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pBS-CaMKIIpro-XCaMP-R-WPRE (#RDB19474)

Expression vector of genetically encoded calcium indicator, XCaMP-R (red).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Inoue, M., Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name XCaMP-R
Clone info. Expression vector of genetically encoded calcium indicator, XCaMP-R (red).
Comment Expression was confirmed by the depositor by the transfection to cultured cells.
Vector backbone pBluescriptII (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Camk2a genomic DNA rat Calm2 cDNA Discosoma sp. RFP (mApple)
Reference of insert sequence MK770162 (DDBJ accession number)
Depositor|Developer Bito, Haruhiko | Inoue, Masatoshi |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Inoue, M. et al., Cell, 177 (5): 1346-1360, e24, 2019)
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Catalog # Resource name Shipping form Fee
RDB19474 pBS-CaMKIIpro-XCaMP-R-WPRE DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The pBS-CaMKIIpro-XCaMP-R-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19474).

Reference section:

Inoue, M., Takeuchi, A., Manita, S., Horigane, S.I., Sakamoto, M., Kawakami, R., Yamaguchi, K., Otomo, K., Yokoyama, H., Kim, R., Yokoyama, T., Takemoto-Kimura, S., Abe, M., Okamura, M., Kondo, Y., Quirin, S., Ramakrishnan, C., Imamura, T., Sakimura, K., Nemoto, T., Kano, M., Fujii, H., Deisseroth, K., Kitamura, K., Bito, H., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Inoue, M., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068. [PubMed] [Article] [RRC of NBRP]