Resource data sheet
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AxCBCas9 (#RDB19290)

Recombinant adenovirus expressing spCas9 under the control of CB promoter.

Clone info. Recombinant adenovirus expressing spCas9 (Streptococcus pyogenes Cas9) under the control of CB promoter (consists of the cytomegalo virus enhancer and the chicken beta actin promoter). For more information, see the references of Nakanishi, T. et al. Sci. Rep. 11 (1): 3961, 2021.
Comment This virus does not become high titer even if it is kept low titer at the time of passage, so please culture it at the high titer of stock in the passage.
Vector backbone pAxcwit2 (Adenovirus)
Selectable markers no genetic markers
Growth remarks This virus does not become high titer even if it is kept low titer at the time of passage, so please culture it at the high titer of stock in the passage.
Gene/insert name Streptococcus pyogenes Cas9
Depositor|Developer Nakanishi, Tomoko |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The user uses this adenovirus vector in collaborative basis within three years (up to the year 2024). After the year 2025, the user acknowledges to the depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Nakanishi , T. et al. Sci. Rep. 11 (1): 3961, 2021) No recipient shall use the research material, their progeny or derivatives, commercially without TODAI TLO's prior written approval. (TODAI TLO, Ltd. 3rd Fl., Sangakurenkei-Plaza, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. FAX:+81-3-5805-7699, shigeta@todaitlo.jp)
Remarks
Please obtain written approval of the depositor.
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
遺伝子組換え生物の受入れ確認書 [Word]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、事前に寄託者の提供承認を得る。本リソースを2024年（寄託後3年）以内に利用する時は、寄託者と利用者の共同研究形式をとる。2025年以降の利用は、謝辞の表明を必要とする。利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Nakanishi , T. et al. Sci. Rep. 11 (1): 3961, 2021)。利用者が本件リソースを商業利用する場合は、利用者は株式会社 東京大学ＴＬＯ（〒165-0035 東京都文京区本郷７－３－１産学連携プラザ３階　FAX:03-5805-7699　shigeta@todaitlo.jp）の事前承諾を得るものとする。　
備考
組換え体提供にかかる書式が必要です。
寄託者の書面による提供承諾が必要です。
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19290 AxCBCas9 Under QC test. Please contact us. Virus, lysate of infected cell culture JPY 32,340 plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The AxCBCas9 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19290).

Reference section:

Nakanishi, T., Maekawa, A., Suzuki, M., Tabata, H., Sato, K., Mori, M., Saito, I., Construction of adenovirus vectors simultaneously expressing four multiplex, double-nicking guide RNAs of CRISPR/Cas9 and in vivo genome editing. Sci. Rep. 11 (1): 3961 (2021). PMID 33597562. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nakanishi, T., Construction of adenovirus vectors simultaneously expressing four multiplex, double-nicking guide RNAs of CRISPR/Cas9 and in vivo genome editing. Sci. Rep. 11 (1): 3961 (2021). PMID 33597562. [link to RRC of NBRP]