Resource data sheet
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pCAGGS-CXCL12-mCherry (#RDB19182)

Expression vector of mouse Cxcl12.


Drawn by SnapGene® software
Sequence (full) RDB19182hts01.seq
Publication Zhu, Y., Sci. Rep. 10 (1): 11830 (2020). [link to RRC of NBRP]
Test sheet RDB19182_B1G1p1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of mouse chemokine (C-X-C motif) ligand 12 (Cxcl12) tagged with mCherry at C-terminus.
Comment Expression was confirmed by the depositor via transfection into COS7 cells and detection of mChery signal in conditional medium
Vector backbone pCAGGS with MCS (pllasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Cxcl12 cDNA
Depositor|Developer Zhu, Yan |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Zhu, Y. et al., Sci. Rep., 10 (1): 11830, 2020).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Zhu, Y. et al., Sci. Rep., 10 (1): 11830, 2020).
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19182 pCAGGS-CXCL12-mCherry DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAGGS-CXCL12-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19182).

Reference section:

Zhu, Y., Hirata, T., Mackay, F., Murakami, F., Chemokine receptor CXCR7 non-cell-autonomously controls pontine neuronal migration and nucleus formation. Sci. Rep. 10 (1): 11830 (2020). PMID 32678266. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Zhu, Y., Chemokine receptor CXCR7 non-cell-autonomously controls pontine neuronal migration and nucleus formation. Sci. Rep. 10 (1): 11830 (2020). PMID 32678266. [link to RRC of NBRP]