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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-4T-3-APPL1-N

Bacterial expression vector of mouse APPL1-N (amino acids 5-377) tagged with GST at N-terminus.

Catalog number RDB19706
Resource name pGEX-4T-3-APPL1-N
Clone info. Bacterial expression vector of mouse APPL1 (adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1)-N (amino acids 5-377) tagged with GST at N-terminus. See Fig.4C of Hiragi, S. et al. (2022) J. Cell Biol. 221, e202201114
Vector backbone pGEX-4T-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Appl1 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Appl1

          Reference sequence
            
           
          Sequence (full) RDB19706zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ohbayashi, N. et al. Mol. Biol. Cell. 23, 669-678, 2012)
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ohbayashi, N. et al. Mol. Biol. Cell. 23, 669-678, 2012)。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19706 pGEX-4T-3-APPL1-N DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGEX-4T-3-APPL1-N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19706).

          Reference section:

          Hiragi, S., Matsui, T., Sakamaki, Y., Fukuda, M., TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1. J. Cell Biol. 21 (12): e202201114 (2022). PMID 36197338.

          Further references such as user reports and related articles (go to bottom)


          References and tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19706_B2Jjp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2 (Pr0403)
          Region: GST,thrombin site,insert 5'
          Sequence file: RDB19706_B2Jja.seq check
          >D09032A3_B2Jj_1_pGEX-F2_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNTATT GAAGCTATCC CNCAAATTGA TAAGTACTTG
             61 AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC
            121 GACCATCCTC CAAAATCGGA TCTGGTTCCG CGTGGATCCG ACAAGCTGCC CATTGAAGAG
            181 ACCCTGGAGG ACAGTCCGCA GACAAGGTCT TTACTTGGTG TATTTGAAGA AGATGCTACT
            241 GCTATTTCAA ATTATATGAA CCAATTGTAT CAAGCTATGC ATCGGATTTA TGATGCACAG
            301 AATGAATTGA GTGCAGCAAC ACACCTGACT TCGAAACTTT TGAAAGAATA TGAAAAACAG
            361 CGTTTTCCTT TGGGGGGTGA TGATGAAGTT ATGAGCTCTA CTTTGCAGCA GTTTTCCAAG
            421 GTCATAGATG AGCTTAGTTC TTGTCATGCA GTACTTTCAA CTCAACTTGC TGATGCCATG
            481 ATGTTCCCCA TTTCCCAGTT TAAAGAAAGA GATCTGAAAG AAATATTGAC ACTAAAGGAG
            541 GTATTTCAAA TTGCTAGTAA TGATCATGAT GCCGCAATTA ATAGGTACAG TCGGTTGTCA
            601 AAAAAGAGAG AAAATGACAA GGTAAAATAT GAAGTTACAG AGGATGTATA TACTTCCAGG
            661 AAAAAACAAC ACCAGACCAT GATGCATTAT TTTTGTGCAC TAAATACTCT TCAGTACAAG
            721 AAGAAAATAG CTTTGTTAGA ACCTCTACTG GGATACATGC AGGCTCAGAT AAGTTTCTTT
            781 AAGATGGGTT CTGANNATCT CAATGGACAA CTGGAAGANT TCTTAGCTAA CATTGGAACA
            841 AGTGTACAGA ATGTTCGAAG ANAAATGGAT GGTGATGTAG AGANCATGCA NCANANAATA
            901 GAAGANTTGG NNGTANCCNG TGANCCTTTA TATCTGCCTG ANCCGGNNCC CACNNNGTTT
            961 CCCATCNATC GAANNTTNNC CCNAAAGGNT GGANANNNNA ANGCTNNNAA NAAAANNNNN
          1021 TNNGNNGNCN NNNNNNNNGN NNNNNNNNN
          //
          Primer: pGEX-RSEQ (Pr0077)
          Region: insert 3'
          Sequence file: RDB19706_B2Jjb.seq check
          >D09032A3_B2Jj_1_pGEX-RSEQ_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNCNCGNN GCAGNNCGTC NGTCNGTCAC GATGCGGCCG CGAATTCACT
