Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBS-CaMKIIpro-XCaMP-B-WPRE

Expression vector of genetically encoded calcium indicator, XCaMP-B (blue).

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Inoue, M. et al., Cell, 177 (5): 1346-1360, e24, 2019)
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提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Inoue, M. et al., Cell, 177 (5): 1346-1360, e24, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19473_B2A4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: insert 5'(CaMKII pro 5') Sequence file: RDB19473_B2A4a.seq
>19473_19473_B2A4_3_pUC-M13_-100_E03.ab1     1 NNNNNNNNNN NCCNGGGTTT TCCCNGTCNC GACGTTGTAA AACGACGGCC AGTGAGCGCG    61 CGTAATACGA CTCACTATAG GGCGAATTGG AGCTCCACCG CGGTGGCGGC CGCATGCATC   121 ATTATGGCCT TAGGTCACTT CATCTCCATG GGGTTCTTCT TCTGATTTTC TAGAAAATGA   181 GATGGGGGTG CAGAGAGCTT CCTCAGTGAC CTGCCCAGGG TCACATCAGA AATGTCAGAG   241 CTAGAACTTG AACTCAGATT ACTAATCTTA AATTCCATGC CTTGGGGGCA TGCAAGTACG   301 ATATACAGAA GGAGTGAACT CATTAGGGCA GATGACCAAT GAGTTTAGGA AAGAAGAGTC   361 CAGGGCAGGG TACATCTACA CCACCCGCCC AGCCCTGGGT GAGTCCAGCC ACGTTCACCT   421 CATTATAGTT GCCTCTCTCC AGTCCTACCT TGACGGGAAG CACAAGCAGA AACTGGGACA   481 GGAGCCCCAG GAGACCAAAT CTTCATGGTC CCTCTGGGAG GATGGGTGGG GAGAGCTGTG   541 GCAGAGGCCT CAGGAGGGGC CCTGCTGCTC AGTGGTGACA GATAGGGGTG AGAAAGCAGA   601 CAGAGTCATT CCGTCAGCAT TCTGGGTCTG TTTGGTACTT CTTCTCACGC TAAGGTGGCG   661 GTGTGATATG CACAATGGCT AAAAAGCAGG GAGAGCTGGA AAGAAACAAG GACAGAGACA   721 GANGCCAAGT CAACCAGACC AATTCCCAGA NGAAGCAAAG AAACCATTAC AGAGACTACN   781 AGGGGGAANG GAANGAGAGA TGANTTAGCT NNNNNTGTAA ACCTTANAAC CCAGCTGTTG   841 NCAGGGCAAC GGGGCAATAC CTGTCTCTTC NNANGANNNG AANTTGCCNG GGNNACTACN   901 TCCTGTCTTT CTCNAGGNNC NTCCCAGANN GTGNCACCCA CTANCCGTTA NNNTANCANC   961 TNNNTCTTTG CNNNNCTTNA TCCNNTCNNN NCNGNNNAAN NGNNNNTATA NNNNNANGNN 1021 NGGGNNNNNN NNNN //
Primer: reverse-2 (Pr0100) Region: insert 3'(SV40 pA,WPRE) Sequence file: RDB19473_B2A4b.seq
>19473_19473_B2A4_3_reverse2_F03.ab1     1 NNNNNNNNNN CNCGNNNTTA ACCCTNNCTA AAGGGAACAA AAGCTGGGTA CTTAATTAAT    61 CCGGAGAGTC GACGCTTCGA AACGAATTCC TGGTACCCTA CTAGTAGCTC GAGTTGGATC   121 CTTATCGATT TTACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC   181 CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT   241 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA   301 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGCTCGAAG   361 CGGCCGGCCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC   