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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

human ATP11A (#RDB18736)

Retroviral expression vector of human ATP11A.

Clone info. Retroviral expression vector of human ATPase phospholipid transporting 11A (ATP11A) tagged with FLAG at C-terminus.
Comment derivation：ATP11A, sKBM7 cell [M. Kotecki, et al. Exp. Cell Res. 252, 273-280 (1999)]. Expression was confirmed by the depositor with microscopy.
Vector backbone pMXs-puro (plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Gene/insert name human ATP11A cDNA
Depositor|Developer Nagata, Shigekazu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Segawa, K. et al., Science, 344 (6188): 1164-1168, 2014); (Segawa, K. et al., J. Biol. Chem., 291 (2): 762-772, 2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引 用する (Segawa, K. et al., Science, 344 (6188): 1164-1168, 2014); (Segawa, K. et al., J. Biol. Chem., 291 (2): 762-772, 2016)。 利用者が教育・学術研究以外の目的で用いる場合、大阪大学と 別途 MTA を締結すること。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18736 human ATP11A DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human ATP11A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18736).

Reference section:

Segawa, K., Kurata, S., Nagata, S., Human Type IV P-type ATPases That Work as Plasma Membrane Phospholipid Flippases and Their Regulation by Caspase and Calcium. J. Biol. Chem. 291 (2): 762-772 (2016). PMID 26567335. [link to RRC of NBRP]
Segawa, K., Kurata, S., Yanagihashi, Y., Brummelkamp, T.R., Matsuda, F., Nagata, S., Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure. Science 344 (6188): 1164-1168 (2014). PMID 24904167. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18736_B1C2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(hATP11A 5')
Sequence file: RDB18736_B1C2a.seq check
>D07512B3_B1C2_2_MMLV-pol_F_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA TCGNANCTTG GNNACNCGCC GCCCACGTGA AGGCTGCCGA
   61 CCCCGGGGGT GGANCATCCT CTAGACTGCC GGATCTAGCT AGTTAATTAA GGAGGAGCCA
  121 TGGACTGCAG CCTCGTGCGG ACGCTCGTGC ACAGATACTG TGCAGGAGAA GAGAATTGGG
  181 TGGACAGCAG GACCATCTAC GTGGGACACA GGGAGCCACC TCCGGGCGCA GAGGCCTACA
  241 TCCCACAGAG ATACCCAGAC AACAGGATCG TCTCGTCCAA GTACACATTT TGGAACTTTA
  301 TACCCAAGAA TTTATTTGAA CAATTCAGAA GAGTAGCCAA CTTTTATTTC CTTATCATAT
  361 TTCTGGTGCA GTTGATTATT GATACACCCA CAAGTCCAGT GACAAGCGGA CTTCCACTCT
  421 TCTTTGTCAT TACTGTGACG GCTATCAAAC AGGGTTATGA AGACTGGCTT CGACATAAAG
  481 CAGACAATGC CATGAACCAG TGTCCTGTTC ATTTCATTCA GCACGGCAAG CTCGTTCGGA
  541 AACAAAGTCG AAAGCTGCGA GTTGGGGACA TTGTCATGGT TAAGGAGGAC GAGACCTTTC
  601 CCTGCGACTT GATCTTCCTT TCCAGCAACC GGGGAGATGG GACGTGCCAC GTCACCACCG
  661 CCAGCTTGGA TGGAGAATCC AGCCATAAAA CGCATTACGC GGTCCAGGAC ACCAAAGGCT
  721 TCCACACAGA NGANGATATC GGCGGANTTC ACGCCACCAT CGAGTGTGAG CAGCCCCAGC
  781 CCGACCTCTA CNAGTTCGTG GGTCGCATCA ACGTTTACAG TGNCCNGAAT GANCCCGNGG
  841 TGAGGNCCTT ANGATCGGAA AACCTGCTGC TTANANGANC TACNCNGAAG AACACTGANA
  901 AAATCTTNNG NGTGGCNANT NACNCGGGAA NNNAAANNNN NNNNGNNTTN NNTTATCNNN
961 NNAANNCTCN NANNNNNTNN GNNNNNNNNA AANNNNNNNN NN
//
Primer: SV40pro_R2 (Pr0516)
Region: SV40 pro_ori,insert 3'(EGFP 3')
Sequence file: RDB18736_B1C2b.seq check
>D07512B3_B1C2_2_SV40pro_R2_B09_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT CTGCCTGCTG GGGNNCCTGG GGNNTTTCCN CACCCTAACT
   61 GACACACATT CCACAGGTCG ACCACTGTGC TGGCGGCCGC TCGAGTTACT TGTACAGCTC
  121 GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG
  181 CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG
  241 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT
  301 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC
  361 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
  421 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
  481 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
  541 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
  601 GTAGCGGCTG AAGCACTGCA CGCCGTAgGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
  661 GGGCAGCTTG CCGGTGGTGC ANATGAACTT CANGGTCAGC TTGCCGNNNG TGGCATCGCC
  721 CTCGCCCTCG CCGGACACGC TGAACTTGTG NNCGTTTACG TCGCCGTCCN GCTCGACCNG
  781 GNNGGGCACC ACCCCNGNGA ACAGCTCCTC GCCCTTGNTC ACNNNNTCNA AACTCNNNNG
841 CTNGNANNCT GANAAAGCAT AAANNNGGNN NANNGNNCNA CNNAANNGNN NNNNNNNN
//
Primer: EGFP-N_R2 (Pr0905)
Region: insert mid(EGFP 5',hATP11A 3')
Sequence file: RDB18736_B1C2c.seq check
>D07512B3_B1C2_2_EGFP-N_R2_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNANNNNNGN NNGCCNNANG TCAGGGNGGT CACGAGGGTG
   61 GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG
  121 GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC
  181 AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACGAATTCG
  241 AAACTCAGGC TGCTGGAAGT CTGAGAAAGC ATAAAGATGG TTGACTGCTC GACAGAAAGG
  301 CACTGGCTCT TAGTCTGGAC TCTCTCTGTT GCTGTTGGCC ACAGCTGCCG GCACAGGACT
  361 TTCTTGAGGA TGTCGGGAAG GAGGCTGATG GTCACCAGCA GCACGATGGC CAGCCAGGCG
  421 GGCCCGCTGG ACAGCATCTG GATGAACACG TAGTACATCC TCTGGTAGTT GAGGAACGGC
  481 CAGATCACTC CTCCCCAGAG AAGCGAAAAG ACAACGTAGA ACAGCAGCGA CCCCCAGATG
  541 ACAAAATGGT TGATCCAAGT CCAGTAGTGT GTGTCCAATG CAAGCTTTAG TGTAACTGTG
  601 AACACCATCA CGGTGAATAC CAGCGTTCCA AACGTCCAGT TTCCAAATAT CTGCCCGTTG
  661 CTTGTCACAG TTGTATTTTC AAACACGAAA TAAGCACCAA AGAAGAACAC CAGTGCGTCA
  721 AACAGTCCCA GGAGCGTCCA GTAGATGAAC ACGCGCCAGC GCAGCANGGC ATTCTTGGCG
  781 ACGTCCCTGT ACAGGNNCGG GTCTCTCTTG ANCACGTCAA TGCCAACATG CTGCTCCATG
  841 ANGCTGTACA GGANGNTGGG GGAGGGANGN GANNCTGATG TTGNAGANGG TCANATACNC
  901 GGNGNCGTAN AAAGTCTGNT GTGAANNNCC NNAGANNAAN TGGTATAAAA ACTGNNGNNA
961 GNNGNNNNAN ANNNNCNNNN NNNANNANNT NNNGNNNNNN NNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,puroR
Sequence file: RDB18736_B1C2d.seq check
>D07512B3_B1C2_2_SV40pro_ori_F_D09_12_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNGCC TCGGCCTCTG ANCTNTTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTTA CCATGACCGA GTACAAGCCC ACGGTGCGCC
  121 TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT
  181 ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC
  241 AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG
  301 GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG
