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pBS-CaMKIIpro-R-CaMP2-WPRE

Expression vector of genetically encoded calcium indicator, R-CaMP2 (red).

Catalog number RDB19475
Resource name pBS-CaMKIIpro-R-CaMP2-WPRE
Alternative name R-CaMP2
Clone info. Expression vector of genetically encoded calcium indicator, R-CaMP2 (red).
Comment Expression was confirmed by the depositor by the transfection to cultured cells.
Vector backbone pBluescriptII (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Camk2a genomic DNA rat Calm2 cDNA; Discosoma sp. RFP (cpApple) cDNA
Reference of insert sequence KR149062 (DDBJ accession number)
Depositor|Developer Bito, Haruhiko | Inoue, Masatoshi |
 
Sequence (full) RDB19475hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19475_B2A4p1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Inoue, M. et al., Nat. Methods, 12 (1): 64-70, 2015)
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提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Inoue, M. et al., Nat. Methods, 12 (1): 64-70, 2015)。
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RDB19475 pBS-CaMKIIpro-R-CaMP2-WPRE DNA solution

How to cite this biological resource

Materials & Methods section:

The pBS-CaMKIIpro-R-CaMP2-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19475).

Reference section:

Inoue, M., Takeuchi, A., Horigane, S., Ohkura, M., Gengyo-Ando, K., Fujii, H., Kamijo, S., Takemoto-Kimura, S., Kano, M., Nakai, J., Kitamura, K., Bito, H., Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods 12 (1): 64-70 (2015). PMID 25419959. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Inoue, M., Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods 12 (1): 64-70 (2015). PMID 25419959. [link to RRC of NBRP]