Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBS-CaMKIIpro-G-CaMP9a-WPRE

Expression vector of genetically encoded calcium indicator, G-CaMP9a.

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sakamoto, M., et al., Cell Reports Methods 2: 100168 (2022)) is requested.
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Sakamoto, M., et al., Cell Reports Methods 2: 100168 (2022)) の引用を必要とする。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19172_B1Kup1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert 5'(mouse CaMKII pro) Sequence file: RDB19172_B1Kuf.seq
>D08506A1_B1Ku_1_pUC-M13_-100_B03_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CCNGGGTTTT CCCNGTCACG ACGTTGTAAA    61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCACCGC   121 GGTGGCGGCC GCATGCATCA TTATGGCCTT AGGTCACTTC ATCTCCATGG GGTTCTTCTT   181 CTGATTTTCT AGAAAATGAG ATGGGGGTGC AGAGAGCTTC CTCAGTGACC TGCCCAGGGT   241 CACATCAGAA ATGTCAGAGC TAGAACTTGA ACTCAGATTA CTAATCTTAA ATTCCATGCC   301 TTGGGGGCAT GCAAGTACGA TATACAGAAG GAGTGAACTC ATTAGGGCAG ATGACCAATG   361 AGTTTAGGAA AGAAGAGTCC AGGGCAGGGT ACATCTACAC CACCCGCCCA GCCCTGGGTG   421 AGTCCAGCCA CGTTCACCTC ATTATAGTTG CCTCTCTCCA GTCCTACCTT GACGGGAAGC   481 ACAAGCAGAA ACTGGGACAG GAGCCCCAGG AGACCAAATC TTCATGGTCC CTCTGGGAGG   541 ATGGGTGGGG AGAGCTGTGG CAGAGGCCTC AGGAGGGGCC CTGCTGCTCA GTGGTGACAG   601 ATAGGGGTGA GAAAGCAGAC AGAGTCATTC CGTCAGCATT CTGGGTCTGT TTGGTACTTC   661 TTCTCACGCT AAGGTGGCGG TGTGATATGC ACAATGGCTA AAAAGCAGGG AGAGCTGGAA   721 AGAAACAAGG ACAGAGACAG AGGCCAAGTC AACCAGACCA ATTCCCAGAG GAAGCAAAGA   781 AACCATTACA GANACTACNA GGGGGANNGG AAGGAGAGAT GANTTANCTT CCCCTGTAAA   841 CCTTANAACC CANCTGTTGC NAGGGNNACG GGGCAATACC TGTCTCTTCA NANGANATGA   901 AGTTGCCAGG GTANNTACNT CCTGTCTTNC TCANGGANCA TCCCNNAATG TNNNACCCAC   961 TNNCCGTTAC CNNANCAACT GNCNTNTTTG CCCCNNTTNA NCCCATCCCG TCNNNNAAAN 1021 NNNCCCTANN NNTNNNNNNN NGGGGNNNNN NNNNANNNNN NNNNN //
Primer: SV40_F2 (Pr1194) Region: SV40 pA,lac pro,T3 pro Sequence file: RDB19172_B1Kug.seq
>D08506A1_B1Ku_1_SV40_F2_F03_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTNNNGT CTTCGCCTTC GCCCTCNAGA NGAGTCGGAT    61 CTCCCTTTGG GNCGNNTCCC CGCATCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA   121 TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA   181 TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA   241 ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA   301 AGTAAAACCT CTACAAATGT GGTAAAATCG ATAAGGATCC AACTCGAGCT ACTAGTAGGG   361 TACCAGGAAT TCGTTTCGAA GCGTCGACTC TCCGGATTAA TTAAGTACCC AGCTTTTGTT   421 CCCTTTAGTG AGGGTTAATT GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT   481 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGGAGCATA AAGTGTAAAG   541 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT   601 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG   661 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG   721 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT   781 CAGGGGATAA CGCAGGAAAG AACATGTGAG CNAAAAGGNC AGCAAAAGGC CAGGAACCGT   841 AAAAANGNCG CGTTGCTGGC GTTTTNCCNT ANGCTCCGCC CCCCCTGANN ANCATCACAA   901 AANTCGNNGC TCAAGTCANA NGTGGNGAAN NCCGNNNNNA NNNNNAAGNT ANCANGCGTT 961 TCCCCNNNGA ANCTCCCTNN NGCNNTCTNC NGNTNCCNAN NNNNNNNNN //
Primer: EGFP-C-rev (Pr0026) Region: insert mid(GCaMP9a 5',mouse CaMKII pro) Sequence file: RDB19172_B1Kuh.seq
