Resource data sheet
Prev.
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pFX-SECFP-Sec61 beta

Expression vector of SECFP with ER-targeting sequence (human SEC61B) in mammalian cells.

Catalog number RDB19411
Resource name pFX-SECFP-Sec61 beta
Clone info. Expression vector of SECFP (super-enhanced cyan-emitting fluorescent protein) with ER-targeting sequence (human SEC61 translocon subunit beta; SEC61B/Sec61 beta) in mammalian cells.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pFX (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human SEC61B cDNA
Depositor|Developer Ohba, Yusuke |
Other clones in our bank
External Database
human SEC61B
          Reference sequence
            
           
          Sequence (full) RDB19411hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19411_B1Kfp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Please visit Information of Request for Distribution.[link] 
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19411 pFX-SECFP-Sec61 beta DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The pFX-SECFP-Sec61 beta was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19411).

          Reference section:

          Kashiwagi, S., Fujioka, Y., Satoh, A.O., Yoshida, A., Fujioka, M., Nepal, P., Tsuzuki, A., Aoki, O., Paudel, S., Sasajima, H., Ohba, Y., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          Featured content Visualization of Organelles (English text)

          References

          Original, user report and related articles

          original Kashiwagi, S., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740. [link to RRC of NBRP]

          2023.07.02

          GNP_filter3_RDBDEP_html_230702.pl