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pMRX-puro-FLAG-hVPS52_N670

Retroviral expression vector of human VPS52 C-terminal deletion mutant tagged with FLAG at N-terminus.

Catalog number RDB18999
Resource name pMRX-puro-FLAG-hVPS52_N670
Clone info. Retroviral expression vector of human VPS52 subunit of GARP complex (VPS52) mutant (C-terminal deletion mutant, 671-723aa was deleted) tagged with FLAG at N-terminus, see Fig3A of Homma, Y. & Fukuda, M. Biochem. Biophys. Res. Commun. 561: 151-157 (2021).
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-puro (plasmid)
Gene/insert name human VPS52 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
human VPS52
          Reference sequence
            
           
          Sequence (full) RDB18999zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Homma, Y. & Fukuda, M. Biochem. Biophys. Res. Commun. 561: 151-157 (2021)).
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Homma, Y. & Fukuda, M. Biochem. Biophys. Res. Commun. 561: 151-157 (2021))。
          付加的提供条件：
          pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18999 pMRX-puro-FLAG-hVPS52_N670 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Homma, Y., Knockout analysis of Rab6 effector proteins revealed the role of VPS52 in the secretory pathway. Biochem. Biophys. Res. Commun. 561: 151-157 (2021). PMID 34023780.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Homma, Y., Knockout analysis of Rab6 effector proteins revealed the role of VPS52 in the secretory pathway. Biochem. Biophys. Res. Commun. 561: 151-157 (2021). PMID 34023780.

          2022.10.11

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