Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCX4-hBSG-c-HA (269)

Retroviral expression vector of human BSG.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Suzuki, J. et al., Proc. Natl. Acad. Sci. USA., 113 (34): 9509–9514, 2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Suzuki, J. et al., Proc. Natl. Acad. Sci. USA., 113 (34): 9509–9514, 2016)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18966_B1Dkp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: Pol region,insert 5' Sequence file: RDB18966_B1Dka.seq
>D07990A6_B1Dk_1_MMLV-pol_F_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG NATCGCAGCT TGGNNACNCG CCGCCCACGT NGAAGGCTGC    61 CGACCCCGGG GGTGGNNCAT CCTCTAGACT GCCGGATCCA TGGCGGCTGC GCTGTTCGTG   121 CTGCTGGGAT TCGCGCTGCT GGGCACCCAC GGAGCCTCCG GGGCTGCCGG CACAGTCTTC   181 ACTACCGTAG AAGACCTTGG CTCCAAGATA CTCCTCACCT GCTCCTTGAA TGACAGCGCC   241 ACAGAGGTCA CAGGGCACCG CTGGCTGAAG GGGGGCGTGG TGCTGAAGGA GGACGCGCTG   301 CCCGGCCAGA AAACGGAGTT CAAGGTGGAC TCCGACGACC AGTGGGGAGA GTACTCCTGC   361 GTCTTCCTCC CCGAGCCCAT GGGCACGGCC AACATCCAGC TCCACGGGCC TCCCAGAGTG   421 AAGGCTGTGA AGTCGTCAGA ACACATCAAC GAGGGGGAGA CGGCCATGCT GGTCTGCAAG   481 TCAGAGTCCG TGCCACCTGT CACTGACTGG GCCTGGTACA AGATCACTGA CTCTGAGGAC   541 AAGGCCCTCA TGAACGGCTC CGAGAGCAGG TTCTTCGTGA GTTCCTCGCA GGGCCGGTCA   601 GAGCTACACA TTGAGAACCT GAACATGGAG GCCGACCCCG GCCAGTACCG GTGCAACGGC   661 ACCAGCTCCA AGGGCTCCGA CCAGGCCATC ATCACGCTCC GCGTGCGCAG CCACCTGGCC   721 GCCCTCTGGC CCTTCCTGGG CATCGTGGCT GANGTGCTGG TGCTGGTCAC CATCATCTTC   781 ATCTACGANA AGCGCCGGAA GCCCGANGAC GTCCTGGATG ATGACGACGC CNGCTCTGCA   841 CCCCTGAAGA NCAGCGGGCA GCACCANAAT GACAAAGGCA AGAACGTCCG CCAGANGAAC   901 TCTTNCNAAN TCNTGTACCC NTACNATGTT CCANATTANN CTNGANTCNN GCGGNNNCNG   961 NNNNNNTTGN NANCTNACNN NNNCTANCNN CNGNNTTNCN NNNANCNGNN NNNNNNNCNN 1021 NNNNNNNN //
Primer: IRES-R (Pr0037) Region: IRES,insert 3'(HA,hBSG 3') Sequence file: RDB18966_B1Dkb.seq
>D07990A6_B1Dk_1_IRES-R_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTNA GGGGGGGGGG GCGGANTTGA TCCCGCTAGA    61 GGGAAACCGT TGCTAGCTTA AGTTAGTTAA CAATTGTTAC CGCGGCCGCT CGAGTCAAGC   121 GTAATCTGGA ACATCGTATG GGTACATGAA TTCGGAAGAG TTCCTCTGGC GGACGTTCTT   181 GCCTTTGTCA TTCTGGTGCT GCCCGCTGCT CTTCAGGGGT GCAGAGCCGG CGTCGTCATC   241 ATCCAGGACG TCCTCGGGCT TCCGGCGCTT CTCGTANATG AAGATGATGG TGACCAGCAC   301 CAGCACCTCA GCCACGATGC CCAGGAAGGG CCAGAGGGCG GCCAGGTGGC TGCGCACGCG   361 GAGCGTGATG ATGGCCTGGT CGGAGCCCTT GGAGCTGGTG CCGTTGCACC GGTACTGGCC   421 GGGGTCGGCC TCCATGTTCA GGTTCTCAAT GTGTAGCTCT GACCGGCCCT GCGAGGAACT   481 CACGAANAAC CTGCTCTCGG AGCCGTTCAT GAGGGCCTTG TCCTCANAGT CAGTGATCTT   541 GTACCAGGCC CAGTCAGTGA CAGGTGGCAC GGACTCTGAC TTGCANACCA GCATGGCCGT   601 CTCCCCCTCG TTGATGTGTT CTGACGACTT CACAGCCTTC ACTCTGGGAG GCCCGTGGAG   661 CTGGATGTTG GCCGTGCCCA TGGGCTCGGG GAGGAAGACG CAGGAGTACT CTCCCCACTG   721 GTCGTCGGAG TCCACCTTGA ACTCCGTTTT CTGGCCGGGC AGCGCGTCCT CCTTCAGCAC   781 NACGCCCCCC TTCAGCCAGC GGTGCCCTGT GANCTCTGTG GCGCTGTCAT TCAAGGAGCA   841 GGTGANGAGT ATCTNGNANC CAAGGTCTTC TACGGNAGTG ANNANTGTGC CGGNAGCCCC   901 NGANGNTCCG NGGGNGNCCA GCAGCGCGAA TCCNNGCANC ACGAACAGCG CANCCNCNNT   961 NNNNNCNGNN AGNCTANANN NNGGNCNNCC CCNNGGGNNN NNNNNCNTNN NNNNNGNNNN 1021 NNNNNN //
