Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-Tim1-Fc

Mammalian expression vector of the extracellular region of mouse Havcr1 (Tim1) (aa 1-199).

Sequence (full)	RDB18986zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

Ordering

Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18986_B1Drp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: insert 5'(mTim1(aa 1-199) 5') Sequence file: RDB18986_B1Dra.seq
>D07990C5_B1Dr_2_EF1a-C_F_A04_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNANTTT GGANCTTGGT TNNTTCTCNA GCCTCAGACA    61 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTCTCTA GAGATCCCTC   121 GACCTCNAGA TCCATCGGGC CCCCCCTCGA GGTCGACGTC AGTACCATGA ATCAGATTCA   181 AGTCTTCATT TCAGGCCTCA TACTGCTTCT CCCAGGCGCT GTGGATTCTT ATGTGGAAGT   241 AAAGGGGGTG GTGGGTCACC CTGTCACACT TCCATGTACT TACTCAACAT ATCGTGGAAT   301 CACAACGACA TGTTGGGGCC GAGGGCAATG CCCATCTTCT GCTTGTCAAA ATACACTTAT   361 TTGGACCAAT GGACATCGTG TCACCTATCA GAAGAGCAGT CGGTACAACT TAAAGGGGCA   421 TATTTCAGAA GGAGATGTGT CCTTGACGAT AGAGAACTCT GTTGAGAGTG ACAGTGGTCT   481 GTATTGTTGT CGAGTGGAGA TTCCTGGATG GTTTAATGAT CAGAAAGTGA CCTTTTCATT   541 GCAAGTTAAA CCAGAGATTC CCACACGTCC TCCAAGAAGA CCCACAACTA CAAGGCCCAC   601 AGCTACAGGA AGACCCACGA CTATTTCAAC AAGATCCACA CATGTACCAA CATCAACCAG   661 AGTCTCTACC TCCACTCCTC CAACATCTAC ACACACATGG ACTCACAAAC CAGACTGGAA   721 TGGCACTGTG ACATCCTCAN GAGATACCTG GAGTAATCAC ACTAGTAAGT CGATATCGAA   781 TTCCTGCAGC CCGGGGGANC CACTAGTTCT ATCCTGCCTG GACGCATCCC GGCTATGCAG   841 TCCCAGTCCA GGGCANCAAG GCANGNCCCG NCTGCCTCTT CNCCCGGAGG NNTCTGNCCG   901 CCCCACTCAT GCTCNNGGAN ANGGNCTNCT GGNTTTTTNC CCNNNCTCTG GNNNNNCACN 961 NGCNNNNGCC CCNNNNCNNN NCCNGNNNAN NNNGGGCNNN GCTGGGCTCN NNNNNNNNN //
Primer: hIgG-genome_R1 (Pr1105) Region: IgG Fc-region,insert 3'(splice donor site,mTim1(aa1-199) 3') Sequence file: RDB18986_B1Drb.seq
>D07990C5_B1Dr_2_hlgG-genome_R1_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGTC TGANCCCNGC ACCTGCCCCT TTGTGTGCAG    61 GGCCTGGGTT AGGGGCACCT AGCCTGTGCC TGCCCAGAGC CTGGGGAAAA AGCCAGAAGA   121 CCCTCTCCCT GAGCATGAGT GGGGCGGGCA GAGGCCTCCG GGTGAAGAGG CAGACGGGGC   181 CTGCCTTGCT GCCCTGGACT GGGACTGCAT AGCCGGGATG CGTCCAGGCA GGATAGAACT   241 AGTGGATCCC CCGGGCTGCA GGAATTCGAT ATCGACTTAC TAGTGTGATT ACTCCAGGTA   301 TCTCCTGAGG ATGTCACAGT GCCATTCCAG TCTGGTTTGT GAGTCCATGT GTGTGTAGAT   361 GTTGGAGGAG TGGAGGTAGA GACTCTGGTT GATGTTGGTA CATGTGTGGA TCTTGTTGAA   421 ATAGTCGTGG GTCTTCCTGT AGCTGTGGGC CTTGTAGTTG TGGGTCTTCT TGGAGGACGT   481 GTGGGAATCT CTGGTTTAAC TTGCAATGAA