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pMAMtracker-Luc (#RDB19895)

A reporter plasmid based on split-Luciferase for evaluating intracellular organelle (mitochondria-associated membrane).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sakai, S., FASEB J. 35 (7): e21688 (2021). PMID 34143516. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. A reporter plasmid based on split-Luciferase for evaluating intracellular organelle (mitochondria-associated membrane). See Fig.1 of Sakai, S. et al., FASEB J. 35 (7): e21688, 2021.
Comment Expression was confirmed by the depositor with western blotting, immunocytochemistry.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Kanamycin, 37oC
Gene/insert name human TOM20 cDNA, Oplophorus gracilirostris luciferase (SmBiT, LgBiT), S. cerevisiae UBC6 (ER anchor).
Depositor|Developer Yamanaka, Koji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain approval from the DEPOSITOR using the Approval Form. The availability of the BIOLOGICAL RESOURCE is restricted to a RECIPIENT of a not-for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Sakai S. et al., FASEB J. 35(7):e21688, 2021, doi: 10.1096/fj.202100137R). The Recipient acknowledges and agrees the Limited Use Label License of Promega Corporation for the use of SmBiT vector DNA incorporated in the BIOLOGICAL RESOURCE. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Remarks
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。利用者は、非営利機関の学術研究に限る。利用者は、研究成果の公表にあたって開発者の指定する文献を引用する(Sakai S. et al., FASEB J. 35(7):e21688. 2021, doi: 10.1096/fj.202100137R)。利用者はプロメガ社が示すLimited Use Label Licenseに従って本件リソースに含まれるSmBiT vector DNAを取り扱う。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
備考
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee
RDB19895 pMAMtracker-Luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMAMtracker-Luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19895).

Reference section:

Sakai, S., Watanabe, S., Komine, O., Sobue, A., Yamanaka, K., Novel reporters of mitochondria-associated membranes (MAM), MAMtrackers, demonstrate MAM disruption as a common pathological feature in amyotrophic lateral sclerosis. FASEB J. 35 (7): e21688 (2021). PMID 34143516. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sakai, S., Novel reporters of mitochondria-associated membranes (MAM), MAMtrackers, demonstrate MAM disruption as a common pathological feature in amyotrophic lateral sclerosis. FASEB J. 35 (7): e21688 (2021). PMID 34143516. [PubMed] [Article] [RRC of NBRP]