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pMXs-3xFLAG-KLF4(R473A) (#RDB19012)

Retroviral expression vector of human KLF4, mutant R473A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (R473A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19012 pMXs-3xFLAG-KLF4(R473A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(R473A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19012).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19012_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(R473A) 5')
Sequence file: RDB19012_B1Eca.seq check
>D07716A9_B1Ec_1_MMLV-pol_F_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG NATCGNAGCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC
   61 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA
  121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG
  181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT
  241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA
  301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC
  361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG
  421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG
  481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG
  541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC
  601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG
  661 GCGTGGCGCC GGGCGGCACG GGCGGAGGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC
  721 CGACGGCTCC CTTCAACCTG GCGGACATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG
  781 CCGANCTCCT GCGGCCAGAA TTGGACCCGG NGTACATTCC GCCGCANCAG CCGCAGCCGC
  841 CAGGTGNCGG GCTGATGGGC AAGTTCGTGC TGAAGGCGTC GCTGANCGCC CNNNGCAGCN
  901 NNTACNGCAG CCCGNCNGNC ATCAGCGTCA GCAAANGNAG CCNNGANNNN NNCCNNNNNG
961 NGGNNGNNNN NNNNNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert 3'(hKLF4(R473A) 3')
Sequence file: RDB19012_B1Ecb.seq check
>D07716A9_B1Ec_1_pMXs-3LTRup_R2_B04_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTN NTCGTCGNNN NCTGTGCTGG CGGCCGCTTA AAAATGCCTC
   61 TTCATGTGTA AGGCGAGGTG GTCCGACCTG GAAAATGCTC GGTCGCATTT TTGGCACTGG
  121 AACGGGCGGT GCCCCGTGTG TTTACGGTAG TGCCTGGTCA GTTCATCTGA GGCGGCGAAT
  181 TTCCATCCAC AGCCGTCCCA GTCACAGTGG TAAGGTTTCT CACCTGTGTG GGTTCGCAGG
  241 TGTGCCTTGA GATGGGAACT CTTTGTGTAG GTTTTGCCGC AGCCCGCGTA ATCACAAGTG
  301 TGGGTGGCGG TCCTTTTCCG GGGCCACGAT CGTCTTCCCC TCTTTGGCTT GGGCTCCTCT
  361 GGCATGCAGG AACCGGGTGG CATGAGCTCT TGGTAATGGA GCGGCGGGAC TTGCGGCTGC
  421 ATCTGATCGG GCAGGAAGGA TGGGTAATTG GGCCCCGGGT GGGGATGGAA GCCGGGAGGA
  481 AGCGGCAGGG CAGGGTGACA GTCCCTGCTG CTCAGCACTT CCTCAAGACC CAGGGTCGGG
  541 GTAGTCCTGC TGGGGAGCTG CCGCCCCAGG GGGAAGTCGT GTGCAGCCGG CCGGTGGCCA
  601 TTGCTGAGAG GGGGTCCAGC GCCCAAGTGG GTGCACGAAG AGACCGCCTC CTGCTTGATC
  661 TTGGGGCACG TGCGCGGCGG CCCGCCGTTG TAGGGCGCCA CCACCACCGG GTGGCTGCCG
  721 TCAGGGCTGC CTTTGCTGAC GCTGATGANC GACGGGCTGC CGTACTCGCT GCCANGGGCG
  781 CTCAGCGACG CCTTCANCAC GAACTTGCCC ATCAGCCCGC CACCTGGCGG CTGCGGCTGC