421 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGAT   481 TGAGGGCCGA AGGGACGTAG CAGAAGGACG TCCCGCGCAG AATCCAGGTG GCAACACAGG   541 CGAGCAGCCA TGGAAAGGAC GTCAGCTTCC CCGACAACAC CACGGAATTG TCAGTGCCCA   601 ACAGCCGAGC CCCTGTCCAG CAGCGGGCAA GGCAGGCGGC GATGAGTTCC GCCGTGGCAA   661 TAGGGAGGGG GAAAGCGAAA GTCCCGGAAA GGAGCTGACA GGTGGTGGCA ATGCCCCAAC   721 CAGTGGGGGT TGCGTCAGCA AACACAGTGC ACACCACGCC ACGTTGCCTG ACAACGGGCC   781 ACAACTCCTC ATAAAGAGAC AGCAACCAGG ANTTATACNA GGANGANAAA ATGAAAGCCA   841 TACGGGAAGC AATAGCATGA TACNAAGGCA TTNAAGCAGC GNATCNNCAT ANCGNAAAAN   901 GANCAACATA GTTNANAATA NCAGTCNATC TTTCNCAAAT TTTGNAATCC AGANGTTGAN   961 TNNCNNNNAC GCGTTCNCTT CNNNNNCNTC NTTTGNANNA ACNNNNCNNN NTTNANNNNN 1021 NNNTCCNNNN NNNNNN //
Primer: EGFP-C-rev (Pr0026) Region: insert mid(XCaMP-B 5',CaMKII pro 3') Sequence file: RDB19473_B2A4c.seq
>19473_19473_B2A4_3_EGFP-C_rev_H02_23_ABI24.ab1     1 NNNNNNNNNN NNTGGNTGCT CNGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG    61 ATGGGGGTGT TCTGCTGGTA GTGGTAGGCG AGCTGCACGC CGCCGTCCTC GATGTTGTGG   121 CGGATCTTGA AGTTCGCCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG   181 TCCATAAATG GGTTTCCAAA GGAACGCTTT CTCAGCATAG ACTTGACCAG GATCACAGTG   241 GTCCAGCTGG GGATGTGCTT GACGTCGACC ATGGTGGCGA GATCCTTATC GTCATCGTCG   301 TACAGATCCC GACCCATTTG CTGTCCACCA GTCATGCTAG CCATACCATG ATGATGATGA   361 TGATGAGAAC CCATGGTGGC GAGATCTGAG TCCGGACCGG TAGCGCTGCC CCCAGAACTA   421 GGGGCCACTC GCCTGCCCGT GCTCCTGAGT GCAAACGGAG AACCGGCTTG ACTGACGAGC   481 TTGGGGCTTC TGAGCAGGGC ACTGTGGCTG CTCACAGCCT CGCGAGCCTT CGTCAGCATC   541 CAGGTCCCCA TGGCAACCAC CTCCGAAACG CCCTGTTCCC GTTGCCCCGG TAACTGCCTC   601 CCCAACACCT GCCTGCCTTT CACTTTAAAA CCTGCTCCTC TTTGCCCTGG CCTTATATAC   661 ACAATACTGC AGGACTGTGT GGGCCCAGGA GCAAGTGGAN CCTGTTCCCC CAGGGTGANG   721 TACTGAGGGG GTTGGANAAG GGGANGCGAA CAAACTTAGT CCACAAGTGA TCATCGCTCT   781 TCCTACCTCC CCCACCTCCN ACTATAGGGN CCTTTTAACA GACGGGANGG GANTAAGTGG   841 GGCAAAGANG CAGTTGCTAT GGTAACGGCT AGTGGGTGNC ACATTCTGGG ANGGTCCTTN   901 AGAAAGANAG GANGTANTTA NCCNNNNNAC TTCATCTCCT CNGNANANAN AGGTATTGNC   961 CNNNNNNCCN NNNNACAGCN NGGNTNNNNA NNNTNNNNGG GGNNNNTNNN NCNTCNNNNN 1021 NNNNN //
Primer: PRE_rev (Pr0250) Region: insert mid(WPRE,XCaMP-B 3') Sequence file: RDB19473_B2A4d.seq
