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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Tim1-Fc

Mammalian expression vector of the extracellular region of mouse Havcr1 (Tim1) (aa 1-199).

Catalog number RDB18986
Resource name pEF-Tim1-Fc
Clone info. Mammalian expression vector of the extracellular region of mouse hepatitis A virus cellular receptor 1 (Havcr1, Aliases: Tim1) (aa 1-199) with a splice donor (GTAAGTC). See Fig.3f of Miyanishi, M. et al. Nature, 450 (7168): 435-439, 2007.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pEF-Fc (plasmid)
Selectable markers Ampicillin
Gene/insert name mouse Havcr1 cDNA
Depositor|Developer Nagata, Shigekazu |
Other clones in our bank

External Database
mouse Havcr1

          Reference sequence
            
           
          Sequence (full) RDB18986zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
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          手続きの概要は、「提供申込みについて[link]」をご覧ください。

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          RDB18986 pEF-Tim1-Fc DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEF-Tim1-Fc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18986).

          Reference section:

          Miyanishi, M., Tada, K., Koike, M., Uchiyama, Y., Kitamura, T., Nagata, S., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18986_B1Drp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F (Pr0168)
          Region: insert 5'(mTim1(aa 1-199) 5')
          Sequence file: RDB18986_B1Dra.seq check
          >D07990C5_B1Dr_2_EF1a-C_F_A04_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNANTTT GGANCTTGGT TNNTTCTCNA GCCTCAGACA
             61 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTCTCTA GAGATCCCTC
            121 GACCTCNAGA TCCATCGGGC CCCCCCTCGA GGTCGACGTC AGTACCATGA ATCAGATTCA
            181 AGTCTTCATT TCAGGCCTCA TACTGCTTCT CCCAGGCGCT GTGGATTCTT ATGTGGAAGT
            241 AAAGGGGGTG GTGGGTCACC CTGTCACACT TCCATGTACT TACTCAACAT ATCGTGGAAT
            301 CACAACGACA TGTTGGGGCC GAGGGCAATG CCCATCTTCT GCTTGTCAAA ATACACTTAT
            361 TTGGACCAAT GGACATCGTG TCACCTATCA GAAGAGCAGT CGGTACAACT TAAAGGGGCA
            421 TATTTCAGAA GGAGATGTGT CCTTGACGAT AGAGAACTCT GTTGAGAGTG ACAGTGGTCT
            481 GTATTGTTGT CGAGTGGAGA TTCCTGGATG GTTTAATGAT CAGAAAGTGA CCTTTTCATT
            541 GCAAGTTAAA CCAGAGATTC CCACACGTCC TCCAAGAAGA CCCACAACTA CAAGGCCCAC
            601 AGCTACAGGA AGACCCACGA CTATTTCAAC AAGATCCACA CATGTACCAA CATCAACCAG
            661 AGTCTCTACC TCCACTCCTC CAACATCTAC ACACACATGG ACTCACAAAC CAGACTGGAA
            721 TGGCACTGTG ACATCCTCAN GAGATACCTG GAGTAATCAC ACTAGTAAGT CGATATCGAA
            781 TTCCTGCAGC CCGGGGGANC CACTAGTTCT ATCCTGCCTG GACGCATCCC GGCTATGCAG
            841 TCCCAGTCCA GGGCANCAAG GCANGNCCCG NCTGCCTCTT CNCCCGGAGG NNTCTGNCCG
            901 CCCCACTCAT GCTCNNGGAN ANGGNCTNCT GGNTTTTTNC CCNNNCTCTG GNNNNNCACN
          961 NGCNNNNGCC CCNNNNCNNN NCCNGNNNAN NNNGGGCNNN GCTGGGCTCN NNNNNNNNN
          //
          Primer: hIgG-genome_R1 (Pr1105)
          Region: IgG Fc-region,insert 3'(splice donor site,mTim1(aa1-199) 3')
          Sequence file: RDB18986_B1Drb.seq check
          >D07990C5_B1Dr_2_hlgG-genome_R1_C04_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGTC TGANCCCNGC ACCTGCCCCT TTGTGTGCAG
             61 GGCCTGGGTT AGGGGCACCT AGCCTGTGCC TGCCCAGAGC CTGGGGAAAA AGCCAGAAGA
            121 CCCTCTCCCT GAGCATGAGT GGGGCGGGCA GAGGCCTCCG GGTGAAGAGG CAGACGGGGC
            181 CTGCCTTGCT GCCCTGGACT GGGACTGCAT AGCCGGGATG CGTCCAGGCA GGATAGAACT
            241 AGTGGATCCC CCGGGCTGCA GGAATTCGAT ATCGACTTAC TAGTGTGATT ACTCCAGGTA
            301 TCTCCTGAGG ATGTCACAGT GCCATTCCAG TCTGGTTTGT GAGTCCATGT GTGTGTAGAT
            361 GTTGGAGGAG TGGAGGTAGA GACTCTGGTT GATGTTGGTA CATGTGTGGA TCTTGTTGAA
            421 ATAGTCGTGG GTCTTCCTGT AGCTGTGGGC CTTGTAGTTG TGGGTCTTCT TGGAGGACGT
            481 GTGGGAATCT CTGGTTTAAC TTGCAATGAA AAGGTCACTT TCTGATCATT AAACCATCCA
            541 GGAATCTCCA CTCGACAACA ATACAGACCA CTGTCACTCT CAACAGAGTT CTCTATCGTC
            601 AAGGACACAT CTCCTTCTGA AATATGCCCC TTTAAGTTGT ACCGACTGCT CTTCTGATAG
            661 GTGACACGAT GTCCATTGGT CCAAATAAGT GTATTTTGAC AAGCAGAAGA TGGGCATTGC
            721 CCTCGGCCCC AACATGTCGT TGTGATTCCA CGATATGTTG AGTAAGTACA TGGAAGTGTG
            781 ACAGGGTGAC CCACCACCCC CTTTACTTCC NCATAAGAAT CCACAGCGCC TGGGANAAGC
            841 AGTATGAGGC CTGAAATGAA GACTTGAATC TGATTCATGG TACTGACGTC GACCTCNNNG
            901 GGGGGNCCGA TGGATCTCGA GGTCNANGGA TCTCTAGAGA ATTNCTCACG ANNCCTGAAN
            961 TGGAAGAAAA AAANTTTGAN NCNCTGNNTG ANGNTTGANN ATGNANNNNN NATCNNAACT
          1021 CNAAANNGNN NNNTNNNNNN ANANTTNCNN NNNNNNNN
          //
          Primer: hGCSF_R (Pr0031)
          Region: hG-CSF 3'UTR+pA,IgG-Fc-region
          Sequence file: RDB18986_B1Drc.seq check
          >D07990C5_B1Dr_2_hGCSF_R_B04_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNCCCNG GTGGGGNNCC TCACTCTAGA GTCGCGGCCG
             61 CTCTAGGGCA GTGGTGGGTG CTTTATTTCC ATGCTGGGTG CCTGGGAAGT ATGTAGACGG
            121 GGTACGTGCC AAGCATCCTC GTGCGACCGC GAGAGCCCGG GGAGCGGGGG CTTGCCGGCC
            181 GTCGCACTCA TTTACCCGGG GACAGGGAGA GGCTCTTCTG CGTGTAGTGG TTGTGCAGAG
            241 CCTCATGCAT CACGGAGCAT GAGAAGACGT TCCCCTGCTG CCACCTGCTC TTGTCCACGG
            301 TGAGCTTGCT ATAGAGGAAG AAGGAGCCGT CGGAGTCCAG CACGGGAGGC GTGGTCTTGT
            361 AGTTGTTCTC CGGCTGCCCA TTGCTCTCCC ACTCCACGGC GATGTCGCTG GGATAGAAGC
            421 CTTTGACCAG GCAGGTCAGG CTGACCTGGT TCTTGGTCAT CTCCTCCCGG GATGGGGGCA
            481 GGGTGTACAC CTGTGGTTCT CGGGGCTGCC CTGTAGGGAC AGAGGTTGGT ACAGCGGTCA
            541 CTCTCAGGGC AGAGGGTGGG CCGAGCCGGC CTCTGTCCAT GTGGCCCTCG CACCCCACGG
            601 GTCCCACCTT TGGCTTTGGA GATGGTTTTC TCGATGGGGG CTGGGAGGGC TTTGTTGGAG
            661 ACCTTGCACT TGTACTCCTT GCCATTCAGC CAGTCCTGGT GCAGGACGGT GAGGACGCTG
            721 ACCACACGGT ACGTGCTGTT GTACTGCTCC TCCCGCGGCT TTGTCTTGGC NTTATGCACC
            781 TCCACGCCGT CCACGTACCA GTTGAACTTG ACCTCAGGGT CTTCGTGGCT CACGTCCACC
            841 ACCACGCATG TGACCTCANG GGTCNGGGAN ATCATGANGG TGTCCTTGGG TTTTGGGGGG
            901 GAAGAGGAAG ANTGNNGGTC CCCCNNNGAN TNNAGGTGCT GANGAAGAGA TGGAGGTGGA
            961 NGTGTCAGCA CCCNGNNTGG GGCCTGNNCC TGGATGCNNN NNANTCTNGN NNNNNNNNCC
           1021 NNCNNNGANC NNGNNNGNCG ANNNNTNNNN GGNNTNACNN NNGNNNNNNN GNNNNNNNNN
          1081 N
          //
          Primer: Fc-CH2M_R (Pr1251)
          Region: IgG Fc-region
          Sequence file: RDB18986_B1Drd.seq check
          >D07990C5_B1Dr_2_Fc-CH2M_R_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NNNTGNNNNC ACGGTACGTG CTGTTGTNNC
