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pcDNA3.1 3xFLAG::LC3B G120A (#RDB19197)

Expression vectors of human ATG8 homolog LC3B lipidation-defective mutant (G120A) tagged with 3xFLAG at N-terminus.

Clone info. Expression vectors of human ATG8 homolog LC3B lipidation-defective mutant (G120A) tagged with 3xFLAG at N-terminus.
Comment Expression was confirmed by the depositor with co-immunoprecipitation.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human MAP1LC3B cDNA
Depositor|Developer Yoshimori, Tamotsu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nakamura, S. et al., Nat. Cell Biol., 22 (10): 1252-1263, 2020). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a separate material transfer agreement with Osaka University.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakamura, S. et al., Nat. Cell Biol., 22 (10): 1252-1263, 2020)。利用者が非営利目的の研究・教育以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19197 pcDNA3.1 3xFLAG::LC3B G120A Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1 3xFLAG::LC3B G120A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19197).

Reference section:

Nakamura, S., Shigeyama, S., Minami, S., Shima, T., Akayama, S., Matsuda, T., Esposito, A., Napolitano, G., Kuma, A., Namba-Hamano, T., Nakamura, J., Yamamoto, K., Sasai, M., Tokumura, A., Miyamoto, M., Oe, Y., Fujita, T., Terawaki, S., Takahashi, A., Hamasaki, M., Yamamoto, M., Okada, Y., Komatsu, M., Nagai, T., Takabatake, Y., Xu, H., Isaka, Y., Ballabio, A., Yoshimori, T., LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury. Nat. Cell Biol. 22 (10): 1252-1263 (2020). PMID 32989250. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nakamura, S., LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury. Nat. Cell Biol. 22 (10): 1252-1263 (2020). PMID 32989250. [link to RRC of NBRP]