>19473_19473_B2A4_3_PRE_rev_G03.ab1     1 NNNNNNNNNN NNNNNGCNNC ATAGTTAAGA ATACCNGTCA ATCTTTCNCA AATTTTGTAA    61 TCCAGAGGTT GATTATCTAG TACGCGTTCA CTTCGCTGTC ATCATTTGTA CAAACTCTTC   121 GTAGTTTACC TGACCATCCC CATCGATGTC TGCTTCCCTG ATCATTTCAT CAACCTCTTC   181 ATCTGTTAAC TTCTCTCCAA GGTTTGTCAT CACGTGGCGA AGCTCTGCTG CACTGATGTA   241 GCCATTGCCA TCCTTATCAA ACACACGGAA CGCTTCTCTA ATTTCTTCTT CACTGTCTGT   301 GTCTTTCATT TTTCTTGCCA TCATTGTCAG GAACTCAGGG AAGTCGATTG TGCCATTACC   361 GTCGGCATCT ACTTCATTGA TCATGTCCTG CAGCTCTGCT TCTGTGGGGT TCTGCCCCAG   421 AGACCGCAGC ACCGTCCCCA GCTCCTTGGT TGTTATTGTC CCATCCCCGT CCTTGTCAAA   481 TAGGGAGAAA GCCTCTTTAA ATTCTGCGAT CTGCTCTTCA GTCAGTTGGT CCGTGTTGTA   541 CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT   601 GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGGTGCCGTC   661 GTCCTTGAAG AAGATGGTGC GCTCCTGGAT GTAGCCTTCG GGCATGGCGG ACTTGAAGAA   721 GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT GGCTCANGGT   781 GGTCACGANG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCANGGT   841 CAGCTTGCCG TTGGTGGCAT CGCCCTCACC CTCGCCGCGC ACGCTGAACT TGTGGCCGTT   901 TANGTCGCCG TCCAGCTCGA NCANGANGGG CACCACCCNN GNNAACAGCN CCNCGCCNTT   961 GCTCANNNNG CNNCNNCCGG NANCGCCCTN GNANAGCNNG TCCATGNNNA NNNTGNNNCN 1021 NNNNNCNGNN CACNNNNNNN NNN //
Primer: FP-C_F1 (Pr0772) Region: insert mid(XCaMP-B mid) Sequence file: RDB19473_B2A4e.seq
>19473_19473_B2A4_3_FP-C_F1_H03.ab1     1 NNNNNNNNNN NNTCCNNCTG GAGTTCGNGA CCGCCGCCGG GNTCACTCTC GGCATGGACG    61 AGCTGTACAA GGGCGGTACC GGAGGGAGCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG   121 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGC   181 GCGGCGAGGG TGAGGGCGAT GCCACCAACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA   241 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGAGCCAC GGCGTGCAGT   301 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG   361 AAGGCTACAT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCACCTAC AAGACCCGCG   421 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT   481 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACACGGAC CAACTGACTG   541 AAGAGCAGAT CGCAGAATTT AAAGAGGCTT TCTCCCTATT TGACAAGGAC GGGGNTGGGA   601 CAATAACAAC CAAGGAGCTG GGGACGGTGC TGCGGTCTCT GGGGCAGAAC CCCACAGAAG   661 CAGAGCTGCA GGACATGATC AATGAAGTAG ATGCCGACGG TAATGGCACA ATCGACTTCC   721 CTGAGTTCCT GACAATGATG GCAAGAAAAA TGAAAGACAC AGACAGTGAA GAANNNNNAG   781 AGAAGCGTTC CGTGTGTTTG ATAAGGNNGG CAATGGCTAC NTCAGTGCAG CAGANCTTCG   841 CCNCGTGATG ACNAACCTTG GANAGAAGTT NACAGATGAA GANGNTGATG AAATGATCAG   901 GGAANCANNN NTCNNNGGGG GNNGGTCNNN TAAACNNCNN ANANTTNGNA NNANTGNNGN   961 NNGCNNANNN NNCGNNNANN ANNNNNTCNN NNNCNNNNNN NNNAANNTNN NNNANNNNNN 1021 NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl