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pFX-beta-tubulin-SECFP

Expression vector of SECFP with microtuble-targeting sequence (human TUBB4B) in mammalian cells.

Catalog number RDB19413
Resource name pFX-beta-tubulin-SECFP
Clone info. Expression vector of SECFP (super-enhanced cyan-emitting fluorescent protein) with microtuble-targeting sequence (human tubulin beta 4B class IVb; TUBB4B) in mammalian cells.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pFX (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human TUBB4B cDNA
Depositor|Developer Ohba, Yusuke |
Other clones in our bank

External Database
human TUBB4B

          Reference sequence
            
           
          Sequence (full) RDB19413zzk02.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)。
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          RDB19413 pFX-beta-tubulin-SECFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Kashiwagi, S., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualization of Organelles (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19413_B1Kfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, insert 5'(beta-tubulin 5')
          Sequence file: RDB19413_B1Kfa.seq check
          >D08242A4_B1Kf_3_CMV_Forward_A02_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNC NAATGGGCGG TAGGCNNTGT ACGGTGGGAG
             61 GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTTCTAG
            121 AGCCATGCTC GAGACCACCA TGAGGGAAAT CGTGCACTTG CAGGCCGGGC AGTGCGGCAA
            181 CCAAATCGGC GCCAAGTTTT GGGAGGTGAT CAGCGATGAG CACGGCATCG ACCCCACGGG
            241 CACCTACCAC GGGGACAGCG ACCTGCAGCT GGAACGCATC AACGTGTACT ACAATGAGGC
            301 CACCGGCGGC AAGTACGTGC CCCGCGCCGT GCTCGTGGAT CTGGAGCCCG GCACCATGGA
            361 CTCCGTGCGC TCGGGGCCCT TCGGGCAGAT CTTCCGGCCG GACAACTTCG TTTTCGGTCA
            421 GAGTGGTGCT GGGAACAACT GGGCCAAGGG GCACTACACA GAAGGCGCGG AGCTGGTGGA
            481 CTCGGTGCTG GATGTTGTGA GAAAGGAGGC TGAGAGCTGT GACTGCCTGC AGGGTTTCCA
            541 GCTGACCCAC TCCCTGGGTG GGGGGACTGG GTCTGGGATG GGTACCCTCC TCATCAGCAA
            601 GATCCGGGAG GAGTACCCAG ACAGGATCAT GAACACGTTT AGTGTGGTGC CTTCGCCCAA
            661 AGTGTCAGAC ACAGTGGTGG AGCCCTACAA CGCCACCCTC TCAGTCCACC AGCTCGTAGA
            721 AAACACAGAC NANNNCTACT GCATTGATAA CGAAGCTCTC TACGACATTT GCTTCAGAAC
            781 CCTAAAGCTG ACCACGCCCA CCTATGGTGA CCTGAACCAC CTGGNGTCTG CTACCATGAG
            841 TGGGGTCACC ACCTGCCTGC GCTTNCCAGG CCNGCTCAAT GNTGACCTGC GGAANCTGGC
            901 TGTGAACATG GNNCNNNTTC CCCNGNTGNA CTTCTTCNTG CCCGGNTTTG CCCCACTGAN
            961 CNNNCNGGGC AGCCAGCANT ACCNGGCGCN NACNGTGNCC GAGCTNNCCC ANNNNANGNT
           1021 NGANGCNNNN ACATGANNGC NGNCTGNNNC NCCNNNNNTN NNNNCTNNCT GANNNNNNNN
          1081 NN
          //
          Primer: SV40polyA_R2
          Region: SV40 pA, insert 3'(SECFP 3')
          Sequence file: RDB19413_B1Kfb.seq check
          >D08242A4_B1Kf_3_SV40polyA_R2_B02_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTANCANNN NNNATTGCAT TCATTTTATG
             61 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
            121 GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCCGCCGGC CCTGGCCGTG
            181 TCGTCGGCCG ACGGCCGGCT GTCGGCCTAT TCGGCCGAAC TGCCGTTCGA CATCCGCGCA
            241 TCTCAGTCAG TCAGAGATCT TCCTCCAGAT CCTCCCTTGT ACAGCTCGTC CATGCCGAGA
            301 GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG
            361 TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG
            421 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC GTCCTCGATG
