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pFX-Tom20-SECFP

Expression vector of SECFP with mitochondria-targeting sequence (human TOMM20) in mammalian cells.

Catalog number RDB19410
Resource name pFX-Tom20-SECFP
Clone info. Expression vector of SECFP (super-enhanced cyan-emitting fluorescent protein) with mitochondria-targeting sequence (human translocase of outer mitochondrial membrane 20; TOMM20/TOM20) in mammalian cells.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pFX (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human TOMM20 cDNA
Depositor|Developer Ohba, Yusuke |
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External Database
human TOMM20
          Reference sequence
            
           
          Sequence (full) RDB19410hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19410_B1Kfp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)。
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          RDB19410 pFX-Tom20-SECFP DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The pFX-Tom20-SECFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19410).

          Reference section:

          Kashiwagi, S., Fujioka, Y., Satoh, A.O., Yoshida, A., Fujioka, M., Nepal, P., Tsuzuki, A., Aoki, O., Paudel, S., Sasajima, H., Ohba, Y., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Kashiwagi, S., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740. [link to RRC of NBRP]

          2023.07.02

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