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pBS-CaMKIIpro-R-CaMP2-WPRE (#RDB19475)

Expression vector of genetically encoded calcium indicator, R-CaMP2 (red).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Inoue, M., Nat Methods 12 (1): 64-70 (2015). PMID 25419959. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name R-CaMP2
Clone info. Expression vector of genetically encoded calcium indicator, R-CaMP2 (red).
Comment Expression was confirmed by the depositor by the transfection to cultured cells.
Vector backbone pBluescriptII (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Camk2a genomic DNA rat Calm2 cDNA; Discosoma sp. RFP (cpApple) cDNA
Reference of insert sequence KR149062 (DDBJ accession number)
Depositor|Developer Bito, Haruhiko | Inoue, Masatoshi |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Inoue, M. et al., Nat. Methods, 12 (1): 64-70, 2015)
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Catalog # Resource name Shipping form Fee
RDB19475 pBS-CaMKIIpro-R-CaMP2-WPRE DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The pBS-CaMKIIpro-R-CaMP2-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19475).

Reference section:

Inoue, M., Takeuchi, A., Horigane, S., Ohkura, M., Gengyo-Ando, K., Fujii, H., Kamijo, S., Takemoto-Kimura, S., Kano, M., Nakai, J., Kitamura, K., Bito, H., Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods 12 (1): 64-70 (2015). PMID 25419959. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Inoue, M., Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods 12 (1): 64-70 (2015). PMID 25419959. [PubMed] [Article] [RRC of NBRP]