Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFX-beta-tubulin-SECFP

Expression vector of SECFP with microtuble-targeting sequence (human TUBB4B) in mammalian cells.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19413_B1Kfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro, insert 5'(beta-tubulin 5') Sequence file: RDB19413_B1Kfa.seq
>D08242A4_B1Kf_3_CMV_Forward_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNC NAATGGGCGG TAGGCNNTGT ACGGTGGGAG    61 GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTTCTAG   121 AGCCATGCTC GAGACCACCA TGAGGGAAAT CGTGCACTTG CAGGCCGGGC AGTGCGGCAA   181 CCAAATCGGC GCCAAGTTTT GGGAGGTGAT CAGCGATGAG CACGGCATCG ACCCCACGGG   241 CACCTACCAC GGGGACAGCG ACCTGCAGCT GGAACGCATC AACGTGTACT ACAATGAGGC   301 CACCGGCGGC AAGTACGTGC CCCGCGCCGT GCTCGTGGAT CTGGAGCCCG GCACCATGGA   361 CTCCGTGCGC TCGGGGCCCT TCGGGCAGAT CTTCCGGCCG GACAACTTCG TTTTCGGTCA   421 GAGTGGTGCT GGGAACAACT GGGCCAAGGG GCACTACACA GAAGGCGCGG AGCTGGTGGA   481 CTCGGTGCTG GATGTTGTGA GAAAGGAGGC TGAGAGCTGT GACTGCCTGC AGGGTTTCCA   541 GCTGACCCAC TCCCTGGGTG GGGGGACTGG GTCTGGGATG GGTACCCTCC TCATCAGCAA   601 GATCCGGGAG GAGTACCCAG ACAGGATCAT GAACACGTTT AGTGTGGTGC CTTCGCCCAA   661 AGTGTCAGAC ACAGTGGTGG AGCCCTACAA CGCCACCCTC TCAGTCCACC AGCTCGTAGA   721 AAACACAGAC NANNNCTACT GCATTGATAA CGAAGCTCTC TACGACATTT GCTTCAGAAC   781 CCTAAAGCTG ACCACGCCCA CCTATGGTGA CCTGAACCAC CTGGNGTCTG CTACCATGAG   841 TGGGGTCACC ACCTGCCTGC GCTTNCCAGG CCNGCTCAAT GNTGACCTGC GGAANCTGGC   901 TGTGAACATG GNNCNNNTTC CCCNGNTGNA CTTCTTCNTG CCCGGNTTTG CCCCACTGAN   961 CNNNCNGGGC AGCCAGCANT ACCNGGCGCN NACNGTGNCC GAGCTNNCCC ANNNNANGNT  1021 NGANGCNNNN ACATGANNGC NGNCTGNNNC NCCNNNNNTN NNNNCTNNCT GANNNNNNNN 1081 NN //
Primer: SV40polyA_R2 Region: SV40 pA, insert 3'(SECFP 3') Sequence file: RDB19413_B1Kfb.seq
>D08242A4_B1Kf_3_SV40polyA_R2_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTANCANNN NNNATTGCAT TCATTTTATG    61 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT   121 GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCCGCCGGC CCTGGCCGTG   181 TCGTCGGCCG ACGGCCGGCT GTCGGCCTAT TCGGCCGAAC TGCCGTTCGA CATCCGCGCA   241 TCTCAGTCAG TCAGAGATCT TCCTCCAGAT CCTCCCTTGT ACAGCTCGTC CATGCCGAGA   301 GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG   361 TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG   421 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC GTCCTCGATG   481 TTGTGGCGGA TCTTGAAGTG GGCCTTGATG CCGTTCTTCT GCTTGTCGGC GGTGATATAG   541 ACGTTGTGGC TGATGTAGTT GTACTCTAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG   601 AAGCCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG   661 GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TACGCTCCTG GACGTAGCCT   721 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG   781 CACTGCACGC CCCAGGTCAG GGTGGNCANG ANGGTGGGCC AGGGCACGGG CAGCTTGNCG   841 GTGGTGNAGA TGANNTTCNN GGTCANCTTG CCGTANGTGG NATCGCCCTC GCNNCTCGCC   901 GGANACGCTG AANCTGTGGN NNGNTTACNT CGCCGTCCAG CTCNANNNNN ANGNNNACCA   961 CCCCGGTGAA CNGCTCCNNG CCCTNGCTCN NNNNNGNATN CNANNTCNNN NNCNNATNNN 1021 NNNNNNN //
Primer: EGFP-M_R Region: insert mid(SECFP 5', beta-tubulin 3') Sequence file: RDB19413_B1Kfc.seq
>D08242A4_B1Kf_3_EGFP-M_R_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG NAGTGGGCCT TGATGCCGTT CTTCTGCTTG    61 TCGGCGGTGA TATAGACGTT GTGGCTGATG TAGTTGTACT CTAGCTTGTG CCCCAGGATG   121 TTGCCGTCCT CCTTGAAGCC GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC   181 TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTACGC   241 TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG   301 GGGTAGCGGC TGAAGCACTG CACGCCCCAG GTCAGGGTGG TCACGAGGGT GGGCCAGGGC   361 ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG   421 CCCTCGCCCT CGCCGGACAC GCTGAACCTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC   481 AGGATGGGCA CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGA TCCGAATTCT   541 CCTCCGGATC CTCCGCGGCC GCAGGCCACC TCCTCCTCAG CCTCCTCCTC GAACTCGCCC   601 TCCTCCTCGG CTGTGGCATC CTGGTACTGC TGGTACTCGG ACACCAGGTC ATTCATGTTG   661 CTCTCGGCCT CGGTGAACTC CATCTCGTCC ATGCCCTCGC CCGTGTACCA GTGCAGGAAG   721 GCCTTGCGCC GGAACATGGC CGTGAACTGC TCGGAGATGC GCTTGAACAG CTCCTGGATG   781 GCCGTGCTGT TGCCNATGAA NGTGGCGGAC ATTTTTAGCC NNCGAGGTGG GANGTCACAG   841 ACAGCCGTTT TCNCATTGTT GGGGATCCAC TCAACAAAAT ANCTGCTGNT TTTGTTTTGN   901 ANATTAAGCA TTTGCTCNTN NNCCTNCTTC ATGGACATGC NGCCCCTGAA NNCNGCGGCN   961 ACCGNCNNNA NCNNCCATNG NGGGGGNCNN NNNNGCCATC ATGNNCNNNG NNTNNAACAT 1021 CNGCTNGGNG ANNNNNGNNN NNNNCANCNN CNNGNTNNNN NNNGNNNNNN NNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB19413_B1Kfd.seq
>D08242A4_B1Kf_3_SV40pro_ori_F_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CGGCCTCTGA GCTNTTCNNG AAGTAGTGAG    61 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG   121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   301 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG   661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT   781 CAGGACNTAG CGTTGGCTAC CCGTGATATT GCTGANNAGC TTGNCGGCGA ATGGGCTGAC   841 CGCTTCCTCG NGCTTTNCGG NATCNCCGCT NCCGATTCNN NNCGCATCGC CTTCTATCGC   901 CTTCNTGACN ANNTNNTTCT GANCGGGANT CTGGGGTTCG AAATGACCGA NCAANCGANG   961 CCCANCNTGN CNTCNCNANA TTTCNATTCC NCNGCCNNCN TCNATGAANN NNGGGCTNCN 1021 NANNCNNTTT NNNGNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.27

GNP_filter3_RDBDEP_html_220727.pl