             61 AGTGATTTCA TGGCCGAGAT TGTCCTCCTG GACGCAGGCT CTCGTGTCTC TGCTGGAAAG
            121 ATGGGGAAGG AGTGACAGCT TCTAGAGCAG ACTGATTCAC TCGTGCAGCA GTTTCCTCTG
            181 GATTTTCACT CAAATATATT TGTTTAGATA TGTTGTTTAT TGTACAGATC CACTCTTCAT
            241 GGTCTTTTTT ACTCTCTGCT TGCAATATTG AAGATTTTTT TCCATCAAAA GAAGTGATCT
            301 GAAAACAGTA TCGTCTGTCT TCACAGTCCA CAGCCATCAC TGAACAGTTG TCTATGTCCA
            361 TGGCTAGGCC TCCTGCCACA TCTCCACGGG CTTGACTCAT TAAGTTTCCA CCCTGCGTGA
            421 AGTAAAACTG TCTATCCCAG GTAGATGACA CCAAGCCTGT TTTATTCCTA GCATTTAGGT
            481 ATCCAGCCTT TCGGGTTAAA TTTCGATTGA TGGGAAACTT GGTGGGATCC GGGTCAGGCA
            541 GATATAAAGG GTCACTGGCT ACCTCCAAGT CTTCTATTGT CTGCTGCATG GTCTCTACAT
            601 CACCATCCAT TTCTCTTCGA ACATTCTGTA CACTTGTTCC AATGTTAGCT AAGAATTCTT
            661 CCAGTTGTCC ATTGAGATTT TCAGAACCCA TCTTAAAGAA ACTTATCTGA NCCTGCATGT
            721 ATCCCAGTAG AGGTTCTAAC AAAGCTATTT TCTTCTTGTA CTGAAGAGTA TTTAGTGCAC
            781 AAAAATAATG CATCATGGTC TGGTGTTGTT TTTTCCTGGA AGTATATACA TCCTCTGTAA
            841 CTTCATATTT TACCTTGTCA TTTTCTCTCT TTTTTGACAA CCGACTGTAC CTATTAATTG
            901 CGGCATCNTG ATCATTACTA GCNATTTGAA ATANCNNCNT NANTGTCNAT ATTTCTTTCN
            961 NANNTCTTTN NTTNAACTGG GAAATGGGGN ANNNTCATGG NNNCANNNNN NTNNANTNNN
          1021 ANGTANNGNN NNNNNNNN
          //
          Primer: pUC-M13_-100 (Pr0310)
          Region: lacZ alpha,lacO,lac pro,lacI
          Sequence file: RDB19706_B2Jjc.seq check
          >D09032A3_B2Jj_1_pUC-M13_-100_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TNGNNANGCN NGGGTTTTCC CNGTCNCGAC GTTGTAAAAC
             61 GACGGCCAGT GAATCCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC
            121 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT
            181 GAGTGAGCTA ACTTACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
            241 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
            301 GGCGCCAGGG TGGTTTTTCT TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC
            361 GCCTGGCCCT GAGAGAGTTG CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA
            421 TCCTGTTTGA TGGTGGTTAA CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT
            481 CCCACTACCG AGATATCCGC ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG
            541 CCCAGCGCCA TCTGATCGTT GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC
            601 ATTTGCATGG TTTGTTGAAA ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC
            661 GGCTGAATTT GATTGCGAGT GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG
            721 ACAGAACTTA ATGGGCCCGC TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC
            781 TCCACGCCCA GTCGCGTANC GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG
            841 TCAGANACAT CNAGAAATAA CGCCNGANCA TTAGTGCANG CAGCTTCCAC AGCAATGGCA
            901 TCCTNNTCAT CCNGCGGANA GTTAATGATC AGCCNANNGA NGCGTTGCGC NNNAANANTG
            961 TGNACCGCCG CTTNNCNNNN TCNNNGNNNN TTCNNNCTAN NNTCNNCNNC ANNNNNCTGG
          1021 NNCCNNNNNG ATNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hiragi, S., TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1. J. Cell Biol. 21 (12): e202201114 (2022). PMID 36197338.
          reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

          2023.05.22

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