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pCAG-Flpo (#RDB19603)

Expression vector of S. cerevisiae Flippase.


Drawn by SnapGene® software
Sequence (full) RDB19603hts01.seq
Publication Sakamoto, M., STAR Protoc. 3 (2): 101421 (2022). [link to RRC of NBRP]
Test sheet RDB19603_B2Fmp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of S. cerevisiae Flippase, SV40-polyA signal, CAG promoter.
Vector backbone pCAGpro-C1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammaliancell)
Gene/insert name Saccharomyces cerevisiae Flippase
Depositor|Developer Bito, Haruhiko | Sakamoto, Masayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Sakamoto, M., STAR Protoc. 3 (2): 101421 (2022)) designated by the DEPOSITOR is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Sakamoto, M., STAR Protoc. 3 (2): 101421 (2022)) を引用する。

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RDB19603 pCAG-Flpo DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAG-Flpo was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19603).

Reference section:

Sakamoto, M., Ota, K., Kondo, Y., Okamura, M., Fujii, H., Bito, H., In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. STAR Protoc. 3 (2): 101421 (2022). PMID 35693213. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sakamoto, M., In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. STAR Protoc. 3 (2): 101421 (2022). PMID 35693213. [link to RRC of NBRP]