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pMXs-3xFLAG-KLF4(L507N) (#RDB19025)

Retroviral expression vector of human KLF4, mutant L507N.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (L507N) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19025 pMXs-3xFLAG-KLF4(L507N) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(L507N) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19025).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19025_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(L507N) 5')
Sequence file: RDB19025_B1Eca.seq check
>D07716C4_B1Ec_2_MMLV-pol_F_E02_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNATCGNN GCTTGGNNAC NCGCCGCCCN CGTGAAGGCT
   61 GCCGACCCCG GGGGTGGACC ATCCTCTAGA CTGCCGGATC TAGCTAGTTA ATTAAGGATC
  121 CCAGTGTGGT GGTACGGGAA TTCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC
  181 ATGACATCGA CTACAAGGAT GACGATGACA AGGCTGTCAG CGACGCGCTG CTCCCATCTT
  241 TCTCCACGTT CGCGTCTGGC CCGGCGGGAA GGGAGAAGAC ACTGCGTCAA GCAGGTGCCC
  301 CGAATAACCG CTGGCGGGAG GAGCTCTCCC ACATGAAGCG ACTTCCCCCA GTGCTTCCCG
  361 GCCGCCCCTA TGACCTGGCG GCGGCGACCG TGGCCACAGA CCTGGAGAGC GGCGGAGCCG
  421 GTGCGGCTTG CGGCGGTAGC AACCTGGCGC CCCTACCTCG GAGAGAGACC GAGGAGTTCA
  481 ACGATCTCCT GGACCTGGAC TTTATTCTCT CCAATTCGCT GACCCATCCT CCGGAGTCAG
  541 TGGCCGCCAC CGTGTCCTCG TCAGCGTCAG CCTCCTCTTC GTCGTCGCCG TCGAGCAGCG
  601 GCCCTGCCAG CGCGCCCTCC ACCTGCAGCT TCACCTATCC GATCCGGGCC GGGAACGACC
  661 CGGGCGTGGC GCCGGGCGGC ACGGGCGGAG GCCTCCTCTA TGGCAGGGAG TCCGCTCCCC
  721 CTCCGACGGC TCCCTTCNAC CTGGCGGACN TCAACGACGT GAGCCCCTCG GGCGGCTTCG
  781 TGGCCGANCT CCTGCGGCCA GAATTGGACC CGGTGTACAT TCCGCCGCAN CAGCCGCAGC
  841 CGCCAGGNGG CGGGCTGATG GNNAAGTTCG TGCTGAAGGC GTCNCTGANC GCCCCTGGCA
  901 NCNAGTACNG NAGCCCGNNN NTCNTCANCG TCNGCAAAGG CAGCCNNNNN NGCAGCCACC
  961 CGGNGGNGGT GGCGCCCTAC NNCGGCGGGN CNNCGCNNAC NNNCCNNNNA TNNAGCNNNA
1021 NGNGNNNNCN TNNNNNNNCC NNNNGGGNNN NNNNNNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert 3'(attB2,hKLF4(L507N) 3')
Sequence file: RDB19025_B1Ecb.seq check
>D07716C4_B1Ec_2_pMXs-3LTRup_R2_F09_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTN ANCGTCGANT NNNNNNGTAC ATAATGGATT TCCTTACGCG
   61 AAATACGGGC ANNCATGGCC TGCCCGGTTA TTATTATTTT TGACACCAGA CCAACTGGTA
  121 ATGGTAGCGA CCGGCGCTCA GCTGGAATAT CACCACTTTG TACAAGAAAG CTGGGTCGGC
  181 GCGCCCACCC TTTTAAAAAT GTCTCTTCAT GTGGTTGGCG AGGTGGTCCG ACCTGGAAAA
  241 TGCTCGGTCG CATTTTTGGC ACTGGAACGG GCGGTGCCCC GTGTGTTTAC GGTAGTGCCT
  301 GGTCAGTTCA TCTGAGCGGG CGAATTTCCA TCCACAGCCG TCCCAGTCAC AGTGGTAAGG
  361 TTTCTCACCT GTGTGGGTTC GCAGGTGTGC CTTGAGATGG GAACTCTTTG TGTAGGTTTT
  421 GCCGCAGCCC GCGTAATCAC AAGTGTGGGT GGCGGTCCTT TTCCGGGGCC ACGATCGTCT
  481 TCCCCTCTTT GGCTTGGGCT CCTCTGGCAT GCAGGAACCG GGTGGCATGA GCTCTTGGTA
  541 ATGGAGCGGC GGGACTTGCG GCTGCATCTG ATCGGGCAGG AAGGATGGGT AATTGGGCCC
  601 CGGGTGGGGA TGGAAGCCGG GAGGAAGCGG CAGGGCAGGG TGACAGTCCC TGCTGCTCAG
  661 CACTTCCTCA AGACCCAGGG TCGGGGTAGT CCTGCTGGGG AGCTGCCGCC CCAGGGGGAA
