Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pBS-CaMKIIpro-XCaMP-R-WPRE (#RDB19474)

Expression vector of genetically encoded calcium indicator, XCaMP-R (red).

Alternative name XCaMP-R
Clone info. Expression vector of genetically encoded calcium indicator, XCaMP-R (red).
Comment Expression was confirmed by the depositor by the transfection to cultured cells.
Vector backbone pBluescriptII (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Camk2a genomic DNA rat Calm2 cDNA Discosoma sp. RFP (mApple)
Reference of insert sequence MK770162 (DDBJ accession number)
Depositor|Developer Bito, Haruhiko | Inoue, Masatoshi |
 
Sequence (full) RDB19474hts01.seq checkAssembled from experimentally sequenced data.
Publication Inoue, M., Cell 177 (5): 1346-1360.e24 (2019). [link to RRC of NBRP]
Test sheet RDB19474_B2A4p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
In order to receive this biological resource, please request the Exclusive MTA to us.
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Inoue, M. et al., Cell, 177 (5): 1346-1360, e24, 2019)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Inoue, M. et al., Cell, 177 (5): 1346-1360, e24, 2019)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19474 pBS-CaMKIIpro-XCaMP-R-WPRE DNA solution

How to cite this biological resource

Materials & Methods section:

The pBS-CaMKIIpro-XCaMP-R-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19474).

Reference section:

Inoue, M., Takeuchi, A., Manita, S., Horigane, S.I., Sakamoto, M., Kawakami, R., Yamaguchi, K., Otomo, K., Yokoyama, H., Kim, R., Yokoyama, T., Takemoto-Kimura, S., Abe, M., Okamura, M., Kondo, Y., Quirin, S., Ramakrishnan, C., Imamura, T., Sakimura, K., Nemoto, T., Kano, M., Fujii, H., Deisseroth, K., Kitamura, K., Bito, H., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Inoue, M., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068. [link to RRC of NBRP]