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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS-CaMKIIpro-XCaMP-B-WPRE (#RDB19473)

Expression vector of genetically encoded calcium indicator, XCaMP-B (blue).

Alternative name XCaMP-B
Clone info. Expression vector of genetically encoded calcium indicator, XCaMP-B (blue).
Comment Expression was confirmed by the depositor by the transfection to cultured cells.
Vector backbone pBluescriptII (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Camk2a genomic DNA rat Calm2 cDNA
Reference of insert sequence MK770164 (DDBJ accession number)
Depositor|Developer Bito, Haruhiko | Inoue, Masatoshi |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Inoue, M. et al., Cell, 177 (5): 1346-1360, e24, 2019)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Inoue, M. et al., Cell, 177 (5): 1346-1360, e24, 2019)。

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RDB19473 pBS-CaMKIIpro-XCaMP-B-WPRE DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBS-CaMKIIpro-XCaMP-B-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19473).

Reference section:

Inoue, M., Takeuchi, A., Manita, S., Horigane, S.I., Sakamoto, M., Kawakami, R., Yamaguchi, K., Otomo, K., Yokoyama, H., Kim, R., Yokoyama, T., Takemoto-Kimura, S., Abe, M., Okamura, M., Kondo, Y., Quirin, S., Ramakrishnan, C., Imamura, T., Sakimura, K., Nemoto, T., Kano, M., Fujii, H., Deisseroth, K., Kitamura, K., Bito, H., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19473_B2A4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: insert 5'(CaMKII pro 5')
Sequence file: RDB19473_B2A4a.seq check
>19473_19473_B2A4_3_pUC-M13_-100_E03.ab1
    1 NNNNNNNNNN NCCNGGGTTT TCCCNGTCNC GACGTTGTAA AACGACGGCC AGTGAGCGCG
   61 CGTAATACGA CTCACTATAG GGCGAATTGG AGCTCCACCG CGGTGGCGGC CGCATGCATC
  121 ATTATGGCCT TAGGTCACTT CATCTCCATG GGGTTCTTCT TCTGATTTTC TAGAAAATGA
  181 GATGGGGGTG CAGAGAGCTT CCTCAGTGAC CTGCCCAGGG TCACATCAGA AATGTCAGAG
  241 CTAGAACTTG AACTCAGATT ACTAATCTTA AATTCCATGC CTTGGGGGCA TGCAAGTACG
  301 ATATACAGAA GGAGTGAACT CATTAGGGCA GATGACCAAT GAGTTTAGGA AAGAAGAGTC
  361 CAGGGCAGGG TACATCTACA CCACCCGCCC AGCCCTGGGT GAGTCCAGCC ACGTTCACCT
  421 CATTATAGTT GCCTCTCTCC AGTCCTACCT TGACGGGAAG CACAAGCAGA AACTGGGACA
  481 GGAGCCCCAG GAGACCAAAT CTTCATGGTC CCTCTGGGAG GATGGGTGGG GAGAGCTGTG
  541 GCAGAGGCCT CAGGAGGGGC CCTGCTGCTC AGTGGTGACA GATAGGGGTG AGAAAGCAGA
  601 CAGAGTCATT CCGTCAGCAT TCTGGGTCTG TTTGGTACTT CTTCTCACGC TAAGGTGGCG
  661 GTGTGATATG CACAATGGCT AAAAAGCAGG GAGAGCTGGA AAGAAACAAG GACAGAGACA
  721 GANGCCAAGT CAACCAGACC AATTCCCAGA NGAAGCAAAG AAACCATTAC AGAGACTACN
  781 AGGGGGAANG GAANGAGAGA TGANTTAGCT NNNNNTGTAA ACCTTANAAC CCAGCTGTTG
  841 NCAGGGCAAC GGGGCAATAC CTGTCTCTTC NNANGANNNG AANTTGCCNG GGNNACTACN
  901 TCCTGTCTTT CTCNAGGNNC NTCCCAGANN GTGNCACCCA CTANCCGTTA NNNTANCANC
  961 TNNNTCTTTG CNNNNCTTNA TCCNNTCNNN NCNGNNNAAN NGNNNNTATA NNNNNANGNN
