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pMRX-puro-3×FLAG-UACA-delta ANKR

Retroviral expression vector of mouse UACA ANKR domain-deletion mutant tagged with 3xFLAG at N-terminus.

Catalog number RDB19595
Resource name pMRX-puro-3×FLAG-UACA-delta ANKR
Clone info. Retroviral expression vector of mouse ANKR domain-deletion UACA mutant tagged with 3xFLAG at N-terminus. See Fig4A of Matsui, T. et al. Cell Rep. 39 (9): 110875 (2022).
Vector backbone pMRX-puro (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name mouse Uaca cDNA.
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Uaca

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Matsui, T. et al. Cell Rep. 39, 110875, 2022)
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Matsui, T. et al. Cell Rep. 39, 110875, 2022)。
          付加的提供条件:
          pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19595 pMRX-puro-3×FLAG-UACA-delta ANKR Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pMRX-puro-3×FLAG-UACA-delta ANKR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19595).

          Reference section:

          Matsui, T., Sakamaki, Y., Nakashima, S., Fukuda, M., Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells. Cell Rep. 2022 May 31;39(9):110875 (2022). PMID 35649370.

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Matsui, T., Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells. Cell Rep. 2022 May 31;39(9):110875 (2022). PMID 35649370.
          reference Mori, Y., Small GTPase Rab39A interacts with UACA and regulates the retinoic acid-induced neurite morphology of Neuro2A cells. Biochem. Biophys. Res. Commun. 435 (1): 113-119 (2013). PMID 23624502.

          2023.05.22

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