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pGBD-C1-Rab29-deltaCys-Q67L (#RDB18888)

Expression vector of mouse Rab29 constitutive active mutant (Q67L) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.

Clone info. Expression vector of mouse Rab29 constitutive active mutant (Q67L) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
Comment Expression was confirmed by the depositor with yeast-two hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin (E. coli), TRP1 (yeast)
Gene/insert name mouse Rab29 cDNA
Reference of insert sequence AB232625 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Fukuda, M. J. Biol. Chem., 278 (17): 15373-15380, 2003); (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006).
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. J. Biol. Chem., 278 (17): 15373-15380, 2003); (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006)。

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RDB18888 pGBD-C1-Rab29-deltaCys-Q67L Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pGBD-C1-Rab29-deltaCys-Q67L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18888).

Reference section:

Itoh, T., Satoh, M., Kanno, E., Fukuda, M., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]