1021 NGGGNNNNNN NNNN
//
Primer: reverse-2 (Pr0100)
Region: insert 3'(SV40 pA,WPRE)
Sequence file: RDB19473_B2A4b.seq check
>19473_19473_B2A4_3_reverse2_F03.ab1
    1 NNNNNNNNNN CNCGNNNTTA ACCCTNNCTA AAGGGAACAA AAGCTGGGTA CTTAATTAAT
   61 CCGGAGAGTC GACGCTTCGA AACGAATTCC TGGTACCCTA CTAGTAGCTC GAGTTGGATC
  121 CTTATCGATT TTACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC
  181 CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT
  241 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
  301 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGCTCGAAG
  361 CGGCCGGCCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC
  421 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGAT
  481 TGAGGGCCGA AGGGACGTAG CAGAAGGACG TCCCGCGCAG AATCCAGGTG GCAACACAGG
  541 CGAGCAGCCA TGGAAAGGAC GTCAGCTTCC CCGACAACAC CACGGAATTG TCAGTGCCCA
  601 ACAGCCGAGC CCCTGTCCAG CAGCGGGCAA GGCAGGCGGC GATGAGTTCC GCCGTGGCAA
  661 TAGGGAGGGG GAAAGCGAAA GTCCCGGAAA GGAGCTGACA GGTGGTGGCA ATGCCCCAAC
  721 CAGTGGGGGT TGCGTCAGCA AACACAGTGC ACACCACGCC ACGTTGCCTG ACAACGGGCC
  781 ACAACTCCTC ATAAAGAGAC AGCAACCAGG ANTTATACNA GGANGANAAA ATGAAAGCCA
  841 TACGGGAAGC AATAGCATGA TACNAAGGCA TTNAAGCAGC GNATCNNCAT ANCGNAAAAN
  901 GANCAACATA GTTNANAATA NCAGTCNATC TTTCNCAAAT TTTGNAATCC AGANGTTGAN
  961 TNNCNNNNAC GCGTTCNCTT CNNNNNCNTC NTTTGNANNA ACNNNNCNNN NTTNANNNNN
1021 NNNTCCNNNN NNNNNN
//
Primer: EGFP-C-rev (Pr0026)
Region: insert mid(XCaMP-B 5',CaMKII pro 3')
Sequence file: RDB19473_B2A4c.seq check
>19473_19473_B2A4_3_EGFP-C_rev_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNTGGNTGCT CNGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG
   61 ATGGGGGTGT TCTGCTGGTA GTGGTAGGCG AGCTGCACGC CGCCGTCCTC GATGTTGTGG
  121 CGGATCTTGA AGTTCGCCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG
  181 TCCATAAATG GGTTTCCAAA GGAACGCTTT CTCAGCATAG ACTTGACCAG GATCACAGTG
  241 GTCCAGCTGG GGATGTGCTT GACGTCGACC ATGGTGGCGA GATCCTTATC GTCATCGTCG
  301 TACAGATCCC GACCCATTTG CTGTCCACCA GTCATGCTAG CCATACCATG ATGATGATGA
  361 TGATGAGAAC CCATGGTGGC GAGATCTGAG TCCGGACCGG TAGCGCTGCC CCCAGAACTA
  421 GGGGCCACTC GCCTGCCCGT GCTCCTGAGT GCAAACGGAG AACCGGCTTG ACTGACGAGC
  481 TTGGGGCTTC TGAGCAGGGC ACTGTGGCTG CTCACAGCCT CGCGAGCCTT CGTCAGCATC
  541 CAGGTCCCCA TGGCAACCAC CTCCGAAACG CCCTGTTCCC GTTGCCCCGG TAACTGCCTC
  601 CCCAACACCT GCCTGCCTTT CACTTTAAAA CCTGCTCCTC TTTGCCCTGG CCTTATATAC
  661 ACAATACTGC AGGACTGTGT GGGCCCAGGA GCAAGTGGAN CCTGTTCCCC CAGGGTGANG
  721 TACTGAGGGG GTTGGANAAG GGGANGCGAA CAAACTTAGT CCACAAGTGA TCATCGCTCT
  781 TCCTACCTCC CCCACCTCCN ACTATAGGGN CCTTTTAACA GACGGGANGG GANTAAGTGG
  841 GGCAAAGANG CAGTTGCTAT GGTAACGGCT AGTGGGTGNC ACATTCTGGG ANGGTCCTTN
  901 AGAAAGANAG GANGTANTTA NCCNNNNNAC TTCATCTCCT CNGNANANAN AGGTATTGNC
  961 CNNNNNNCCN NNNNACAGCN NGGNTNNNNA NNNTNNNNGG GGNNNNTNNN NCNTCNNNNN
1021 NNNNN
//
Primer: PRE_rev (Pr0250)
Region: insert mid(WPRE,XCaMP-B 3')
