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pMXs-3xFLAG-KLF4(S500A) (#RDB19019)

Retroviral expression vector of human KLF4, mutant S500A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (S500A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19019 pMXs-3xFLAG-KLF4(S500A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(S500A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19019).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19019_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(S500A) 5')
Sequence file: RDB19019_B1Eca.seq check
>D07716B7_B1Ec_1_MMLV-pol_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG NNTCGCAGCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC
   61 GACCCCGGGG GTGGANCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA
  121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG
  181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT
  241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA
  301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC
  361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG
  421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG
  481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG
  541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC
  601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG
  661 GCGTGGCGCC GGGCGGCACG GGCGGANGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC
  721 CGACGGCTCC CTTCAACCTG GCGGANATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG
  781 CCGANCTCCT GCGGCCAGAA TTGGACCCGG TGTACATTCC GCCGCAGCAG CCGCAGCCGC
  841 CAGGTGGCGG GCTGATGGGC AAGTTCGTGC TGAANGCGTC GCTGANCGNC CCTGGCAGCG
  901 AGNACGGCAG CCCGNNNNNC ATCAGCGNCA GCAAANGCAG CCCNGANNGN AGNCACCCNG
  961 NNGGTGGTGG CGCCCTANAN NNGCNGNNNG NCGCNCANNN NCCCNANNTN NANNNNNANG
1021 NNGNTNTNNN NNNGNNNNNN NNNN
//
Primer: hKLF4-mut check_R2 (Pr1009)
Region: insert 3'(hKLF4(S500A) 3',3' LTR
Sequence file: RDB19019_B1Ecb.seq check
>D07716B7_B1Ec_1_hKLF4-mut check_R2_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNNN NNNNNNCCCG GGGCCCNATT ACCCATCCTT
   61 CCTGCCCGAT CAGATGCAGC CGCAAGTCCC GCCGCTCCAT TACCAAGAGC TCATGCCACC
  121 CGGTTCCTGC ATGCCAGAGG AGCCCAAGCC AAAGAGGGGA AGACGATCGT GGCCCCGGAA
  181 AAGGACCGCC ACCCACACTT GTGATTACGC GGGCTGCGGC AAAACCTACA CAAAGAGTTC
  241 CCATCTCAAG GCACACCTGC GAACCCACAC AGGTGAGAAA CCTTACCACT GTGACTGGGA
  301 CGGCTGTGGA TGGAAATTCG CCCGCTCAGA TGAACTGACC AGGCACTACC GTAAACACAC
  361 GGGGCACCGC CCGTTCCAGT GCCAAAAATG CGACCGAGCA TTTGCCAGGT CGGACCACCT
  421 CGCCTTACAC ATGAAGAGGC ATTTTTAAGC GGCCGCCAGC ACAGTGGTCG ACGATAAAAT
  481 AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG
  541 GCAAGCTAGC TTAAGTAACG CCATTTTGCA AGGCATGGAA AAATACATAA CTGAGAATAG
  601 AGAAGTTCAG ATCAAGGTCA GGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT
  661 CTGTGGTAAG CAGTTCCTGC CCCGGCTCAN GGCCAAGAAC AGATGGAACA GCTGAATATG
  721 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCANGGCCN AGAACAGATG
  781 GTCCCCAGAT GCGGTCCAGC CCTCAGCAGT TTCTAGAGAA NCATCAGANG TTTCCAGGGT
  841 GCCCCAAGGA CCTGAAATGA NCCTGTGCCT TANTTGAACT AACCAATCAN TTCGCTNNNC
  901 GCTNNNGTNC NCGNGCTTCT GCTCCNNNAN CNCNATAAAA NANCCNNNAA NNNNNNNNNC
961 GGGNNNNCNN NNNNNNNANN GNNNNANNNN NNNNGNNNNN NNNNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert 3'(hKLF4(S500A) 3'
Sequence file: RDB19019_B1Ecc.seq check
>D07716B7_B1Ec_1_pMXs-3LTRup_R2_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTTNNTCG TCGANNNCTG TGCTGNNCGG CCGCTTAAAA
   61 ATGCCTCTTC ATGTGTAAGG CGAGGTGGTC CGACCTGGCA AATGCTCGGT CGCATTTTTG
  121 GCACTGGAAC GGGCGGTGCC CCGTGTGTTT ACGGTAGTGC CTGGTCAGTT CATCTGAGCG
  181 GGCGAATTTC CATCCACAGC CGTCCCAGTC ACAGTGGTAA GGTTTCTCAC CTGTGTGGGT
  241 TCGCAGGTGT GCCTTGAGAT GGGAACTCTT TGTGTAGGTT TTGCCGCAGC CCGCGTAATC
  301 ACAAGTGTGG GTGGCGGTCC TTTTCCGGGG CCACGATCGT CTTCCCCTCT TTGGCTTGGG
