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pBS-CaMKIIpro-G-CaMP9a-WPRE

Expression vector of genetically encoded calcium indicator, G-CaMP9a.

Catalog number RDB19172
Resource name pBS-CaMKIIpro-G-CaMP9a-WPRE
Clone info. Expression vector of genetically encoded calcium indicator, G-CaMP9a.
Comment Expression was confirmed by the depositor with transfection to cultured cells.
Vector backbone pBluescript II (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Camk2a genomic rat Calm2 cDNA; Aequorea victoria GFP (cpGFP) cDNA
Reference of insert sequence LC654935 (DDBJ accession number)
Depositor|Developer Bito, Haruhiko | Sakamoto, Masayuki | Inoue, Masatoshi |
 
Sequence (full) RDB19172hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19172_B4Djp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sakamoto, M., et al., Cell Reports Methods 2: 100168 (2022)) is requested.
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Sakamoto, M., et al., Cell Reports Methods 2: 100168 (2022)) の引用を必要とする。
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RDB19172 pBS-CaMKIIpro-G-CaMP9a-WPRE DNA solution


How to cite this biological resource

Materials & Methods section:

The pBS-CaMKIIpro-G-CaMP9a-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19172).

Reference section:

Sakamoto, M., Inoue, M., Takeuchi, A., Kobari, S., Yokoyama, T., Horigane, S., Takemoto-Kimura, S., Abe, M., Sakimura, K., Kano, M., Kitamura, K., Fujii, H., Bito, H., A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Reports Methods 2: 100168 (2022). PMID 35474964. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Sakamoto, M., A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Reports Methods 2: 100168 (2022). PMID 35474964. [link to RRC of NBRP]