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pcDNA/TO/myc-PRC1_Y464E (#RDB19643)

Expression vector of human PRC1 phosphomimetic mutant (Y464E), Tet inducible.

Clone info. Expression vector of human PRC1 phosphomimetic mutant with a glutamate substitution for Tyr 464 (Y464E), myc-tag at N-terminus, Tet operator. See Fig.4 of Morii, M. et al. Sci. Rep. 11 (1): 2616, 2021.
Comment Expression was confirmed by the depositor with western blotting, Immunostaining.
Vector backbone pcDNA4/TO (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human PRC1 cDNA.
Depositor|Developer Honda, Takuya |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Morii, M. et al. Sci. Rep. 11(1) :2616, 2021)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Morii, M. et al. Sci. Rep. 11(1) :2616, 2021)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19643 pcDNA/TO/myc-PRC1_Y464E Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pcDNA/TO/myc-PRC1_Y464E was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19643).

Reference section:

Morii, M., Kubota, S., Hasegawa, C., Takeda, Y., Kometani, S., Enomoto, K., Suzuki, T., Yanase, S., Sato, R., Akatsu, A., Hirata, K., Honda, T., Kuga, T., Tomonaga, T., Nakayama, Y., Yamaguchi, N., Yamaguchi, N., Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle. Sci. Rep. 11 (1): 2616 (2021). PMID 33510346. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

original Morii, M., Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle. Sci. Rep. 11 (1): 2616 (2021). PMID 33510346. [link to RRC of NBRP]