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pFX-GalT-SECFP

Expression vector of SECFP with Golgi-targeting sequence (human B4GALT1) in mammalian cells.

Catalog number RDB19412
Resource name pFX-GalT-SECFP
Clone info. Expression vector of SECFP (super-enhanced cyan-emitting fluorescent protein) with Golgi-targeting sequence (human beta-1,4-galactosyltransferase 1; B4GALT1/GalT) in mammalian cells.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pFX (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human B4GALT1 cDNA
Depositor|Developer Ohba, Yusuke |
 
Sequence (full) RDB19412hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19412_B1Kfp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)。
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RDB19412 pFX-GalT-SECFP DNA solution


How to cite this biological resource

Materials & Methods section:

The pFX-GalT-SECFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19412).

Reference section:

Kashiwagi, S., Fujioka, Y., Satoh, A.O., Yoshida, A., Fujioka, M., Nepal, P., Tsuzuki, A., Aoki, O., Paudel, S., Sasajima, H., Ohba, Y., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Kashiwagi, S., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740. [link to RRC of NBRP]