Primer: bsr-C_F (Pr0803) Region: 3' LTR Sequence file: RDB18966_B1Dkc.seq
>D07990A6_B1Dk_1_bsr-C_F_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NNNGTTNNTN NNNNNNNNNN GCANNNNNNT    61 CAAAACTACG ATTGAAGAAC TCATTCCACT CAAATATACC CGAAATTAAA AGTCGACGAT   121 AAAATAANNN NNNTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG   181 GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAAATA CATAACTGAG   241 AATAGAGAAG TTCAGATCAA GGTCAGGAAC AGATGGAACA GCTGAATATG GGCCAAACAG   301 GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG GAACAGCTGA   361 ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC   421 AGATGGTCCC CAGATGCGGT CCAGCCCTCA GCAGTTTCTA GAGAACCATC AGATGTTTCC   481 AGGGTGCCCC AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA ATCAGTTCGC   541 TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC CACAACCCCT   601 CACTCGGGGC GCCAGTCCTC CGATTGACTG AGTCGCCCGG GTACCCGTGT ATCCAATAAA   661 CCCTCTTGCA GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG   721 TGATTGACTA CCCGTCAGCG GGGGTCTTTC NCATGCAGCA TGTATCAAAA TTAATTTGGT   781 TTTTTTTCTT AAGNATTTAC ATTAAATGGN CATAGTTGCA NTAATGAATC GGCCNACGCG   841 CGGGGAGAGG CGGNTTGCGT ATTGGGCGCT CTTCCGCTNN CTCGCTCACN GACTCGCTGC   901 GCTCGGNNGT TCGGCTGCGN CGAGCGGNAT CNGCTCACTN AANGNNGNNA ATACNNNNNN   961 TCCNCANAAT CNNGNGNNNN CGCNNNNAAG ANNNTGNNNN NNNANNNNNN GNNANNNNNN 1021 NGNNNCNNNN AANNNCNNNN NNGNNGGCGT TTTTNNNNNN NGNNNNNNNN NN //
Primer: CMV enh_F1 (Pr0652) Region: CMV en,5' LTR Sequence file: RDB18966_B1Dkd.seq
>18966_18966_B3FfF_1_CMV enh_F1_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNTNNNG CNNNGTANNT GNNCTTATGG GACTTTCCTA CTTGGCAGTA    61 CATCTACGTA TTAGTCATCG CTATTACCAT GCTAGAGAAC CATCAGATGT TTCCAGGGTG   121 CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG   181 CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG   241 GCGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT   301 TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG 361 ACTACCCGTC AGCGGGGGTC TTTCATTTGG GGGNTCNNNN NNNNNN //
Primer: pMFG_1 (Pr0230) Region: Pol region,gag(truncated) Sequence file: RDB18966_B1Dke.seq
>18966_18966_B3FfF_1_pMFG_1_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGTN NNNNNNTCTT GTCNNGTCNG GGTCNGGGAA GTTTACAAGG    61 GGCGGGGGTG CCCCATATAA GATCTCATAT GGCCATATGG GGGCGCCTAG AGAAGGAGTG   121 AGGGCTGGAT AAAGGGAGGA TCGAGGCGGG GTCGAACGAG GAGGTTCAAG GGGGAGAGAC   181 GGGGCGGATG GAGGAAGAGG AGGCGGAGGC TTAGGGTGTA CAAAGGGCTT GACCCAGGGA   241 GGGGGGTCAA AAGCCAAGGC TTCCCAGGTC ACGATGTAGG GGACCTGGTC TGGGTGTCCA   301 TGCGGGCCAG GTGAAAAGAC CTTGATCTTA ACCTGGGTGA TGAGGTCTCG GTTAAAGGTG   361 CCGTCTCGCG GCCATCCGAC GTTAAAGGTT GGCCATTCTG CAGAGCAGAA GGTAACCCAA   421 CGTCTCTTCT TGACATCTAC CGACTGGTTG TGAGCGATCC GCTCGACATC TTTCCAGTGA   481 CCTAAGGTCA AACTTAAGGG AGTGGTAANN NNCNGGCCCT AATTTTNNNA NNAATACAGA   541 AACACNNTCN NACNNANACA ACACAGAACG ATGCTGCANC AGACAAGANN NNNGGCGCGG 601 CTTCGGNCNN NAACCGAAAG NNNNAANTNN NANNGANNNN NNNNNN //
