Expression vectors of human ATG8 homolog LC3B lipidation-defective mutant (G120A) tagged with 3xFLAG at N-terminus.
Clone info. | Expression vectors of human ATG8 homolog LC3B lipidation-defective mutant (G120A) tagged with 3xFLAG at N-terminus. |
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Comment | Expression was confirmed by the depositor with co-immunoprecipitation. |
Vector backbone | pcDNA3.1 (plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Ampicillin (E. coli), Neomycin (mammalian cell) |
Gene/insert name | human MAP1LC3B cDNA |
Depositor|Developer | Yoshimori, Tamotsu | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nakamura, S. et al., Nat. Cell Biol., 22 (10): 1252-1263, 2020). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a separate material transfer agreement with Osaka University. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakamura, S. et al., Nat. Cell Biol., 22 (10): 1252-1263, 2020)。利用者が非営利目的の研究・教育以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB19197 | pcDNA3.1 3xFLAG::LC3B G120A | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pcDNA3.1 3xFLAG::LC3B G120A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19197). |
Reference section:
Nakamura, S., Shigeyama, S., Minami, S., Shima, T., Akayama, S., Matsuda, T., Esposito, A., Napolitano, G., Kuma, A., Namba-Hamano, T., Nakamura, J., Yamamoto, K., Sasai, M., Tokumura, A., Miyamoto, M., Oe, Y., Fujita, T., Terawaki, S., Takahashi, A., Hamasaki, M., Yamamoto, M., Okada, Y., Komatsu, M., Nagai, T., Takabatake, Y., Xu, H., Isaka, Y., Ballabio, A., Yoshimori, T., LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury. Nat. Cell Biol. 22 (10): 1252-1263 (2020). PMID 32989250. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Nakamura, S., LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury. Nat. Cell Biol. 22 (10): 1252-1263 (2020). PMID 32989250. [link to RRC of NBRP] |
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