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pGBD-C1-Rab27A-deltaCys(C119/C121A)-N133I

Expression vector of mouse Rab27A constitutive negative mutant (C119/C121A and N133I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.

Catalog number RDB18883
Resource name pGBD-C1-Rab27A-deltaCys(C119/C121A)-N133I
Clone info. Expression vector of mouse Rab27A constitutive negative mutant (C119/C121A and N133I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
Comment Expression was confirmed by the depositor with yeast-two hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin (E. coli), TRP1 (yeast)
Gene/insert name mouse Rab27a cDNA
Reference of insert sequence AB232622 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab27a

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Kuroda, T. S. et al., J. Biol. Chem., 277 (11): 9212-9218, 2002); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al., J. Biol. Chem., 277 (11): 9212-9218, 2002); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。
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          RDB18883 pGBD-C1-Rab27A-deltaCys(C119/C121A)-N133I Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.
          reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          2022.05.16

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