  721 GTCGTGTGCA GCCGGCCGGT GGCCATTGCT GANAGGGGGT CCAGCGCCCA AGTGGGTGCA
  781 CGAAGAGACC GCCTCCTGCT TGNTCTTGGG GCACGTGCGC GGCGGCCCGC CGTTGTNNGN
  841 CGCCACCANC ACCGGGNGGC TGCCGTCNNG NCTGCCTTTG CTGACGCTGA TGANCNANGG
  901 GNTGCCNNAN TCGCTGNCAG GGNNNNNNAN NNNCNNNTNC NNNACGAANN NGNCNNNNNN
961 NCNNNNNNNN NN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19025_B1Ecc.seq check
>D07716C4_B1Ec_2_gag-N_R_G09_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNTNNTTTTN NGANAAATAC NGAAACNCAG TCAGACAGAG
   61 ACAACACAGA ACGATGCTGC AGCAGACAAG ACGCGCGGCG CGGCTTCGGT CCCAAACCGA
  121 AAGCAAAAAT TCAGACGGAG GCGGGAACTG TTTTAGGTTC TCGTCTCCTA CCAGAACCAC
  181 ATATCCCTCC TATTAGGGGG GTGCACCAAA GAGTCCAAAA CGATCGGGAT TTTTGGACTC
  241 AGGTCGGGCC ACAAAAACGG CCCCCGAAGT CCCTGGGACG TCTCCCAGGG TTGCGGCCGG
  301 GTGTTCCGAA CTCGTCAGTT CCACCACGGG TCCGCCAGAT ACAGAGCTAG TTAGCTAACT
  361 AGTACCGACG CAGGCGCATA AAATCAGTCA TAGACACTAG ACAATCGGAC AGACACAGAT
  421 AAGTTGCTGG CCAGCTTACC TCCCGGTGGT GGGTCGGTGG TCCCTGGGCA GGGGTCTCCC
  481 GATCCCGGAC GAGCCCCCAA ATGAAAGACC CCCGCTGACG GGTAGTCAAT CACTCAGAGG
  541 AGACCCTCCC AAGGAACAGC GAGACCACAA GTCGGATGCA ACTGCAAGAG GGTTTATTGG
  601 ATACACGGGT ACCCGGGCGA CTCAGTCAAT CGGAGGACTG GCGCGCCGAG TGAGGGGTTG
  661 TGGGCTCTTT TATTGAGCTC GGGGANCAGA AGCGCGCGAA CAGAAGCGAG AAGCGAACTG
  721 ATTGGTTAGT TCAAATAANG CACANGGTCA TTTCANGTCC TTNNGGCACC CTGNAAACAT
  781 CTGATGGTTC TCTAGAAACT GCTGNNGGCT GGNNCGCATC TGGGGNNCAT CTGTNCTTGG
  841 CCNNNANCCG GGGCAGGANN NGCTNANCAC ANATATCCNG TTNNGNCNAT ANTCAGCTNN
901 NCCATNNGNT CNNNNNNNNN N
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19025_B1Ecd.seq check
>D07716C4_B1Ec_2_lacIpro-B_F_H09_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NCNNNTCNGT GNNCGNNGAA NCGGAANNNC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA
  121 AATACTTAAG AAAAAAAACC AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC
  181 TGACGGGTAG TCAATCACTC AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG
  241 ATGCAACTGC AAGAGGGTTT ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG
  301 GACTGGCGCC CCGAGTGAGG GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC
  361 GCGAACAGAA GCGAGAAGCG AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA
  421 GGTCCTTGGG GCACCCTGGA AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC
  481 GCATCTGGGG ACCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA
  541 TCCTGTTTGG CCCATATTCA GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC
  601 TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG
  661 ATCTGAACTT CTCTATTCTC AGTTATGTAT TTTTCCATGC CTTGCAAAAT GGCGTTACTT
  721 AAGCTAGCTT GCCAAACCTA CAGGTGGGGT CTTTCATTCC CCCCTTTTTC TGGAGACTAA
  781 ATAAAATCTT TTATTTTATC GTCGACTTTA AATAGTACAT AATGGANTTC CTTACGCGAA
  841 ATACNGGCAG ACATGGNCTG NCCNGNNATT ATTANTTTTG ACACNANNNC NNCNGGNAAT
  901 GGNANCGANN GGNGCTCANC NNGNNNTATC ANCNNCNTNN NNNNNNAANC NNGNNNNNNN
961 NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]