Sequence file: RDB19473_B2A4d.seq check
>19473_19473_B2A4_3_PRE_rev_G03.ab1
    1 NNNNNNNNNN NNNNNGCNNC ATAGTTAAGA ATACCNGTCA ATCTTTCNCA AATTTTGTAA
   61 TCCAGAGGTT GATTATCTAG TACGCGTTCA CTTCGCTGTC ATCATTTGTA CAAACTCTTC
  121 GTAGTTTACC TGACCATCCC CATCGATGTC TGCTTCCCTG ATCATTTCAT CAACCTCTTC
  181 ATCTGTTAAC TTCTCTCCAA GGTTTGTCAT CACGTGGCGA AGCTCTGCTG CACTGATGTA
  241 GCCATTGCCA TCCTTATCAA ACACACGGAA CGCTTCTCTA ATTTCTTCTT CACTGTCTGT
  301 GTCTTTCATT TTTCTTGCCA TCATTGTCAG GAACTCAGGG AAGTCGATTG TGCCATTACC
  361 GTCGGCATCT ACTTCATTGA TCATGTCCTG CAGCTCTGCT TCTGTGGGGT TCTGCCCCAG
  421 AGACCGCAGC ACCGTCCCCA GCTCCTTGGT TGTTATTGTC CCATCCCCGT CCTTGTCAAA
  481 TAGGGAGAAA GCCTCTTTAA ATTCTGCGAT CTGCTCTTCA GTCAGTTGGT CCGTGTTGTA
  541 CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT
  601 GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGGTGCCGTC
  661 GTCCTTGAAG AAGATGGTGC GCTCCTGGAT GTAGCCTTCG GGCATGGCGG ACTTGAAGAA
  721 GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT GGCTCANGGT
  781 GGTCACGANG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCANGGT
  841 CAGCTTGCCG TTGGTGGCAT CGCCCTCACC CTCGCCGCGC ACGCTGAACT TGTGGCCGTT
  901 TANGTCGCCG TCCAGCTCGA NCANGANGGG CACCACCCNN GNNAACAGCN CCNCGCCNTT
  961 GCTCANNNNG CNNCNNCCGG NANCGCCCTN GNANAGCNNG TCCATGNNNA NNNTGNNNCN
1021 NNNNNCNGNN CACNNNNNNN NNN
//
Primer: FP-C_F1 (Pr0772)
Region: insert mid(XCaMP-B mid)
Sequence file: RDB19473_B2A4e.seq check
>19473_19473_B2A4_3_FP-C_F1_H03.ab1
    1 NNNNNNNNNN NNTCCNNCTG GAGTTCGNGA CCGCCGCCGG GNTCACTCTC GGCATGGACG
   61 AGCTGTACAA GGGCGGTACC GGAGGGAGCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
  121 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGC
  181 GCGGCGAGGG TGAGGGCGAT GCCACCAACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA
  241 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGAGCCAC GGCGTGCAGT
  301 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
  361 AAGGCTACAT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCACCTAC AAGACCCGCG
  421 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
  481 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACACGGAC CAACTGACTG
  541 AAGAGCAGAT CGCAGAATTT AAAGAGGCTT TCTCCCTATT TGACAAGGAC GGGGNTGGGA
  601 CAATAACAAC CAAGGAGCTG GGGACGGTGC TGCGGTCTCT GGGGCAGAAC CCCACAGAAG
  661 CAGAGCTGCA GGACATGATC AATGAAGTAG ATGCCGACGG TAATGGCACA ATCGACTTCC
  721 CTGAGTTCCT GACAATGATG GCAAGAAAAA TGAAAGACAC AGACAGTGAA GAANNNNNAG
  781 AGAAGCGTTC CGTGTGTTTG ATAAGGNNGG CAATGGCTAC NTCAGTGCAG CAGANCTTCG
  841 CCNCGTGATG ACNAACCTTG GANAGAAGTT NACAGATGAA GANGNTGATG AAATGATCAG
  901 GGAANCANNN NTCNNNGGGG GNNGGTCNNN TAAACNNCNN ANANTTNGNA NNANTGNNGN
  961 NNGCNNANNN NNCGNNNANN ANNNNNTCNN NNNCNNNNNN NNNAANNTNN NNNANNNNNN
1021 NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Inoue, M., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068. [link to RRC of NBRP]