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pFX-SECFP-Sec61 beta

Expression vector of SECFP with ER-targeting sequence (human SEC61B) in mammalian cells.

Catalog number RDB19411
Resource name pFX-SECFP-Sec61 beta
Clone info. Expression vector of SECFP (super-enhanced cyan-emitting fluorescent protein) with ER-targeting sequence (human SEC61 translocon subunit beta; SEC61B/Sec61 beta) in mammalian cells.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pFX (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human SEC61B cDNA
Depositor|Developer Ohba, Yusuke |
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External Database
human SEC61B

          Reference sequence
            
           
          Sequence (full) RDB19411zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)。
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          RDB19411 pFX-SECFP-Sec61 beta DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Kashiwagi, S., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualization of Organelles (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19411_B1Kfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,SECFP 5'
          Sequence file: RDB19411_B1Kfa.seq check
          >19411_19411_B1Kf_2_CMV_Forward_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGGCGG TAGGCGTGTA CGGTGGGAGG
             61 TCTATATAAG CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTTCTAGA
            121 GCCATGGAAT TCGGATCCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC
            181 ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAGGT TCAGCGTGTC CGGCGAGGGC
            241 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG
            301 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTGGG GCGTGCAGTG CTTCAGCCGC
            361 TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC
            421 CAGGAGCGTA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG
            481 TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGGCTT CAAGGAGGAC
            541 GGCAACATCC TGGGGCACAA GCTAGAGTAC AACTACATCA GCCACAACGT CTATATCACC
            601 GCCGACAAGC AGAAGAACGG CATCAAGGCC CACTTCAAGA TCCGCCACAA CATCGAGGAC
            661 GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG
            721 CTGCTGCCCG ACAACCACTA CCTGANCACC CAGTCCGCCC TGAGCAAAGA CCCCNACGAG
            781 AANCGCGATC ACATGGTCCT GCTGGAGTTC GTGANNGCCG CCGGGNTCAC TCTCGGCATG
            841 GACGANCTGT ACAAGGNANG NTCTNGANGA AGATCTGGNA NGATCCNGAN GANTCNNNCC
            901 CACCATGCNT GGTCCGNNCC CCCANNGNCN NTAACGNGNN ATCCNCANGN CGCTCTCCCA
            961 GCANNCAGNN GNNNNNNGGG CNNCNNNTNC NNNNNNCNGG CNNNNGAAAA NNGNNNNNTN
          1021 NGGGNNNNNN NNNN
          //
          Primer: SV40polyA_R2
          Region: insert, SECFP3'
          Sequence file: RDB19411_B1Kfb.seq check
          >19411_19411_B1Kf_2_SV40polyA_R2_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ATTGCATTCN TTTTATGTTT
             61 CNGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
            121 ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCTT CAGTCAGTCA GCGGCCGCCC
            181 GAACGAGTGT ACTTGCCCCA AATGTGCAAC ATAAATACAG AAGCGATGAA CAGAAGACTC
            241 ATAACCAATA CTGGAACAGG GCCAACTTTG AGCCCAGGTG AATCTTCTGT GTAGAATCGC
            301 CACATCCCCC CGGTGCCTGC CGAGGTTGTG CGGCCTGCAC TCCTTGTCCC ACAGCTGGCA
            361 TTTTTCCTCT GCCGGACAGT GGATCCCGCC GCCCGGGCGG CCACTGCTTT GCTGGGAGAG
            421 CGCCCTGAGG ATCCCACGTT AGTGCCACTG GGGGTCGGAC CAGGCATGGT GGGCTCGAGT
            481 CCTCCGGATC CTCCAGATCT TCCTCCAGAT CCTCCCTTGT ACAGCTCGTC CATGCCGAGA
            541 GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG
            601 TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG
            661 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC GTCCTCGATG
            721 TTGTGGCGGA TCTTGAAGTG GGCCTTGATG CCGTTCTTCT GCTTGTCGGC GGTGATATAG
            781 ACGTTGTGGC TGATGTAGTT GTACTCTAGC TTGTGCCCCA NGANGTTGCC GTCCTCCTTG
            841 AAGCCGATGC CCTTCAGCTC GATGCNGGTT CACCANGGNG TCGCCCTCNA ACTTCANCTC
            901 NNCGCGGGTC NNGNAGTTGC CGTCNNNCTT GAANAANANG GTACNCTNCT NGANGTANCN
            961 NTCNGGCATN GCGNANTTGN NNAANTCNTG CTGCTNCNNG NGGNCGGGGT ANNNGNNTGN
           1021 NNNNNNGNNN NCCCCNNNNN CNNNNNNGGN NNNNNANNNN NNNNNCNNNG GNNNNGNNNN
          1081 NNTNNNNNNN NGNNNNNNNN NN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR_KanR
          Sequence file: RDB19411_B1Kfc.seq check
          >19411_19411_B1Kf_2_SV40pro_ori_F_G05_20_ABI24.ab1
              1 NNNNNNNNNC NNNNNGCTAT TCNNGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
             61 TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
            121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
            181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
            241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC
            301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
            361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
            421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
            481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
            541 GGATGGAAGC CGGTCTTGTC GATCANGATG ATCTGNACGA AGANCNTNNN NGGGCTCGCG
            601 CCNNNNNAAC TGTTCGCCNN GCTCNAGGCN ANNNNNNNCC NACGGCGAGG NTCTCNNCNN
            661 NNNCCANGGN NNNNGCCTNN NNGCNNAANA TNANNNNNGN AANNNNNNNC NTTNTCNGNA
          721 TNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kashiwagi, S., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740.

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl