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pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: V5-GFP1-10; location: endoplasmic reticulum.

Catalog number RDB18663
Resource name pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10
Alternative name pMM86, pcDNA3.1-ERj1N-V5-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human DnaJ Heat Shock Protein Family (Hsp40) Member C1 (ERj1) N-terminal 200 a.a fused with V5-GFP1-10 at C-terminus.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human DNAJC1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
human DNAJC1 Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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          RDB18663 pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18663).

          Reference section:

          Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18663_B0Lnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, insert 5' (hERj1 (1-200), V5 tag)
          Sequence file: RDB18663_B0Lna.seq check
          >D07536A9-B0Ln_1_CMV_Forward_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TGNNGNNNNN GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
            121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA ACTTAAGCTT GGTACCGAGC
            181 TCGGATCCGC CACCATGACT GCTCCGTGCT CTCAACCCGC ACAACTGCCA GGACGGAGAC
            241 AGCTGGGTCT GGTACCTTTT CCTCCTCCAC CACCACGTAC ACCTCTGCTG TGGTTGCTGC
            301 TTCTGCTGCT TGCTGCGGTT GCACCGGCTA GAGGATGGGA ATCCGGCGAT TTGGAGCTGT
            361 TTGACCTGGT CGAAGAAGTG CAGTTGAACT TCTACCAGTT TCTCGGGGTA CAACAGGATG
            421 CCAGCTCAGC TGATATCCGC AAAGCCTATC GGAAATTGTC CCTCACACTG CATCCCGATA
            481 AGAACAAAGA CGAGAATGCC GAAACCCAGT TCAGACAGCT CGTGGCCATT TACGAGGTCC
            541 TGAAAGACGA TGAGAGGAGA CAGAGGTATG ACGACATTCT GATCAACGGA CTGCCCGACT
            601 GGCGACAGCC TGTGTTCTAC TATAGGCGAG TGCGGAAGAT GTCCAATGCC GAGCTTGCCC
            661 TGCTTCTCTT CATCATTCTC ACGGTTGGCC ACTATGCAGT CGTGTGGAGC ATCTACCTGG
            721 AGAAACAGCT GGATGAGCTC CTGTCACGCA AGAAGCGCGA AAAGAAGAAG AAAACCGGCA
            781 GTAAGAGCGT GGATGGGAAA CCCATACCCA ATCCACTCCT GGGTCTTGAC TCTACTGGAN
            841 GGAGTGGCGG CGCGGCCGCC GGTGGCACTA GTATGAGCAA AGGANAANAN CTTTTCNCTG
            901 GAGTTGNCCN NATTCTTGNT GAATTAGATG GNGATGTTNN NGGGGCACAA ATTTTCTGTC
            961 NNANGAGANG GTGAANGTGA TGCTANNNTC NGAAAACTCA CNNTNNNNTT ATTTGNNCTA
          1021 CNNNNAAANN NNCNNNNNNN NNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGH pA, insert 3' (GFP 1-10)
          Sequence file: RDB18663_B0Lnb.seq check
          >D07536A9-B0Ln_1_bGH_rev3_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGNNGGNNN NNNACNGANG GCTGGCAACT AGAAGGCACA
             61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGATT ACTTTTCGTT GGGATCTTTC
            121 GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA
            181 ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGG AACCATCTTC AACGTTGTGG
            241 CGAACTGTGA AGTTAGCTTT GATTCCATTC TTTTGTTTGT CTGCCGTGAT GTATACATTG
            301 TGTGAGTTAA AGTTGTACTC GAGTTTGTGT CCGAGAATGT TTCCATCTTC TTTAAAATCA
            361 GTACCCTTTA ACTCGATACG ATTAACAAGG GTATCACCTT CAAACTTGAC TACAGCACGC
            421 GTCTTGTATT TCCCGTCATC TTTGAAAGAT ATAGTGCGTT CCTGTACATA ACCTTCGGGC
            481 ATGGCACTCT TGAAAAAGTC ATGCCTTTTC ATGTGATCCG GATAACGGGA AAAGCATTGA
            541 ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT TCCAGTAGTG
            601 CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC TCCTCTGACA
            661 GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG
            721 AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCNGCGG NCGCGCCGCC ACTCCCTCCA
            781 GTAGAGTCAA GANCCANGAN TGGATTGGGT ATGGGTTTCC CATCCNCGCT CTTACTGCCG
            841 GTTTTCTTCT TCTTTTCGCG CTTCTTGCGN GANAGGANCT CATCCAGCTG NTTCTCCNNG
            901 NANANGCTCC ACACNANTGC NTANNGGNNA ACCGNGAGAA TGANGAANAG AANCNNNNCN
            961 ANGNNCNGNN TTNGNNNNTC TTNCNCACNN NNNNANNNTA NAANNCNNGN TGNNGNCNAN
          1021 NNNGNNNNNN NNNN
          //
          Primer: paGFP-M_R1 (Pr0893)
          Region: insert mid (GFP1-10 5', V5 tag, hERj1 (1-200) 3')
          Sequence file: RDB18663_B0Lnc.seq check
          >D07536A9-B0Ln_1_paGFP-M_R1_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNTTTTN NTGTGNNCCG GNNAACGGGA AAAGCATTGA
             61 ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT TCCAGTAGTG
            121 CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC TCCTCTGACA
            181 GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG
            241 AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCGGCGG CCGCGCCGCC ACTCCCTCCA
            301 GTAGAGTCAA GACCCAGGAG TGGATTGGGT ATGGGTTTCC CATCCACGCT CTTACTGCCG
            361 GTTTTCTTCT TCTTTTCGCG CTTCTTGCGT GACAGGAGCT CATCCAGCTG TTTCTCCAGG
            421 TAGATGCTCC ACACGACTGC ATAGTGGCCA ACCGTGAGAA TGATGAAGAG AAGCAGGGCA
            481 AGCTCGGCAT TGGACATCTT CCGCACTCGC CTATAGTAGA ACACAGGCTG TCGCCAGTCG
            541 GGCAGTCCGT TGATCAGAAT GTCGTCATAC CTCTGTCTCC TCTCATCGTC TTTCAGGACC
            601 TCGTAAATGG CCACGAGCTG TCTGAACTGG GTTTCGGCAT TCTCGTCTTT GTTCTTATCG
            661 GGATGCAGTG TGAGGGACAA TTTCCGATAG GCTTTGCGGA TATCAGCTGA NCTGGCATCC
            721 TGTTGTACCC CGAGAAACTG GTAGAAGTTC AACTGCACTT CTTCGACCAG GTCAAACAGC
            781 TCCNAAATCG CCGGATTCCC ATCCTCTAGC CGGTGCAACC GCAGCAAGCA GCAGAANNCA
            841 GCAACCACAG CAGANGTGTA CGTGGTGGNG GAGGAGGAAA AGGNACCAGA NCNAGCTGTC
            901 TNCNNCCNGG NCANTNGTGC GGGTTGANAG CANGGAGNAN TCNTGGNGGN NNGATNCNAN
            961 CTCGGTACNN AGCTNAAGTT TAAACNCTAN CCNGCTTNNN CTCCCNNNTA NTGAGTNGNA
          1021 NNNANTTTCN NNNAANNCNA GNAANNNNNN GGGNNNNNNN NNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, NeoR
          Sequence file: RDB18663_B0Lnd.seq check
          >D07536A9-B0Ln_1_SV40pro_ori_F_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNCG CCTCTGCCTC TGANCNATTC NNGAAGTAGT GAGGAGGCTT
             61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
            121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
            181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
            241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
            301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
            361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
            421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
            481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
            541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
            601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
            661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA NCCATGGCGA
            721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
            781 CCGGCTGGGT GTGGCGGANC GCTATCAGGA CATAGCGTTG GCTACCCGTG ANATTGCTGA
            841 AGAGCTTGGC GGCGAATGGG CTGANCGCTT CCTCNNGCTT TACNGNNTCG CCGCNNCCGA
            901 TTCNCANCGC ATCGCCTTCT ATCGCCTNCN TGANNAGTTC TTCTGANCGG GANTCTGGGG
          961 NTCNAAATGA CCGACCNNGC GACNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl