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pGEX-4T-3-APPL1-N (#RDB19706)

Bacterial expression vector of mouse APPL1-N (amino acids 5-377) tagged with GST at N-terminus.

Clone info. Bacterial expression vector of mouse APPL1 (adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1)-N (amino acids 5-377) tagged with GST at N-terminus. See Fig.4C of Hiragi, S. et al. (2022) J. Cell Biol. 221, e202201114
Vector backbone pGEX-4T-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Appl1 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB19706zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ohbayashi, N. et al. Mol. Biol. Cell. 23, 669-678, 2012)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ohbayashi, N. et al. Mol. Biol. Cell. 23, 669-678, 2012)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19706 pGEX-4T-3-APPL1-N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-4T-3-APPL1-N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19706).

Reference section:

Hiragi, S., Matsui, T., Sakamaki, Y., Fukuda, M., TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1. J. Cell Biol. 21 (12): e202201114 (2022). PMID 36197338. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19706_B2Jjp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 (Pr0403)
Region: GST,thrombin site,insert 5'
Sequence file: RDB19706_B2Jja.seq check
>D09032A3_B2Jj_1_pGEX-F2_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNTATT GAAGCTATCC CNCAAATTGA TAAGTACTTG
   61 AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC
  121 GACCATCCTC CAAAATCGGA TCTGGTTCCG CGTGGATCCG ACAAGCTGCC CATTGAAGAG
  181 ACCCTGGAGG ACAGTCCGCA GACAAGGTCT TTACTTGGTG TATTTGAAGA AGATGCTACT
  241 GCTATTTCAA ATTATATGAA CCAATTGTAT CAAGCTATGC ATCGGATTTA TGATGCACAG
  301 AATGAATTGA GTGCAGCAAC ACACCTGACT TCGAAACTTT TGAAAGAATA TGAAAAACAG
  361 CGTTTTCCTT TGGGGGGTGA TGATGAAGTT ATGAGCTCTA CTTTGCAGCA GTTTTCCAAG
  421 GTCATAGATG AGCTTAGTTC TTGTCATGCA GTACTTTCAA CTCAACTTGC TGATGCCATG
  481 ATGTTCCCCA TTTCCCAGTT TAAAGAAAGA GATCTGAAAG AAATATTGAC ACTAAAGGAG
  541 GTATTTCAAA TTGCTAGTAA TGATCATGAT GCCGCAATTA ATAGGTACAG TCGGTTGTCA
  601 AAAAAGAGAG AAAATGACAA GGTAAAATAT GAAGTTACAG AGGATGTATA TACTTCCAGG
  661 AAAAAACAAC ACCAGACCAT GATGCATTAT TTTTGTGCAC TAAATACTCT TCAGTACAAG
  721 AAGAAAATAG CTTTGTTAGA ACCTCTACTG GGATACATGC AGGCTCAGAT AAGTTTCTTT
  781 AAGATGGGTT CTGANNATCT CAATGGACAA CTGGAAGANT TCTTAGCTAA CATTGGAACA
  841 AGTGTACAGA ATGTTCGAAG ANAAATGGAT GGTGATGTAG AGANCATGCA NCANANAATA
  901 GAAGANTTGG NNGTANCCNG TGANCCTTTA TATCTGCCTG ANCCGGNNCC CACNNNGTTT
  961 CCCATCNATC GAANNTTNNC CCNAAAGGNT GGANANNNNA ANGCTNNNAA NAAAANNNNN
1021 TNNGNNGNCN NNNNNNNNGN NNNNNNNNN
//
Primer: pGEX-RSEQ (Pr0077)
Region: insert 3'
Sequence file: RDB19706_B2Jjb.seq check
>D09032A3_B2Jj_1_pGEX-RSEQ_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNCNCGNN GCAGNNCGTC NGTCNGTCAC GATGCGGCCG CGAATTCACT
