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pEF-human MFG-E8/Flag (#RDB18995)

Mammalian expression vector of human MFGE8 (MFG-E8) tagged with FLAG at C-terminus.

Clone info. Mammalian expression vector of human milk fat globule EGF and factor V/VIII domain containing (MFGE8, Aliases: MFG-E8) tagged with FLAG at C-terminus. See Fig.1A of Yamaguchi, H. et al. J. Leukoc. Biol. 83 (5): 1300-1307, 2008.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pEF-BOS-EX (plasmid)
Size of vector backbone 5.3 kb
Selectable markers Ampicillin
Gene/insert name human MFGE8 cDNA
Depositor|Developer Nagata, Shigekazu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Yamaguchi, H. et al., J. Leukoc. Biol., 83 (5): 1300-1307, 2008). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yamaguchi, H. et al., J. Leukoc. Biol., 83 (5): 1300-1307, 2008)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18995 pEF-human MFG-E8/Flag Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pEF-human MFG-E8/Flag was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18995).

Reference section:

Yamaguchi, H., Takagi, J., Miyamae, T., Yokota, S., Fujimoto, T., Nakamura, S., Ohshima, S., Naka, T., Nagata, S., Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus. J. Leukoc. Biol. 2008 May;83(5):1300-1307 (2008). PMID 18303131. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Yamaguchi, H., Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus. J. Leukoc. Biol. 2008 May;83(5):1300-1307 (2008). PMID 18303131. [link to RRC of NBRP]