  841 TGCGNCGGAN NGTACACCGG GTCCNATNCT GGNCGCANGA NCTCGGCCNC NANNNNGCCC
  901 NANGGGCTCN CGTCNTNNAT GTNCNCNNGG TTNAANGNNN NNGTCGGANG GGGNNCGGAC
961 TNCCTNNNNN ANNNGANNNC NNNNNNNNNG NNNNNNNNNN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19012_B1Ecc.seq check
>D07716A9_B1Ec_1_gag-N_R_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTTTTNNGAC AAATACAGAA ACACAGTCAG
   61 ACAGAGACAA CACAGAACGA TGCTGCAGCA GACAAGACGC GCGGCGCGGC TTCGGTCCCA
  121 AACCGAAAGC AAAAATTCAG ACGGAGGCGG GAACTGTTTT AGGTTCTCGT CTCCTACCAG
  181 AACCACATAT CCCTCCTATT AGGGGGGTGC ACCAAAGAGT CCAAAACGAT CGGGATTTTT
  241 GGACTCAGGT CGGGCCACAA AAACGGCCCC CGAAGTCCCT GGGACGTCTC CCAGGGTTGC
  301 GGCCGGGTGT TCCGAACTCG TCAGTTCCAC CACGGGTCCG CCAGATACAG AGCTAGTTAG
  361 CTAACTAGTA CCGACGCAGG CGCATAAAAT CAGTCATAGA CACTAGACAA TCGGACAGAC
  421 ACAGATAAGT TGCTGGCCAG CTTACCTCCC GGTGGTGGGT CGGTGGTCCC TGGGCAGGGG
  481 TCTCCCGATC CCGGACGAGC CCCCAAATGA AAGACCCCCG CTGACGGGTA GTCAATCACT
  541 CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT
  601 TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC GCCGAGTGAG
  661 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG ANCAGAAGCG CGCGAACAGA AGCGAGAAGC
  721 GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC ANGTCCTTGG GGCACCCTGG
  781 AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAN CGCATCTGGG GANCATCTGT
  841 TCTTGGNCCT GANCCNGGGC ANGAACTGCT TACCACAGAT ATCCTGTTNN GCCCNTATTC
  901 AGCNNNTNCA TCNGNTCTNN GNCCNNANNC NGGGGNNNNN NNNGNNNNNN NNANNNATNN
961 NGTTTNGNCN NNANNNNGCN GNTNNNNNNN NNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19012_B1Ecd.seq check
>D07716A9_B1Ec_1_lacIpro-B_F_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNNNNNNTNN GTGANCGANG AANCGGAAGA NCGCCCAATA
   61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAACTATGG CCATTTAATG
  121 TAAATACTTA AGAAAAAAAA CCAAATTAAT TTTGATACAT GCTGCATGTG AAAGACCCCC
  181 GCTGACGGGT AGTCAATCAC TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC
  241 GGATGCAACT GCAAGAGGGT TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG
  301 AGGACTGGCG CCCCGAGTGA GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC
  361 GCGCGAACAG AAGCGAGAAG CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT
  421 CAGGTCCTTG GGGCACCCTG GAAACATCTG ATGGTTCTCT AGAAACTGCT GAGGGCTGGA
  481 CCGCATCTGG GGACCATCTG TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA
  541 TATCCTGTTT GGCCCATATT CAGCTGTTCC ATCTGTTCTT GGCCCTGAGC CGGGGCAGGA
  601 ACTGCTTACC ACAGATATCC TGTTTGGCCC ATATTCAGCT GTTCCATCTG TTCCTGACCT
  661 TGATCTGAAC TTCTCTATTC TCAGTTATGT ATTTTTCCAT GCCTTGCAAA ATGGCGTTAC
  721 TTAAGCTAGC TTGCCNAACC TACAGGTGGG GTCTTTCATT CCCCCCTTTT TCTGGAGACT
  781 NAANTAAAAT CTTTTATTTT ATCGTCNANC ACTGTGCTGG CGGCCGCTTA AAANTGCCTC
  841 TTCATGTGTA ANGNGAGGTG GNCCGACCTG GAAAATGNTC GGTCGCNTTT TTGGCACNNG
  901 AACGGGNNGN GCCCCGNGTG TTTACNGNAG TGNCTGGNCA NTTCATCTGN NNNGCGAANT
961 TTCCNTCCNC AGCCNNCCNN NNCNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]