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pFX-mito-EGFP

Expression vector of EGFP with mitochondria-targeting sequence (human COX8A) in mammalian cells.

Catalog number RDB19415
Resource name pFX-mito-EGFP
Clone info. Expression vector of EGFP with mitochondria-targeting sequence (human cytochrome c oxidase subunit VIII; COX8A, simply called mito hereafter) in mammalian cells.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pFX (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human COX8A cDNA
Depositor|Developer Ohba, Yusuke |
 
Sequence (full) RDB19415hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19415_B1Kfp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)。
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RDB19415 pFX-mito-EGFP DNA solution


How to cite this biological resource

Materials & Methods section:

The pFX-mito-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19415).

Reference section:

Kashiwagi, S., Fujioka, Y., Satoh, A.O., Yoshida, A., Fujioka, M., Nepal, P., Tsuzuki, A., Aoki, O., Paudel, S., Sasajima, H., Ohba, Y., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Kashiwagi, S., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740. [link to RRC of NBRP]