            481 TTGTGGCGGA TCTTGAAGTG GGCCTTGATG CCGTTCTTCT GCTTGTCGGC GGTGATATAG
            541 ACGTTGTGGC TGATGTAGTT GTACTCTAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG
            601 AAGCCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG
            661 GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TACGCTCCTG GACGTAGCCT
            721 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG
            781 CACTGCACGC CCCAGGTCAG GGTGGNCANG ANGGTGGGCC AGGGCACGGG CAGCTTGNCG
            841 GTGGTGNAGA TGANNTTCNN GGTCANCTTG CCGTANGTGG NATCGCCCTC GCNNCTCGCC
            901 GGANACGCTG AANCTGTGGN NNGNTTACNT CGCCGTCCAG CTCNANNNNN ANGNNNACCA
            961 CCCCGGTGAA CNGCTCCNNG CCCTNGCTCN NNNNNGNATN CNANNTCNNN NNCNNATNNN
          1021 NNNNNNN
          //
          Primer: EGFP-M_R
          Region: insert mid(SECFP 5', beta-tubulin 3')
          Sequence file: RDB19413_B1Kfc.seq check
          >D08242A4_B1Kf_3_EGFP-M_R_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG NAGTGGGCCT TGATGCCGTT CTTCTGCTTG
             61 TCGGCGGTGA TATAGACGTT GTGGCTGATG TAGTTGTACT CTAGCTTGTG CCCCAGGATG
            121 TTGCCGTCCT CCTTGAAGCC GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC
            181 TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTACGC
            241 TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG
            301 GGGTAGCGGC TGAAGCACTG CACGCCCCAG GTCAGGGTGG TCACGAGGGT GGGCCAGGGC
            361 ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG
            421 CCCTCGCCCT CGCCGGACAC GCTGAACCTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC
            481 AGGATGGGCA CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGA TCCGAATTCT
            541 CCTCCGGATC CTCCGCGGCC GCAGGCCACC TCCTCCTCAG CCTCCTCCTC GAACTCGCCC
            601 TCCTCCTCGG CTGTGGCATC CTGGTACTGC TGGTACTCGG ACACCAGGTC ATTCATGTTG
            661 CTCTCGGCCT CGGTGAACTC CATCTCGTCC ATGCCCTCGC CCGTGTACCA GTGCAGGAAG
            721 GCCTTGCGCC GGAACATGGC CGTGAACTGC TCGGAGATGC GCTTGAACAG CTCCTGGATG
            781 GCCGTGCTGT TGCCNATGAA NGTGGCGGAC ATTTTTAGCC NNCGAGGTGG GANGTCACAG
            841 ACAGCCGTTT TCNCATTGTT GGGGATCCAC TCAACAAAAT ANCTGCTGNT TTTGTTTTGN
            901 ANATTAAGCA TTTGCTCNTN NNCCTNCTTC ATGGACATGC NGCCCCTGAA NNCNGCGGCN
            961 ACCGNCNNNA NCNNCCATNG NGGGGGNCNN NNNNGCCATC ATGNNCNNNG NNTNNAACAT
          1021 CNGCTNGGNG ANNNNNGNNN NNNNCANCNN CNNGNTNNNN NNNGNNNNNN NNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, NeoR_KanR
          Sequence file: RDB19413_B1Kfd.seq check
          >D08242A4_B1Kf_3_SV40pro_ori_F_D02_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CGGCCTCTGA GCTNTTCNNG AAGTAGTGAG
             61 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG
            121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            301 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
            661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
            781 CAGGACNTAG CGTTGGCTAC CCGTGATATT GCTGANNAGC TTGNCGGCGA ATGGGCTGAC
            841 CGCTTCCTCG NGCTTTNCGG NATCNCCGCT NCCGATTCNN NNCGCATCGC CTTCTATCGC
            901 CTTCNTGACN ANNTNNTTCT GANCGGGANT CTGGGGTTCG AAATGACCGA NCAANCGANG
            961 CCCANCNTGN CNTCNCNANA TTTCNATTCC NCNGCCNNCN TCNATGAANN NNGGGCTNCN
          1021 NANNCNNTTT NNNGNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kashiwagi, S., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740.

          2022.07.27

          GNP_filter3_RDBDEP_html_220727.pl