  361 AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG
  421 AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG
  481 TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG
  541 CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT
  601 TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA
  661 CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG CAGCGCCCGA
  721 CCGAAAGGAG CGCACGACCC CATGCATCGT CGACGATAAA ATAAAAGATT TTATTTAGTC
  781 TCCAGAAAAA GGGGGGAATG AAAGANNNCA CCTGTANNTT TGGCAAGCTA GCTTAAGTAA
  841 CGCCATTTTG CAAGGNATGG AAAAATACAT AACTGANAAT AGAGAAGTTC AGATCAAGGT
  901 CAGGAACAGA NGGAACAGNT NAANATNNGC CAAACAGGNN ATCTGNNNNA AGCANNNNCN
961 NNCCCNGNNC NNNNNCNNNN NNNNGNNGNN NNNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,5' LTR
Sequence file: RDB18736_B1C2e.seq check
>D07512B3_B1C2_2_Amp_R_E09_15_ABI24.ab1
    1 NNNNNNNNNN NNNANTGNNN CANTNATNNG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
  601 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC
  661 GCTGTTCCTT GGGAGGGTCT CCTCTGANTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT
  721 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGN CCACCGACCC ACCACCGGGA
  781 GGTAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCNATTGT CTAGTGTCTA TGACTGATTT
  841 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGNGGA NCCGNGGNGN
  901 AACTGACGAN TTNNGNANNN CCGGNCGCNA CCCNGGGANA NGTCCCNNNN NNNTCGGGGG
961 GNNGNTTTTT GNNNNNNNNN N
//
Primer: pMFG_1 (Pr0230)
Region: pol region,gag(truncated),MMLV psi
Sequence file: RDB18736_B1C2f.seq check
>D07512B3_B1C2_2_pMFG_1_F09_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNNN NNNNTCTTGT NNTGTNNGGG TCAGGGAAGT TTACAAGGGG
   61 CGGGGGTGCC CCATATAAGA TCTCATATGG CCATATGGGG GCGCCTAGAG AAGGAGTGAG
  121 GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG GGAGAGACGG
  181 GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA CCCAGGGAGG
  241 GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG GGTGTCCATG
  301 CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT TAAAGGTGCC
  361 GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG TAACCCAACG
  421 TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT TCCAGTGACC
  481 TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCTAA TTTTCAGACA AATACAGAAA
  541 CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCAG ACAAGACGCG CGGCGCGGCT
  601 TCGGTCCCAA ACCGAAAGCA AAAATTCAGA CGGAGGCGGG AACTGTTTTA GGTTCTCGTC
  661 TCCTACCAGA ACCACATATC CCTCCTATTA NGGGGNTGCA CCAAAGAGTC CAAAACGATC
  721 GGGATTTTTG GACTCAGGTC GGGCCACAAA AACGGCCCCC GAAGTCCCTG GGANGTCTCC
  781 CAGGGTTGCG NCCGGGTGTT CCGAACTCGT CAGTTCCACC ACGGGTCCGC CAGATACAGA
  841 NCTAGTTAGC TAACTAGTAC CGACGCANGC GCATAAAATC AGTCATAGAC NCTAGACAAT
  901 CNGANAGANA CAGATNAGTT GCTNGCCAGC TTACCNNCNN GNGGTGGGNC GGNGGTCCCN
  961 NGGNAGGGNN NNNCCNNTNC NNGNANNANC CCCNNNNGNA NNNNCNCNNN NNNNNNGNNN
1021 NNNNANNNNN NNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB18736_B1C2g.seq check
>D07512B3_B1C2_2_lacIpro-B_F_G09_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGNN NCGNNNCNGT GANCGAGGAA GCGGAAGANC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA
  121 AATACTTAAG AAAAAAAACC AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC
  181 TGACGGGTAG TCAATCACTC AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG
  241 ATGCAACTGC AAGAGGGTTT ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG
  301 GACTGGCGCC CCGAGTGAGG GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC
  361 GCGAACAGAA GCGAGAAGCG AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA
  421 GGTCCTTGGG GCACCCTGGA AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC
  481 GCATCTGGGG ACCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA
  541 TCCTGTTTGG CCCATATTCA GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC
  601 TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG
  661 ATCTGAACTT CTCTATTCTC AGTTATGTAT TTTTCCATGC CTTGCAAAAT GGCGTTACTT
  721 AAGCTAGCTT GCCAAACCTA CAGGTGGGGT CTTTCATTCC CCCCTTTTTC TGGAGACTAA
  781 ATAAAATCTT TTANTTTATC GTCGACGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC
  841 GCTGCGGGTC GTGGGGCGGG CGTCANGCAC CNGGCTNGNG GGTCATGCAC CANGTGCGCG
  901 GNCCTTCNGG CNNCTCGNCG TCNNCGGNGA CGGNGAAGCC NANCNNCTCG NANAANNGGN
  961 NNGNNGNGGG GCGCNNNNNN NNCCANNNNN NNGGNNNNCC NNGNNNNNNN NGNNNNNNNN
1021 NN
//
Primer: hATP11A_F1 (Pr1112)
Region: insert mid(hATP11A mid)
Sequence file: RDB18736_B1C2h.seq check
>D07512B3_B1C2_2_hATP11A_F1_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTTNNNNNNG GAAAANCTGC TGCTTAGAGG
   61 GNAGCTACAN NNNNNNAACA CTGNGAAAAT CTTTGGTGTG GCTATTTACA CGGGAATGGA
  121 AACCAAGATG GCATTAAATT ATCAATCAAA ATCTCAGAAG CGATCTGCCG TGGAAAAATC
  181 GATGAATGCG TTCCTCATTG TGTATCTCTG CATTCTGATC AGCAAAGCCC TGATAAACAC
  241 TGTGCTGAAA TACGTGTGGC AGAGTGAGCC CTTTCGGGAT GAGCCGTGGT ATAATCAGAA
  301 AACGGAGTCG GAAAGGCAGA GGAATCTGTT CCTCAAGGCA TTCACGGACT TCCTGGCCTT
  361 CATGGTCCTC TTTAACTACA TCATCCCTGT GTCCATGTAC GTCACGGTCG AGATGCAGAA
  421 GTTCCTCGGC TCTTACTTCA TCACCTGGGA CGAAGACATG TTTGACGAGG AGACTGGCGA
  481 GGGGCCTCTG GTGAACACGT CGGACCTCAA TGAAGAGCTG GGACAGGTGG AGTACATCTT
  541 CACAGACAAG ACCGGCACCC TCACGGAAAA CAACATGGAG TTCAAGGAGT GCTGCATCGA
  601 AGGCCATGTC TACGTGCCCC ACGTCATCTG CAACGGGCAG GTCCTCCCAG AGTCGTCAGG
  661 AATCGACATG ATTGACTCGT CCCCCAGCGT CAACGGGAGG GAGCGCGAGG AGCTGTTTTT
  721 CCGGGCCCTC TGTCTCTGCC ACACCGTCCA GGTGAAAGAC GATGACAGCG TANACGGNCC
  781 CAGGAAATCG CCGGACGGGG GGAAATCCTG TGTGTACATC TCATCCTCGC CCGACNANGT
  841 GGCGCTGGTC GAANGTGTCC AGAGACTTGG CTTTACCTAC CTAAGGCTGA NNGANAATTA
  901 CNTGGAGATA TTAANCNGGG ANNANNCACA TCGAAAGGNT TGAATTGCTG GAAANTTTGA
  961 GTTTTGANNC NNNNNNANNN AGAATGANTG TANTTGNAAA TCTGCTNNNN GANAAANTTA
1021 TCTGTTTTGC NNNNGANNNN NNTCNNNNNN NTNCCCCCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Segawa, K., Human Type IV P-type ATPases That Work as Plasma Membrane Phospholipid Flippases and Their Regulation by Caspase and Calcium. J. Biol. Chem. 291 (2): 762-772 (2016). PMID 26567335. [link to RRC of NBRP]
original Segawa, K., Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure. Science 344 (6188): 1164-1168 (2014). PMID 24904167. [link to RRC of NBRP]