Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFX-SECFP-Sec61 beta

Expression vector of SECFP with ER-targeting sequence (human SEC61B) in mammalian cells.

Sequence (full)	RDB19411zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19411_B1Kfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,SECFP 5' Sequence file: RDB19411_B1Kfa.seq
>19411_19411_B1Kf_2_CMV_Forward_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGGCGG TAGGCGTGTA CGGTGGGAGG    61 TCTATATAAG CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTTCTAGA   121 GCCATGGAAT TCGGATCCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC   181 ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAGGT TCAGCGTGTC CGGCGAGGGC   241 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG   301 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTGGG GCGTGCAGTG CTTCAGCCGC   361 TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC   421 CAGGAGCGTA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG   481 TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGGCTT CAAGGAGGAC   541 GGCAACATCC TGGGGCACAA GCTAGAGTAC AACTACATCA GCCACAACGT CTATATCACC   601 GCCGACAAGC AGAAGAACGG CATCAAGGCC CACTTCAAGA TCCGCCACAA CATCGAGGAC   661 GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG   721 CTGCTGCCCG ACAACCACTA CCTGANCACC CAGTCCGCCC TGAGCAAAGA CCCCNACGAG   781 AANCGCGATC ACATGGTCCT GCTGGAGTTC GTGANNGCCG CCGGGNTCAC TCTCGGCATG   841 GACGANCTGT ACAAGGNANG NTCTNGANGA AGATCTGGNA NGATCCNGAN GANTCNNNCC   901 CACCATGCNT GGTCCGNNCC CCCANNGNCN NTAACGNGNN ATCCNCANGN CGCTCTCCCA   961 GCANNCAGNN GNNNNNNGGG CNNCNNNTNC NNNNNNCNGG CNNNNGAAAA NNGNNNNNTN 1021 NGGGNNNNNN NNNN //
Primer: SV40polyA_R2 Region: insert, SECFP3' Sequence file: RDB19411_B1Kfb.seq
>19411_19411_B1Kf_2_SV40polyA_R2_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ATTGCATTCN TTTTATGTTT    61 CNGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT   121 ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCTT CAGTCAGTCA GCGGCCGCCC   181 GAACGAGTGT ACTTGCCCCA AATGTGCAAC ATAAATACAG AAGCGATGAA CAGAAGACTC   241 ATAACCAATA CTGGAACAGG GCCAACTTTG AGCCCAGGTG AATCTTCTGT GTAGAATCGC   301 CACATCCCCC CGGTGCCTGC CGAGGTTGTG CGGCCTGCAC TCCTTGTCCC ACAGCTGGCA   361 TTTTTCCTCT GCCGGACAGT GGATCCCGCC GCCCGGGCGG CCACTGCTTT GCTGGGAGAG   421 CGCCCTGAGG ATCCCACGTT AGTGCCACTG GGGGTCGGAC CAGGCATGGT GGGCTCGAGT   481 CCTCCGGATC CTCCAGATCT TCCTCCAGAT CCTCCCTTGT ACAGCTCGTC CATGCCGAGA   541 GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG   601 TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG   661 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC GTCCTCGATG   721 TTGTGGCGGA TCTTGAAGTG GGCCTTGATG CCGTTCTTCT GCTTGTCGGC GGTGATATAG   781 ACGTTGTGGC TGATGTAGTT GTACTCTAGC TTGTGCCCCA NGANGTTGCC GTCCTCCTTG   841 AAGCCGATGC CCTTCAGCTC GATGCNGGTT CACCANGGNG TCGCCCTCNA ACTTCANCTC   901 NNCGCGGGTC NNGNAGTTGC CGTCNNNCTT GAANAANANG GTACNCTNCT NGANGTANCN   961 NTCNGGCATN GCGNANTTGN NNAANTCNTG CTGCTNCNNG NGGNCGGGGT ANNNGNNTGN  1021 NNNNNNGNNN NCCCCNNNNN CNNNNNNGGN NNNNNANNNN NNNNNCNNNG GNNNNGNNNN 1081 NNTNNNNNNN NGNNNNNNNN NN //
Primer: SV40pro_ori_F Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB19411_B1Kfc.seq
>19411_19411_B1Kf_2_SV40pro_ori_F_G05_20_ABI24.ab1     1 NNNNNNNNNC NNNNNGCTAT TCNNGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT    61 TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT   121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC   181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC   241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC   301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG   361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC   421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG   481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC   541 GGATGGAAGC CGGTCTTGTC GATCANGATG ATCTGNACGA AGANCNTNNN NGGGCTCGCG   601 CCNNNNNAAC TGTTCGCCNN GCTCNAGGCN ANNNNNNNCC NACGGCGAGG NTCTCNNCNN   661 NNNCCANGGN NNNNGCCTNN NNGCNNAANA TNANNNNNGN AANNNNNNNC NTTNTCNGNA 721 TNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl