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pEGFPC3_EGFP-hZFAT

Expression vector of human ZFAT tagged with EGFP.

Catalog number RDB19326
Resource name pEGFPC3_EGFP-hZFAT
Clone info. Expression vector of human zinc finger and AT-hook domain containing (ZFAT) tagged with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pEGFP-C3 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human ZFAT cDNA
Depositor|Developer Shirasawa, Senji | Ishikura, Shuhei |
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External Database
human ZFAT

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Ishikura, S. et al., Nucleic Acids Res. 48 (19): 10848-10866, 2020) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Ishikura, S. et al., Nucleic Acids Res. 48 (19): 10848-10866, 2020)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行うこと。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19326 pEGFPC3_EGFP-hZFAT Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Ishikura, S., ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis. Nucleic Acids Res. 48 (19): 10848-10866 (2020). PMID 32997115.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Ishikura, S., ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis. Nucleic Acids Res. 48 (19): 10848-10866 (2020). PMID 32997115.

          2022.07.13

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