 (2020). PMID 31571211. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tone, T., ATPase reaction cycle of P4-ATPases affects their transport from the endoplasmic reticulum. FEBS Lett. 594 (3): 412-423 (2020). PMID 31571211. [PubMed] [Article] [RRC of NBRP]