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pGBD-C1-Rab6D-deltaCys-Q89L

Expression vector of human Rab6D constitutive active mutant (Q89L) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.

Catalog number RDB18834
Resource name pGBD-C1-Rab6D-deltaCys-Q89L
Clone info. Expression vector of human Rab6D constitutive active mutant (Q89L) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay. Rab41 in NCBI, one AA longer than that in NCBI.
Comment Expression was confirmed by the depositor with yeast-two hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin (E. coli), TRP1 (yeast)
Gene/insert name human RAB41 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
human RAB41
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Aizawa, M. & Fukuda, M. J. Biol. Chem., 290 (36): 22250-22261, 2015); (Ohishi, Y. et al., J. Biol. Chem. 294 (17): 6912-6922, 2019).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Aizawa, M. & Fukuda, M. J. Biol. Chem., 290 (36): 22250-22261, 2015); (Ohishi, Y. et al., J. Biol. Chem., 294 (17): 6912-6922, 2019)。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB18834 pGBD-C1-Rab6D-deltaCys-Q89L Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Ohishi, Y., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Ohishi, Y., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268.
          reference Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634.

          2022.05.16

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