             61 TGCTCCTCCC GCGGCTTTGT CTTGGCATTA TGCACCTCCA CGCCGTCCAC GTACCAGTTG
            121 AACTTGACCT CAGGGTCTTC GTGGCTCACG TCCACCACCA CGCATGTGAC CTCAGGGGTC
            181 CGGGAGATCA TGAGGGTGTC CTTGGGTTTT GGGGGGAAGA GGAAGACTGA CGGTCCCCCC
            241 AGGAGTTCAG GTGCTGAGGA AGAGATGGAG GTGGACGTGT CAGCACCCGG CTGGGGCCTG
            301 TCCCTGGATG CAGGCTACTC TAGGGCACCT GTCCCGCCTT GAGCTGGAGG GCGAGGCCTG
            361 GGCTGGCTTA CCTGGGCACG GTGGGCATGT GTGAGTTTTG TCACAAGATT TGGGCTCTGC
            421 AGAGAGAAGA TTGGGAGTTA CTGGAATCTG GGAGGAGAGA AGGTGTCCGA GCTGAGGGAG
            481 TGGAGAGTTT GGCCTTTGGG GTGGGCTTAG GTCAGGGGCA GGGTCCTCCC GGATATGGCT
            541 CTTGGCAGGT CTGAGCCCAG CACCTGCCCC TTTGTGTGCA GGGCCTGGGT TAGGGGCACC
            601 TAGCCTGTGC CTGCCCAGAG CCTGGGGAAA AAGCCAGAAG ACCCTCTCCC TGAGCATGAG
            661 TGGGGCGGGC AGAGGCCTCC GGGTGAANAN GCAGACGGGG CCTGCCTTGC TGCCCTGGAC
            721 TGGGACTGCA TAGCCGGGAT GCGTCCNGNN NNGATAGAAC TAGTGGATCC CCCGGGCTGN
            781 NNGAATTCGA TATCGACTTA CTAGTGTGAT TACTCCAGGT ATCTCCTGAN GATGTCNCAG
            841 TGCCATTCCA GTCTGGGTTT GTGAGTCCNT GTGTGTGTAG ATGTNNGANG AGTGGANGTA
            901 NANACTCTGG GTTGATGTTN GGTNCATGTG NNGNATCTTG TTGAAATANT NNNTGGGGNC
            961 NTNCNTGTNN NTGTGGGCNN TGNNANTTNN TGGGGTCNTT CNTNGGANGA NNTGTGGGNA
           1021 NTCTCNGGGT TTNNNNNTNN NNANGAAAAN GNTCNCNTTT NNNNATNNNT TAAANCNNNN
          1081 NCNNNGNANT NNNNCNNCNN NNNNNNNN
          //
          Primer: EF1a-N_R (Pr0024)
          Region: SV40 pro_ori,lacO,lac pro
          Sequence file: RDB18986_B1Dre.seq check
          >D07990C5_B1Dr_2_EF1a-N_R_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGCGNNGTG CGCTCTGCCC NCTGACGGGC ACCGGANCCN
             61 ATTCCCACTC CTTTCAAGAC CTAGAAGGTC CATTAGCTGC AAAGATTCCT CTCTGTTTAA
            121 AACTTTATCC ATCTTTGCAA AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC
            181 ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA
            241 TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG
            301 GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC
            361 CTGCTGGGGA GGGAATTAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT
            421 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG
            481 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG
            541 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
            601 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCNNTC GGTCGTTCGG
            661 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
            721 GANAACGCAG GAAAGAACAT GTGAGCAAAA GGNCANCAAA AGGNCAGGAA CCGTAAAAAG
            781 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAANTCGA
            841 CGCTCNNNTC ANAGGTGGCN AAANCCGACA GGANTATAAN NATANNAGGC GTTTCCCCCT
            901 GGAAGCTCCC TCGTGCGNTC NCCNGTTCCN ANCCTGNCNC TTNNCNGGAT ACNNGNNCNC
            961 CTTTCNNCCC NTNNGNAANC GTNNNGCTTT CTNATANNNC NCGCTGNNNN NATNTNNNNN
          1021 NNNGNNGTNN NNNNNTCNNN NNANNNNNGG NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Miyanishi, M., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135. [link to RRC of NBRP]

          2023.10.05

          GNP_filter3_RDBDEP_html_231001.pl