   61 AGTGATTTCA TGGCCGAGAT TGTCCTCCTG GACGCAGGCT CTCGTGTCTC TGCTGGAAAG
  121 ATGGGGAAGG AGTGACAGCT TCTAGAGCAG ACTGATTCAC TCGTGCAGCA GTTTCCTCTG
  181 GATTTTCACT CAAATATATT TGTTTAGATA TGTTGTTTAT TGTACAGATC CACTCTTCAT
  241 GGTCTTTTTT ACTCTCTGCT TGCAATATTG AAGATTTTTT TCCATCAAAA GAAGTGATCT
  301 GAAAACAGTA TCGTCTGTCT TCACAGTCCA CAGCCATCAC TGAACAGTTG TCTATGTCCA
  361 TGGCTAGGCC TCCTGCCACA TCTCCACGGG CTTGACTCAT TAAGTTTCCA CCCTGCGTGA
  421 AGTAAAACTG TCTATCCCAG GTAGATGACA CCAAGCCTGT TTTATTCCTA GCATTTAGGT
  481 ATCCAGCCTT TCGGGTTAAA TTTCGATTGA TGGGAAACTT GGTGGGATCC GGGTCAGGCA
  541 GATATAAAGG GTCACTGGCT ACCTCCAAGT CTTCTATTGT CTGCTGCATG GTCTCTACAT
  601 CACCATCCAT TTCTCTTCGA ACATTCTGTA CACTTGTTCC AATGTTAGCT AAGAATTCTT
  661 CCAGTTGTCC ATTGAGATTT TCAGAACCCA TCTTAAAGAA ACTTATCTGA NCCTGCATGT
  721 ATCCCAGTAG AGGTTCTAAC AAAGCTATTT TCTTCTTGTA CTGAAGAGTA TTTAGTGCAC
  781 AAAAATAATG CATCATGGTC TGGTGTTGTT TTTTCCTGGA AGTATATACA TCCTCTGTAA
  841 CTTCATATTT TACCTTGTCA TTTTCTCTCT TTTTTGACAA CCGACTGTAC CTATTAATTG
  901 CGGCATCNTG ATCATTACTA GCNATTTGAA ATANCNNCNT NANTGTCNAT ATTTCTTTCN
  961 NANNTCTTTN NTTNAACTGG GAAATGGGGN ANNNTCATGG NNNCANNNNN NTNNANTNNN
1021 ANGTANNGNN NNNNNNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: lacZ alpha,lacO,lac pro,lacI
Sequence file: RDB19706_B2Jjc.seq check
>D09032A3_B2Jj_1_pUC-M13_-100_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TNGNNANGCN NGGGTTTTCC CNGTCNCGAC GTTGTAAAAC
   61 GACGGCCAGT GAATCCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC
  121 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT
  181 GAGTGAGCTA ACTTACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
  241 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
  301 GGCGCCAGGG TGGTTTTTCT TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC
  361 GCCTGGCCCT GAGAGAGTTG CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA
  421 TCCTGTTTGA TGGTGGTTAA CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT
  481 CCCACTACCG AGATATCCGC ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG
  541 CCCAGCGCCA TCTGATCGTT GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC
  601 ATTTGCATGG TTTGTTGAAA ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC
  661 GGCTGAATTT GATTGCGAGT GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG
  721 ACAGAACTTA ATGGGCCCGC TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC
  781 TCCACGCCCA GTCGCGTANC GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG
  841 TCAGANACAT CNAGAAATAA CGCCNGANCA TTAGTGCANG CAGCTTCCAC AGCAATGGCA
  901 TCCTNNTCAT CCNGCGGANA GTTAATGATC AGCCNANNGA NGCGTTGCGC NNNAANANTG
  961 TGNACCGCCG CTTNNCNNNN TCNNNGNNNN TTCNNNCTAN NNTCNNCNNC ANNNNNCTGG
1021 NNCCNNNNNG ATNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hiragi, S., TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1. J. Cell Biol. 21 (12): e202201114 (2022). PMID 36197338. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]