Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGBD-C1-Rab22B-deltaCys-N118I

Expression vector of mouse Rab22B constitutive negative mutant (N118I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.

Catalog number RDB18873
Resource name pGBD-C1-Rab22B-deltaCys-N118I
Clone info. Expression vector of mouse Rab22B constitutive negative mutant (N118I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay. Rab31 in NCBI.
Comment Expression was confirmed by the depositor with yeast-two hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin (E. coli), TRP1 (yeast)
Gene/insert name mouse Rab31 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse Rab31
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18873 pGBD-C1-Rab22B-deltaCys-N118I Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.
          reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

          2022.05.16

          GNP_filter3_RDBDEP_html_220516.pl