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pGBD-C1-Rab22B-deltaCys-N118I (#RDB18873)

Expression vector of mouse Rab22B constitutive negative mutant (N118I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Osaki, F., J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse Rab22B constitutive negative mutant (N118I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay. Rab31 in NCBI.
Comment Expression was confirmed by the depositor with yeast-two hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin (E. coli), TRP1 (yeast)
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Rab31 cDNA
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。

Catalog # Resource name Shipping form Fee
RDB18873 pGBD-C1-Rab22B-deltaCys-N118I DNA solution

How to cite this biological resource

Materials & Methods section:

The pGBD-C1-Rab22B-deltaCys-N118I was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18873).

Reference section:

Osaki, F., Matsui, T., Hiragi, S., Homma, Y., Fukuda, M., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [PubMed] [Article] [RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [PubMed] [Article] [RRC of NBRP]