>D08506A1_B1Ku_1_EGFP-C-rev_D03_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNNTGGGT GCTCAGGTAG TGGTTGTCGG    61 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTAG GCGAGCTGCA   121 CGCCGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCGC CTTGATGCCG TTCTTCTGCT   181 TGTCGGCCAT GATATAGACG TTCTCGAGAA ATGGGTTTCC AAAGGAACGC TTTCTCAGCA   241 TAGACTTGAC CAGGATCACA GTGGTCCAGC TGGGGATGAG CTTGACGTCG ACCATGGTGG   301 CGAGATCCTT ATCGTCATCG TCGTACAGAT CCCGACCCAT TTGCTGTCCA CCAGTCATGC   361 TAGCCATACC ATGATGATGA TGATGATGAG AACCCATGGT GGCACCGGTA GCGCTGCCCC   421 CAGAACTAGG GGCCACTCGC CTGCCCGTGC TCCTGAGTGC AAACGGAGAA CCGGCTTGAC   481 TGACGAGCTT GGGGCTTCTG AGCAGGGCAC TGTGGCTGCT CACAGCCTCG CGAGCCTTCG   541 TCAGCATCCA GGTCCCCATG GCAACCACCT CCGAAACGCC CTGTTCCCGT TGCCCCGGTA   601 ACTGCCTCCC CAACACCTGC CTGCCTTTCA CTTTAAAACC TGCTCCTCTT TGCCCTGGCC   661 TTATATACAC AATACTGCAG GACTGTGTGG GCCCAGGAGC AAGTGGACCC TGTTCCCCCA   721 GGGTGATGTA CTGAGGGGGT TGGAGAAGGG GATGCGAACA AACTTAGTCC ACAAGTGATC   781 ATCGCTCTTC CTACCTCCCC CACCTCNNAN CTATAGGGCC CTTTTNACAG ACNGGATGGG   841 ATTAAGTGGG GCAAAGAGGC AGTTGNTATG GTAACGGCTA GTGGGTGCCA CNTTCTGGGN   901 NGGNNCTTGA NAAANANAGG ANGTANTTAC CNTGNCANCN TCATCTCCNN TGAANANNCN   961 NNNANTGCCC CNTTGCCCNN NNNNNNGCTG GNNNCTNNNN TTNCANGGGN NNNNNNANNN 1021 NNNNNNN //
Primer: PRE_rev (Pr0250) Region: insert mid(WPRE,GCaMP9a 3') Sequence file: RDB19172_B1Kui.seq
>D08506A1_B1Ku_1_PRE_rev_C03_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGGAGCA ACATANNNNN NAATACCAGT CAATCTTTCA    61 CAAATTTTGT AATCCANANG TTGATTATCT AGTACGCGTT CACTTCGCTG TCATCATTTG   121 TACAAACTCT TCGTAGTTTA CCTGACCATC CCCATCGATG TCTGCTTCCC TGATCATTTC   181 ATCAACCTCT TCATCTGTTA ACTTCTCTCC AAGGTTTGTC ATCACGTGGC GAAGCTCTGC   241 TGCACTGATG TAGCCATTGC CATCCTTATC AAACACACGG AACGCTTCTC TAATTTCTTC   301 TTCACTGTCT GTGTCTTTCA TTTTTCTTGC CATCATTGTC AGGAACTCAG GGAAGTCGAT   361 TGTGCCATTA CCGTCGGCAT CTACTTCATT GATCATGTCC TGCAGCTCTG CTTCTGTGGG   421 GTTCTGCCCC AGAGACCGCA GCACCGTCCC CAGCTCCTTG GTTGTTATTG TCCCATCCCC   481 GTCCTTGTCA AATAGGGAGA AAGCCTCTTT AAATTCTGCG ATCTGCTCTT CAGTCAGTTG   541 GTCCGTGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC   601 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT   661 GTAGGTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ATGTAGCCTT CGGGCATGGC   721 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC   781 GTANGTCANG GTGGTCACGA NGGTGGGCCA GGGCACGGGC AGCTTGCCNG TGGTGCAGAT   841 GANCTTCNNG GTCAGCTTGC CGTTGGTGGC ATCGCCCTCA CCCTCGCCGC GCACGCTGAA   901 CTTGTNNCCG TTNACGTCNC NNNCCAGCTC GANCAGGANG GGNACCACCC CNGNNANNAN   961 CTNCNTCNNC CTTGCTCACN NTGNTCCNNN CNGNANNNCC CNTNGTANNN CNNGNCNATN 1021 NNGANANTGA TNCNNNNNNN CNNNNANNNN NNNNNN //
Primer: ECFP-C_F1 (Pr0925) Region: insert mid(GCaMP9a mid) Sequence file: RDB19172_B1Kuj.seq
>D08506A1_B1Ku_1_ECFP-C_F1_E03_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CGNNCNCNTN NNNCCTGCTG GGAGTTCGTG    61 ACCGCCGCCG GGNTCACTCT CGGCATGGAC GAGCTGTACA AGGGCGGTAC CGGAGGGAGC   121 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC   181 GGCGACGTAA ACGGCCACAA GTTCAGCGTG CGCGGCGAGG GTGAGGGCGA TGCCACCAAC   241 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC   301 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG   361 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACA TCCAGGAGCG CACCATCTTC   421 TTCAAGGACG ACGGCACCTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG   481 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC   541 AAGCTGGAGT ACAACACGGA CCAACTGACT GAAGAGCAGA TCGCAGAATT TAAAGAGGCT   601 TTCTCCCTAT TTGACAAGGA CGGGGATGGG ACAATAACAA CCAAGGAGCT GGGGACGGTG   661 CTGCGGTCTC TGGGGCAGAA CCCCACAGAA GCAGAGCTGC AGGACATGAT CAATGAAGTA   721 GATGCCGACG GTAATGGCAC AATCGACTTC CCTGAGTTCC TGACAATGAT GGCAAGAAAA   781 ATGAAAGACA CAGACAGTGA AGAAGAAATT AGAGAAGCGT TCCGTGTGTT TGANANNGAT   841 GGCAATGGCT ACNTCAGTGC ANCAGANCTT CNCCACGTGA TGACAANNCT TGGANAGAAN   901 TTAACANATG AANANGTTGA NGAAANTGAT CAGGGANNCA NNNNTNNNTG GGGNNNGNNC   961 AGGNNAANNA CNNNNNTTNN NANNAANNAT GNNNNCNAAN NGAANNCGTN NTNNNNNANN 1021 NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl