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pMXs-AMNN-Puro-mCherry-CTSB (#RDB18953)

Retroviral expression vector of mouse cathepsin B (CTSB).

Clone info. Retroviral expression vector of mouse cathepsin B (CTSB), mCherry tagged with N-terrminaus of CTSB.
Comment Expression was confirmed by the depositor with immunofluorescence analysis.
Vector backbone pMXs-AMNN-puro (plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Gene/insert name mouse CTSB cDNA
Depositor|Developer Higami, Yoshikazu |
 
Sequence (full) RDB18953zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Mizunoe, Y. et al., Sci. reports, 10 (1): 634, 2020).
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mizunoe, Y. et al., Sci. reports, 10 (1): 634, 2020)。
MTAに書く付加的使用条件:
pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18953 pMXs-AMNN-Puro-mCherry-CTSB Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMXs-AMNN-Puro-mCherry-CTSB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18953).

Reference section:

Mizunoe, Y., Kobayashi, M., Hoshino, S., Tagawa, R., Itagawa, R., Hoshino, A., Okita, N., Sudo, Y., Nakagawa, Y., Shimano, H., Higami, Y., Cathepsin B overexpression induces degradation of perilipin 1 to cause lipid metabolism dysfunction in adipocytes. Sci. Rep. 10 (1): 634 (2020). PMID 31959889. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Mizunoe, Y., Cathepsin B overexpression induces degradation of perilipin 1 to cause lipid metabolism dysfunction in adipocytes. Sci. Rep. 10 (1): 634 (2020). PMID 31959889. [link to RRC of NBRP]