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Yellow Cameleon-Nano65/pcDNA3

Vector of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM, for mammalian expression.

Catalog number RDB19877
Resource name Yellow Cameleon-Nano65/pcDNA3
Clone info. Vector of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM, for mammalian expression. For more information, see Fig.1 of Horikawa, K. et al., Nat. Methods, 7 (9): 729-732, 2010.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Aequorea victoria GFP (CFP), GFP (Venus); Xenopus laevis calmodulin; synthetic myosin light chain kinase peptide (M13).
Depositor|Developer Nagai, Takeharu |
 
Sequence (full) RDB19877hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19877_B3Acp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Horikawa, K. et al., Nat. Methods 7 (9): 729-732, 2010) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Horikawa, K. et al., Nat. Methods 7 (9): 729-732, 2010)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19877 Yellow Cameleon-Nano65/pcDNA3 DNA solution


How to cite this biological resource

Materials & Methods section:

The Yellow Cameleon-Nano65/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19877).

Reference section:

Horikawa, K., Yamada, Y., Matsuda, T., Kobayashi, K., Hashimoto, M., Matsu-ura, T., Miyawaki, A., Michikawa, T., Mikoshiba, K., Nagai, T., Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano. Nat. Methods 7 (9): 729-732 (2010). PMID 20693999. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Horikawa, K., Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano. Nat. Methods 7 (9): 729-732 (2010). PMID 20693999. [link to RRC of NBRP]