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pGEXM-hVAT1 (43-393) (#RDB19566)

Bacterial expression vector of human VAT1 mutant (a.a 43-393 residues) tagged with GST at N-terminus.

Clone info. Bacterial expression vector of human Vesicle amine transport-1 (VAT1) mutant (a.a 43-393 residues) tagged with GST at N-terminus, IPTG inducible, tag cut site by HRV 3C protease. See Fig.S9 of Kim, YK. et al. Sci. Rep. 11 (1): 2120, 2021.
Vector backbone pGEXM (plasmid)
Selectable markers Ampicillin
Gene/insert name human VAT1 cDNA
Depositor|Developer Hakoshima, Toshio | Kim, Sun-Yong |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Kim. SY. et. ai., Sci. Rep.11(1): 2120,, 2021) The availability of the BIOLOGICAL RESOURCE is restricted to a RECIPIENT of a not-for profit organization for a not-for-profit research.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kim. SY. et. ai., Sci. Rep.11(1): 2120,, 2021)。学術機関の非営利学術研究に限る。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19566 pGEXM-hVAT1 (43-393) Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pGEXM-hVAT1 (43-393) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19566).

Reference section:

Kim, S.Y., Mori, T., Chek, M.F., Furuya, S., Matsumoto, K., Yajima, T., Ogura, T., Hakoshima, T., journalStructural insights into vesicle amine transport-1 (VAT-1) as a member of the NADPH-dependent quinone oxidoreductase family. Sci. Rep. 11 (1): 2120 (2021). PMID 33483563. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kim, S.Y., journalStructural insights into vesicle amine transport-1 (VAT-1) as a member of the NADPH-dependent quinone oxidoreductase family. Sci. Rep. 11 (1): 2120 (2021). PMID 33483563. [link to RRC of NBRP]