  361 CTCCTCTGGC ATGCAGGAAC CGGGTGGCAT GAGCTCTTGG TAATGGAGCG GCGGGACTTG
  421 CGGCTGCATC TGATCGGGCA GGAAGGATGG GTAATTGGGC CCCGGGTGGG GATGGAAGCC
  481 GGGAGGAAGC GGCAGGGCAG GGTGACAGTC CCTGCTGCTC AGCACTTCCT CAAGACCCAG
  541 GGTCGGGGTA GTCCTGCTGG GGAGCTGCCG CCCCAGGGGG AAGTCGTGTG CAGCCGGCCG
  601 GTGGCCATTG CTGAGAGGGG GTCCAGCGCC CAAGTGGGTG CACGAAGAGA CCGCCTCCTG
  661 CTTGATCTTG GGGCACGTGC GCGGCGGCCC GCCGTTGTAG GGCGCCACCA CCACCGGGTG
  721 GCTGCCGTCA GGGCTGCCTT TGCTGACGCT GATGACCGAC GGGCTGCCGT ACTCGCTGCC
  781 AGGGGCGCTC NNCGACGCCT TCNGCACGAA CTTGCCCATC AGCCCGCCNC CTGGCGGCTG
  841 CGGCTGCTGC GGCGGANTGT ACACCGGGGN CCNATTCTGG NNCGCANGAN CTCGGCNNCN
  901 ANNNCNNCCG ANGGGCTCAC GTCGTTGATG TCCGCCAGGN TGAANGGAGC CGTCGGANGG
  961 GGANCGNANN NCCTGCNNTA NANGANNNNT CCGCCCGTGN NNCNNGCGCC ACGCCCNGGN
 1021 NNNNNCCNGN CCNNATNNGN NNNNGTGNAN NNNCNNNNNN NNGGNNNNCN NGNNNNGNNN
1081 NNNNNNN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19019_B1Ecd.seq check
>D07716B7_B1Ec_1_gag-N_R_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NTTNNNNNAC NAAATACAGA AACACAGTCN
   61 GACAGAGACA ACACAGAACG ATGCTGCAGC AGACAAGACG CGCGGCGCGG CTTCGGTCCC
  121 AAACCGAAAG CAAAAATTCA GACGGAGGCG GGAACTGTTT TAGGTTCTCG TCTCCTACCA
  181 GAACCACATA TCCCTCCTAT TAGGGGGGTG CACCAAAGAG TCCAAAACGA TCGGGATTTT
  241 TGGACTCAGG TCGGGCCACA AAAACGGCCC CCGAAGTCCC TGGGACGTCT CCCAGGGTTG
  301 CGGCCGGGTG TTCCGAACTC GTCAGTTCCA CCACGGGTCC GCCAGATACA GAGCTAGTTA
  361 GCTAACTAGT ACCGACGCAG GCGCATAAAA TCAGTCATAG ACACTAGACA ATCGGACAGA
  421 CACAGATAAG TTGCTGGCCA GCTTACCTCC CGGTGGTGGG TCGGTGGTCC CTGGGCAGGG
  481 GTCTCCCGAT CCCGGACGAG CCCCCAAATG AAAGACCCCC GCTGACGGGT AGTCAATCAC
  541 TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC GGATGCAACT GCAAGAGGGT
  601 TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG AGGACTGGCG CGCCGAGTGA
  661 GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC GCGCGAACAG AAGCGAGAAG
  721 CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT CAGGTCCTTG GGGCANCCTG
  781 GAAACATCTG ATGGTTCTCT ANAAACTGCT GANGGCTGGA CCGCATCTGG GGANCATCTG
  841 TTCTTGGNCC TGANCCGGGG CANGAACTGC TTACCACAGA TATCCNGTTN GGNCCANNTT
  901 CNGCTGTTCC ATCTGTTCTT GGCCCTGANC CGGGGCANGA NNNGCTNACC NCAGATATCC
  961 TGNTNNNNCC ATATTCAGCT GTTCNNTCTG TNCCNGANCN NGNNCTGNAN TTTTCNATTC
1021 TCNNNNATGN NNTTTNNNNN NNCNNNNNNA ANNNGNNNNN NNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19019_B1Ece.seq check
>D07716B7_B1Ec_1_lacIpro-B_F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCGNN NNNNTGAGCG AGGNNNCGGA AGAGCGCCCA
   61 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAACTA TGGCCATTTA
  121 ATGTAAATAC TTAAGAAAAA AAACCAAATT AATTTTGATA CATGCTGCAT GTGAAAGACC
  181 CCCGCTGACG GGTAGTCAAT CACTCAGAGG AGACCCTCCC AAGGAACAGC GAGACCACAA
  241 GTCGGATGCA ACTGCAAGAG GGTTTATTGG ATACACGGGT ACCCGGGCGA CTCAGTCAAT
  301 CGGAGGACTG GCGCCCCGAG TGAGGGGTTG TGGGCTCTTT TATTGAGCTC GGGGAGCAGA
  361 AGCGCGCGAA CAGAAGCGAG AAGCGAACTG ATTGGTTAGT TCAAATAAGG CACAGGGTCA
  421 TTTCAGGTCC TTGGGGCACC CTGGAAACAT CTGATGGTTC TCTAGAAACT GCTGAGGGCT
  481 GGACCGCATC TGGGGACCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC TGCTTACCAC
  541 AGATATCCTG TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CTTGGCCCTG AGCCGGGGCA
  601 GGAACTGCTT ACCACAGATA TCCTGTTTGG CCCATATTCA GCTGTTCCAT CTGTTCCTGA
  661 CCTTGATCTG AACTTCTCTA TTCTCAGTTA TGTATTTTTC CATGCCTTGC AAAATGGCGT
  721 TACTTAAGCT AGCTTGCCAA ACCTACAGGT GGGGTCTTTC ATTCCCCCCT TTTTCTGGAN
  781 ACTAAATAAA ATCTTTTATT TTATCGTCGA NCACTGTGCT GGCGGCCGCT TAAAAATGCC
  841 TCTTCATGTG TAANNNGANG TGGTCCGANC TGGCAAATGC TCGGTCGCAT TTTTGNNNCT
  901 GGANNGGGCG GNGNCCCGTG TGTTTACGGN ANNGCCNNNN NNNNTCNTCT GANCGGGCNA
  961 NNTTNNNTCC ACAGNNNNNC CNNTCACNNN GGNNNNNTTT CNCNNCTGTG TGGGNTNNCN
1021 NNNTGNCNTN GANANNNNNN NNCNTTNNNN NNNNTTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]