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pEGFP-C1-hTBC1D18-R584K  (#RDB19705)

Retroviral expression vector of human TBC1D18 GAP activity-deficient mutant tagged with EGFP at N-terminus.

Clone info. Retroviral expression vector of human TBC1D18 (TBC1 domain family, member 18) GAP activity-deficient R584K mutant tagged with EGFP at N-terminus. See Fig.3A of Hiragi, S. et al. (2022) J. Cell Biol. 221, e202201114
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human RABGAP1L cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB19705zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells. 11, 1023-1037, 2006)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19705 pEGFP-C1-hTBC1D18-R584K  DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-hTBC1D18-R584K  was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19705).

Reference section:

Hiragi, S., Matsui, T., Sakamaki, Y., Fukuda, M., TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1. J. Cell Biol. 21 (12): e202201114 (2022). PMID 36197338. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19705_B2Jjp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB19705_B2Jja.seq check
>D09032A2_B2Jj_1_EGFP-C2_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNGNN CNCNTGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGNTCA
   61 CTCTCGGCAT GGANGAGCTG TACAAGTCCG GACTCAGATC CATGGAGGTC AGAGCTTCAT
  121 TACAGAAGGT TAGTGGATCA TCTGATTCTG TGGCTACAAT GAACAGTGAA GAATTTGTTT
  181 TGGTTCCTCA GTATGCAGAT GATAATTCTA CAAAACATGA AGAAAAACCT CAACTGAAGA
  241 TAGTTTCTAA TGGTGATGAA CAATTGGAAA AAGCCATGGA AGAGATTTTG AGAGATTCCG
  301 AGAAAAGGCC AAGCAGTCTT CTTGTTGATT GTCAAAGTTC CAGTGAGATT TCAGACCATT
  361 CGTTTGGAGA TATTCCAGCC AGCCAAACAA ATAAGCCATC TCTTCAGTTA ATTTTGGATC
  421 CGTCTAACAC AGAAATTTCT ACACCCAGAC CATCTTCTCC AGGTGGACTA CCTGAAGAAG
  481 ATAGTGTTTT ATTTAATAAA CTGACCTACT TAGGATGTAT GAAGGTTTCT TCCCCACGTA
  541 ATGAAGTAGA GGCTTTACGG GCAATGGCAA CCATGAAATC TTCCAGTCAA TACCCCTTTC
  601 CTGTTACCCT GTATGTACCA AATGTTCCAG AAGGTTCTGT GAGAATTATA GACCAATCCA
  661 GCAATGTGGA GATAGCATCT TTTCCAATCT ATAAGGTGTT ATTCTGTGCA CGTGGACATG
  721 ACGGAACAAC AGAGAGCAAT TGCTTTGCAT TTACAGAGAG TTCCCATGGT TCGGAAGAAT
  781 TTCANATACA TGTTTTCTCC TGTGAAATTA AAGANGCAGT AAGCAGAATT TTGTACAGTT
  841 TCTGTACAGC ATTCAAACGT TCTTCCAGAC AANNGTCTGA NGTTAAAGAN TCAGTTATTC
  901 CTACCCCCGA CAGTGATGTG TTTACCTTCA GTGTCTCCTT GGANGTAAAA GAANANNANN
961 GNAAANGAAA CTTTANCCNT GNNNNNNNNN N
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB19705_B2Jjb.seq check
>D09032A2_B2Jj_1_SV40polyA_R2_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNCNGNANN ANNNNNNTAA CNACNACNAT TGCATTCATT TTATGTTTCN
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT
  181 GCAGGCGGCC GCGAATTCAC TAGTGATTAC AGCTGGAGTT ACCTACAAAT ATACAAACTT
  241 GTATCTATCC ATTGGGTCTT CCTGTTGCAG CTGACTCTCT CGCATTGTCT GATATTCTTT
  301 CTCATATTTC TTCAGCTTCT TGGTTGGTAC TTTAATATTG CAAGCCTGCT CCATCAGTCT
  361 TCTTGCATTT TCCTCTGCCC TGTATCTCTT TGGAAGCTGA ACTCTAAAGA ACTTTAAAGC
  421 ACCTTCAAAA TCAGCCTGCA GAAGGTCTTC CTTTGAGGTC TTTAGGAGAG CCAAAGCTAC
  481 ATGAAAGATT ATGTTCAAAC CCTCACAAAG CAGTAAGTCA ATGATGTGGA ACACCATGCA
  541 GAGTGGGAAC TTGGCAGTAA AAAGAGTGAG AAACCACTGG GATGCATACA TATGAGCTTC
  601 CAGGTTCAGA TCAGAAAAAT GGCTATGCAG GTCCGGTAGC TGTTCCTGCA TTAGTCTCTC
  661 CAACTGGTAG AATTTGCAAT GAAGATCTTC GAAGTTGTTT CTGTAGAGGT CTCTCAAACC
  721 ATAGTCGTAC ATGATTTTCA CCAAAACACA GAATGCTTGT TCCTCTGGCA TATGCAGCAG
  781 TAATACAGCA GCAAGAAAAG ANTGCCCTTG ACAGTACCCA ATGTCTTCAT CATACACAGA
  841 GTANGCCTTG CAGATCTTAT AGAGCGATTC TTGACCATCT CCTCCAGTAT CTTTAAAGTA
  901 ATCNTGTGCG GGAAATGTCT TATGAATATC TCGAGTAANN NCACTCTCCT NGGCTGANTC
  961 CTTTGTGANN AGAATTCNGN ATCTATCCNG CANTGCCNNN NNGNCATNGN CANNCNGNCN
1021 NNTAACTGNN NNANNNNNNN CCNNNNNNGN NTNNNNNGNN NNNNNNNN
//
Primer: hTBC1D18_mid_R (Pr1294)
Region: insert mid
Sequence file: RDB19705_B2Jjc.seq check
>D09032A2_B2Jj_1_hTBC1D18_mid_R_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG AANGCTTGTT CCTCTGGCAT ATGCAGCAGT
   61 AATACAGCAG CAAGAAAAGA CTGCCCTTGA CAGTACCCAA TGTCTTCATC ATACACAGAG
  121 TAGGCCTTGC AGATCTTATA GAGCGATTCT TGACCATCTC CTCCAGTATC TTTAAAGTAA
  181 TCATGTGCGG GAAATGTCTT ATGAATATCT CGAGTAATAA CACTCTCCTG GGCTGAGTCC
  241 TTTGTGATAA GAATTCGGTA TCTATCCAGC ATTGCCTGGT TGTCATGGCA GCCTGCCAAT
  301 AACTGCCATA CCTCTGCCCT CAATGCTTCA GGGACACCAC TCTTCACCAG AGTAGACAGC
  361 CCTTTCGGTC GTGCACCAAG GTTACTGTGC CATTTTCCTA GCAACTCTCC CCAAGAATAC
  421 AGGATCTTCT CAGGACAATC CTTAGACACA TCACCTGTTC CACTTGAGAG TTCATTATCA
  481 CTCTCCTCTT CTGCTTCATC ATCCTGGGGT GACATTGGAN CCCCTCCACT AGTANGAGTG
  541 ACTGGTTCCT CCTTGTCAGA NTCTCGCTGT AGACTCACCA CCTCATATAT TGCATCTCCA
  601 GCNNTGNTAT GGNCTTTTCC NTCANANNGN TTCNATCTCN TGAAGAAAGT CTCTGTGAAA
  661 NTCNTTCTGC TGAAATANCA AANTCGCTCA TTTGNNNGGN ANNNACNGNN TANTGNCTNC
721 NNNNNNAAGC NANNNGNNTN CNNNNNNTCN GNGANNNNNN TATCCNNNNN NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB19705_B2Jjd.seq check
>D09032A2_B2Jj_1CMV_Forward_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTTGNNNCNA ATGGGCGGNA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
  121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
  241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
  301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
  361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
  421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
  481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
  541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
  601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
  661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
  721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
  781 TGCTGGAGTT CGTGACCGCC NNNNNNATCA CTCTCGGCAT GGACGAGCTG TACNANGTCC
  841 GGACTCNGAT NCATGGAGGT CANANCTTCN TTACAGAAGG NTAGTGGATC ATCTGATTCT
  901 GTGGCTACNN NGAANAGNGA ANANNTTGNT TTGGNNCCTC AGTATGCAGA TGANAATTCT
  961 ACAAAANNTG ANNNAAANNN TCNNCTGANN NNANNTTCNA ATNGNGNNGN ACNNTTGGAA
1021 AAANNNANNG NNNNANNNTT TGANNNNNNN NNN
//
Primer: VN_F1 (Pr0992)
Region: EGFP 3',insert 5'
Sequence file: RDB19705_B2Jje.seq check
>D09032A2_B2Jj_1_VN_F1_F02_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCNNNNNNNT CTATATNNTG GCCGANAAGC AGAAGAACGG
   61 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  121 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  181 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
  241 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG
  301 ACTCAGATCC ATGGAGGTCA GAGCTTCATT ACAGAAGGTT AGTGGATCAT CTGATTCTGT
  361 GGCTACAATG AACAGTGAAG AATTTGTTTT GGTTCCTCAG TATGCAGATG ATAATTCTAC
  421 AAAACATGAA GAAAAACCTC AACTGAAGAT AGTTTCTAAT GGTGATGAAC AATTGGAAAA
  481 AGCCATGGAA GAGATTTTGA GAGATTCCGA GAAAAGGCCA AGCAGTCTTC TTGTTGATTG
  541 TCAAAGTTCC AGTGAGATTT CAGACCATTC GTTTGGAGAT ATTCCAGCCA GCCAAACAAA
  601 TAAGCCATCT CTTCAGTTAA TTTTGGATCC GTCTAACACA GAAATTTCTA CACCCAGACC
  661 ATCTTCTCCA GGTGGACTAC CTGAAGAAGA TAGTGTTTTA TTTAATAAAC TGACCTACTT
  721 AGGATGTATG AAGGTTTCTT CCCCACGTAA TGAAGTAGAG GCTTTACGGG CAATGGCAAC
  781 CATGAAATCT TCCAGTCNAT ACCCCTTTCC TGTTACCCTG TATGTACCAA ATGTTCCAGA
  841 ANGTTCTGTG AGANNTATAG ACNNNTCNAG CAATGTGGAN ATAGCATCTT TTCNNATCTA
  901 TAANGNGTTA TTCTGTGCAC GTGGNNNNTG NCGGAACNAC NNAGAGCNAT TGCTTTGCNT
  961 TTACAGANAG TTNCNANGGN TNCGNAANAA TTTNNNANAC NTGNTTTCNN NNNNGAAATT
1021 NAANNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hiragi, S., TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1. J. Cell Biol. 21 (12): e202201114 (2022). PMID 36197338. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]