Resource data sheet
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pCSIIhyg-RGECO1.0

Lentivirus vector of calcium biosensor R-GECO1.0. with blasticidine resistance gene.

Catalog number RDB19907
Resource name pCSIIhyg-RGECO1.0
Clone info. Lentivirus vector of calcium biosensor, R-GECO1.0 with blasticidine resistance gene. R-GECO is a red-fluorescent calcium Indicator. Refer Zhao, Y., et al., Science, 333 (6051): 1888-1891 (2011).
Vector backbone pCSIIhyg (plasmid)
Selectable markers Ampicillin (E. coli), hygromycin (mammalian cell). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Chickin smooth muscle myosin light chain kinase cDNA; rat Calm2 cDNA; Discosoma sp. RFP (mApple).
Depositor|Developer Matsuda, Michiyuki | Hirota, Keisho |
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External Database
Chickin smooth muscle myosin light chain kinase rat Calm2 Discosoma sp. RFP
        Reference sequence
          
         
        Sequence (full) RDB19907hts01.seqcheck assembled from experimentally sequenced data
        Test sheet RDB19907_B3C6p1-1.pdf 

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.((Hirota, K et al. Endocrinology. 163(3): bqac005, 2022) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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        Please visit Ordering instruction.[link] 
        Remarks Remember that you will be working with samples containing infectious virus.
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirota, K et al. Endocrinology. 163(3): bqac005, 2022)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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        提供依頼書 [Word]
        専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。
        遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
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        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB19907 pCSIIhyg-RGECO1.0 DNA solution

        Ordering instruction of plasmids [in Japanese] [in English]

        How to cite this biological resource

        Materials & Methods section:

        The pCSIIhyg-RGECO1.0 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19907).

        Reference section:

        Hirota, K., Hirashima, T., Horikawa, K., Yasoda, A., Matsuda, M., C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. Endocrinology 163 (3): bqac005 (2022). PMID 35041746. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)

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        Featured content Lentiviral plasmid list (English text)

        References

        Original, user report and related articles

        original Hirota, K., C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. Endocrinology 163 (3): bqac005 (2022). PMID 35041746. [link to RRC of NBRP]

        2023.10.04

        GNP_filter3_RDBDEP_html_231001.pl