AAGGTCACTT TCTGATCATT AAACCATCCA   541 GGAATCTCCA CTCGACAACA ATACAGACCA CTGTCACTCT CAACAGAGTT CTCTATCGTC   601 AAGGACACAT CTCCTTCTGA AATATGCCCC TTTAAGTTGT ACCGACTGCT CTTCTGATAG   661 GTGACACGAT GTCCATTGGT CCAAATAAGT GTATTTTGAC AAGCAGAAGA TGGGCATTGC   721 CCTCGGCCCC AACATGTCGT TGTGATTCCA CGATATGTTG AGTAAGTACA TGGAAGTGTG   781 ACAGGGTGAC CCACCACCCC CTTTACTTCC NCATAAGAAT CCACAGCGCC TGGGANAAGC   841 AGTATGAGGC CTGAAATGAA GACTTGAATC TGATTCATGG TACTGACGTC GACCTCNNNG   901 GGGGGNCCGA TGGATCTCGA GGTCNANGGA TCTCTAGAGA ATTNCTCACG ANNCCTGAAN   961 TGGAAGAAAA AAANTTTGAN NCNCTGNNTG ANGNTTGANN ATGNANNNNN NATCNNAACT 1021 CNAAANNGNN NNNTNNNNNN ANANTTNCNN NNNNNNNN //
Primer: hGCSF_R (Pr0031) Region: hG-CSF 3'UTR+pA,IgG-Fc-region Sequence file: RDB18986_B1Drc.seq
>D07990C5_B1Dr_2_hGCSF_R_B04_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCCCNG GTGGGGNNCC TCACTCTAGA GTCGCGGCCG    61 CTCTAGGGCA GTGGTGGGTG CTTTATTTCC ATGCTGGGTG CCTGGGAAGT ATGTAGACGG   121 GGTACGTGCC AAGCATCCTC GTGCGACCGC GAGAGCCCGG GGAGCGGGGG CTTGCCGGCC   181 GTCGCACTCA TTTACCCGGG GACAGGGAGA GGCTCTTCTG CGTGTAGTGG TTGTGCAGAG   241 CCTCATGCAT CACGGAGCAT GAGAAGACGT TCCCCTGCTG CCACCTGCTC TTGTCCACGG   301 TGAGCTTGCT ATAGAGGAAG AAGGAGCCGT CGGAGTCCAG CACGGGAGGC GTGGTCTTGT   361 AGTTGTTCTC CGGCTGCCCA TTGCTCTCCC ACTCCACGGC GATGTCGCTG GGATAGAAGC   421 CTTTGACCAG GCAGGTCAGG CTGACCTGGT TCTTGGTCAT CTCCTCCCGG GATGGGGGCA   481 GGGTGTACAC CTGTGGTTCT CGGGGCTGCC CTGTAGGGAC AGAGGTTGGT ACAGCGGTCA   541 CTCTCAGGGC AGAGGGTGGG CCGAGCCGGC CTCTGTCCAT GTGGCCCTCG CACCCCACGG   601 GTCCCACCTT TGGCTTTGGA GATGGTTTTC TCGATGGGGG CTGGGAGGGC TTTGTTGGAG   661 ACCTTGCACT TGTACTCCTT GCCATTCAGC CAGTCCTGGT GCAGGACGGT GAGGACGCTG   721 ACCACACGGT ACGTGCTGTT GTACTGCTCC TCCCGCGGCT TTGTCTTGGC NTTATGCACC   781 TCCACGCCGT CCACGTACCA GTTGAACTTG ACCTCAGGGT CTTCGTGGCT CACGTCCACC   841 ACCACGCATG TGACCTCANG GGTCNGGGAN ATCATGANGG TGTCCTTGGG TTTTGGGGGG   901 GAAGAGGAAG ANTGNNGGTC CCCCNNNGAN TNNAGGTGCT GANGAAGAGA TGGAGGTGGA   961 NGTGTCAGCA CCCNGNNTGG GGCCTGNNCC TGGATGCNNN NNANTCTNGN NNNNNNNNCC  1021 NNCNNNGANC NNGNNNGNCG ANNNNTNNNN GGNNTNACNN NNGNNNNNNN GNNNNNNNNN 1081 N //
Primer: Fc-CH2M_R (Pr1251) Region: IgG Fc-region Sequence file: RDB18986_B1Drd.seq
>D07990C5_B1Dr_2_Fc-CH2M_R_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NNNTGNNNNC ACGGTACGTG CTGTTGTNNC    61 TGCTCCTCCC GCGGCTTTGT CTTGGCATTA TGCACCTCCA CGCCGTCCAC GTACCAGTTG   121 AACTTGACCT CAGGGTCTTC GTGGCTCACG TCCACCACCA CGCATGTGAC CTCAGGGGTC   181 CGGGAGATCA TGAGGGTGTC CTTGGGTTTT GGGGGGAAGA GGAAGACTGA CGGTCCCCCC   241 AGGAGTTCAG GTGCTGAGGA AGAGATGGAG GTGGACGTGT CAGCACCCGG CTGGGGCCTG   301 TCCCTGGATG CAGGCTACTC TAGGGCACCT GTCCCGCCTT GAGCTGGAGG GCGAGGCCTG   361 GGCTGGCTTA CCTGGGCACG GTGGGCATGT GTGAGTTTTG TCACAAGATT TGGGCTCTGC   421 AGAGAGAAGA TTGGGAGTTA CTGGAATCTG GGAGGAGAGA AGGTGTCCGA GCTGAGGGAG   481 TGGAGAGTTT GGCCTTTGGG GTGGGCTTAG GTCAGGGGCA GGGTCCTCCC GGATATGGCT   541 CTTGGCAGGT CTGAGCCCAG CACCTGCCCC TTTGTGTGCA GGGCCTGGGT TAGGGGCACC   601 TAGCCTGTGC CTGCCCAGAG CCTGGGGAAA AAGCCAGAAG ACCCTCTCCC TGAGCATGAG   661 TGGGGCGGGC AGAGGCCTCC GGGTGAANAN GCAGACGGGG CCTGCCTTGC TGCCCTGGAC   721 TGGGACTGCA TAGCCGGGAT GCGTCCNGNN NNGATAGAAC TAGTGGATCC CCCGGGCTGN   781 NNGAATTCGA TATCGACTTA CTAGTGTGAT TACTCCAGGT ATCTCCTGAN GATGTCNCAG   841 TGCCATTCCA GTCTGGGTTT GTGAGTCCNT GTGTGTGTAG ATGTNNGANG AGTGGANGTA   901 NANACTCTGG GTTGATGTTN GGTNCATGTG NNGNATCTTG TTGAAATANT NNNTGGGGNC   961 NTNCNTGTNN NTGTGGGCNN TGNNANTTNN TGGGGTCNTT CNTNGGANGA NNTGTGGGNA  1021 NTCTCNGGGT TTNNNNNTNN NNANGAAAAN GNTCNCNTTT NNNNATNNNT TAAANCNNNN 1081 NCNNNGNANT NNNNCNNCNN NNNNNNNN //
Primer: EF1a-N_R (Pr0024) Region: SV40 pro_ori,lacO,lac pro Sequence file: RDB18986_B1Dre.seq
>D07990C5_B1Dr_2_EF1a-N_R_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGCGNNGTG CGCTCTGCCC NCTGACGGGC ACCGGANCCN    61 ATTCCCACTC CTTTCAAGAC CTAGAAGGTC CATTAGCTGC AAAGATTCCT CTCTGTTTAA   121 AACTTTATCC ATCTTTGCAA AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC   181 ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA   241 TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG   301 GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC   361 CTGCTGGGGA GGGAATTAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT   421 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG   481 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG   541 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT   601 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCNNTC GGTCGTTCGG   661 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG   721 GANAACGCAG GAAAGAACAT GTGAGCAAAA GGNCANCAAA AGGNCAGGAA CCGTAAAAAG   781 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAANTCGA   841 CGCTCNNNTC ANAGGTGGCN AAANCCGACA GGANTATAAN NATANNAGGC GTTTCCCCCT   901 GGAAGCTCCC TCGTGCGNTC NCCNGTTCCN ANCCTGNCNC TTNNCNGGAT ACNNGNNCNC   961 CTTTCNNCCC NTNNGNAANC GTNNNGCTTT CTNATANNNC NCGCTGNNNN NATNTNNNNN 1021 NNNGNNGTNN NNNNNTCNNN NNANNNNNGG NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

GNP_filter3_RDBDEP_html_231001.pl