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pBF101

Expression vector of BSD (blasticidin-S deaminase) in a filamentous fungus, such as Pyricularia oryzae.

Catalog number RDB19905
Resource name pBF101
Clone info. BSD (blasticidin-S deaminase) is a seletion marker for prokaryotes and eukaryotes. pBF101 is a vector for expression of BSD in a filamentous fungus, such as Pyricularia oryzae.
Vector backbone pBluescript SK+ (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name Aspergillus nidulans trpC promoter, terminator; Aspergillus terreus BSD.
Reference of insert sequence D83710 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Kimura, Makoto | Yamaguchi, Isamu |
 
Sequence (full) RDB19905hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19905_B3Bsp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Kimura, M. et al., Mol. Gen. Genet. 242: 121-129, 1994)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kimura, M. et al., Mol. Gen. Genet. 242: 121-129, 1994)。
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RDB19905 pBF101 DNA solution


How to cite this biological resource

Materials & Methods section:

The pBF101 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19905).

Reference section:

Kimura, M., Kamakura, T., Tao, Q.Z., Kaneko, I., Yamaguchi, I., Cloning of the blasticidin S deaminase gene (BSD) from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae. Mol. Gen. Genet. 242 (2): 121-129 (1994). PMID 8159161. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Kimura, M., Cloning of the blasticidin S deaminase gene (BSD) from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae. Mol. Gen. Genet. 242 (2): 121-129 (1994). PMID 8159161. [link to RRC of NBRP]