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pBS-CaMKIIpro-XCaMP-Y-WPRE (#RDB19472)

Expression vector of genetically encoded calcium indicator, XCaMP-Y (yellow).

Alternative name XCaMP-Y
Clone info. Expression vector of genetically encoded calcium indicator, XCaMP-Y (yellow).
Comment Expression was confirmed by the depositor by the transfection to cultured cells.
Vector backbone pBluescriptII (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Camk2a genomic DNA rat Calm2 cDNA
Reference of insert sequence MK770163 (DDBJ accession number)
Depositor|Developer Bito, Haruhiko | Inoue, Masatoshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Inoue, M. et al., Cell, 177 (5): 1346-1360, e24, 2019)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Inoue, M. et al., Cell, 177 (5): 1346-1360, e24, 2019)。

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RDB19472 pBS-CaMKIIpro-XCaMP-Y-WPRE Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pBS-CaMKIIpro-XCaMP-Y-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19472).

Reference section:

Inoue, M., Takeuchi, A., Manita, S., Horigane, S.I., Sakamoto, M., Kawakami, R., Yamaguchi, K., Otomo, K., Yokoyama, H., Kim, R., Yokoyama, T., Takemoto-Kimura, S., Abe, M., Okamura, M., Kondo, Y., Quirin, S., Ramakrishnan, C., Imamura, T., Sakimura, K., Nemoto, T., Kano, M., Fujii, H., Deisseroth, K., Kitamura, K., Bito, H., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Inoue, M., Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. Cell 177 (5): 1346-1360.e24 (2019). PMID 31080068. [link to RRC of NBRP]