Primer: IRES-F (Pr0036) Region: IRES,bsr,3' LTR Sequence file: RDB18966_B1Dkf.seq
>D07990A6_B1Dk_1_IRES-F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGTCGANGTN NNNNNNNNGT CTAGGCCCCC CGAACCNCGG    61 GGACGTGGTT TTCCTTTGAA AAACACGATA ATACCATGGT CATGAAAACA TTTAACATTT   121 CTCAACAAGA TCTAGAATTA GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG   181 ATAATAAACA TCATGTGGGA GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG   241 TACATATTGA AGCGTATATA GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA   301 GTGCAGTTTC GAATGGACAA AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT   361 CTGACGAAGT AGATAGAAGT ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA   421 TTTCAGACTA TGCACCAGAT TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA   481 CTACGATTGA AGAACTCATT CCACTCAAAT ATACCCGAAA TTAAAAGTCG ACGATAAAAT   541 AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG   601 GCAAGCTAGC TTAAGTAACG CCATTTTGCA AGGCATGGAA AAATACATAA CTGAGAATAG   661 AGAAGTTCAG ATCAAGGTCA GGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT   721 CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGAACA GCTGANTATG   781 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCN AGAACAGATG   841 GTCCCCAGAT GCGGTCCAGC CCTCAGCAGT TTCTAGAGAN NCATCAGATG TTTCCAGNNN   901 GCCCCANNGA CCTGNAATGA NCCTGNGCCT TANTTGAACT AACCNATCAG TTCGCTTCTC   961 NCNTCTGNNC GCGCGNNTCT GNNNCCNNAN CNNNNTAAAA NAGNCNNNNN NCNNNNNNNN 1021 NGGGGNNNNN NNNNN //
Primer: MMLV psi_F (Pr0863) Region: MMLV psi,gag(truncated),pol region Sequence file: RDB18966_B1Dkg.seq
>D07990A6_B1Dk_1_MMLV psi_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNC NNNNNTTTGN TTTCGGTTTG GGANCGAAGC CGCGCCGCGC    61 GTCTTGTCTG CTGCAGCATC GTTCTGTGTT GTCTCTGTCT GACTGTGTTT CTGTATTTGT   121 CTGAAAATTA GGGCCAGACT GTTACCACTC CCTTAAGTTT GACCTTAGGT CACTGGAAAG   181 ATGTCGAGCG GATCGCTCAC AACCAGTCGG TAGATGTCAA GAAGAGACGT TGGGTTACCT   241 TCTGCTCTGC AGAATGGCCA ACCTTTAACG TCGGATGGCC GCGAGACGGC ACCTTTAACC   301 GAGACCTCAT CACCCAGGTT AAGATCAAGG TCTTTTCACC TGGCCCGCAT GGACACCCAG   361 ACCAGGTCCC CTACATCGTG ACCTGGGAAG CCTTGGCTTT TGACCCCCCT CCCTGGGTCA   421 AGCCCTTTGT ACACCCTAAG CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC   481 TTGAACCTCC TCGTTCGACC CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC   541 TAGGCGCCCC CATATGGCCA TATGAGATCT TATATGGGGC ACCCCCGCCC CTTGTAAACT   601 TCCCTGACCC TGACTTGACA AGAGTTACTA ACAGCCCCTC TCTCCAAGCT CACTTACAGG   661 CTCTCTACTT AGTCCAGCAC GAAGTCTGGA GACCTCTGGC GGCAGCCTAC CAAGAACAAC   721 TGGACCGACC GGTGGTACCT CACCCTTACC GAGTCGGCGA CACAGTGTGG GTCCGCCGAC   781 ACCAGACTAA GAACCTAGAA NCTCGCTGGA AAGGANCTTA CACAGTCCTG CTGACCACCC   841 CCACCGCCCT CAAAGTAGAC GGCATCGCAG CTTGNATACA CGCCNCCCAC NNGAANGCTG   901 NCNANCCNNG GGGNGGNNNA TCCTCTANAN TGNCNGANCC NTNGNGGCTG CGCTNNNCNN 961 GCTGCNNGGN NTNGCGCTGN NGGGNNNCNN NNNNNCNNNN NGGGGCTGNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

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