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pCSIIhyg-RGECO1.0 (#RDB19907)

Lentivirus vector of calcium biosensor R-GECO1.0. with blasticidine resistance gene.


Drawn by SnapGene® software
Sequence (full) RDB19907hts01.seq
Publication Hirota, K., Endocrinology 163 (3): bqac005 (2022). [link to RRC of NBRP]
Test sheet RDB19907_B3C6p1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Lentivirus vector of calcium biosensor, R-GECO1.0 with blasticidine resistance gene. R-GECO is a red-fluorescent calcium Indicator. Refer Zhao, Y., et al., Science, 333 (6051): 1888-1891 (2011).
Vector backbone pCSIIhyg (plasmid)
Selectable markers Ampicillin (E. coli), hygromycin (mammalian cell). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Chickin smooth muscle myosin light chain kinase cDNA; rat Calm2 cDNA; Discosoma sp. RFP (mApple).
Depositor|Developer Matsuda, Michiyuki | Hirota, Keisho |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.((Hirota, K et al. Endocrinology. 163(3): bqac005, 2022) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Remarks Remember that you will be working with samples containing infectious virus.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirota, K et al. Endocrinology. 163(3): bqac005, 2022)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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RDB19907 pCSIIhyg-RGECO1.0 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The pCSIIhyg-RGECO1.0 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19907).

Reference section:

Hirota, K., Hirashima, T., Horikawa, K., Yasoda, A., Matsuda, M., C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. Endocrinology 163 (3): bqac005 (2022). PMID 35041746. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hirota, K., C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. Endocrinology 163 (3): bqac005 (2022). PMID 35041746. [link to RRC of NBRP]