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pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10

The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human ERj1 N-terminal 200 a.a fused with V5-GFP1-10 at C-terminus.

Catalog number RDB18663
Resource name pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human DnaJ Heat Shock Protein Family (Hsp40) Member C1 (ERj1) N-terminal 200 a.a fused with V5-GFP1-10 at C-terminus.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human DNAJC1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank human DNAJC1 (NCBI Gene 64215) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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Schedule A (GFP-A) [Link]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18663 pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18663_B0Lnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5' (hERj1 (1-200), V5 tag)
Sequence file: RDB18663_B0Lna.seq check
>D07536A9-B0Ln_1_CMV_Forward_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TGNNGNNNNN GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA ACTTAAGCTT GGTACCGAGC
  181 TCGGATCCGC CACCATGACT GCTCCGTGCT CTCAACCCGC ACAACTGCCA GGACGGAGAC
  241 AGCTGGGTCT GGTACCTTTT CCTCCTCCAC CACCACGTAC ACCTCTGCTG TGGTTGCTGC
  301 TTCTGCTGCT TGCTGCGGTT GCACCGGCTA GAGGATGGGA ATCCGGCGAT TTGGAGCTGT
  361 TTGACCTGGT CGAAGAAGTG CAGTTGAACT TCTACCAGTT TCTCGGGGTA CAACAGGATG
  421 CCAGCTCAGC TGATATCCGC AAAGCCTATC GGAAATTGTC CCTCACACTG CATCCCGATA
  481 AGAACAAAGA CGAGAATGCC GAAACCCAGT TCAGACAGCT CGTGGCCATT TACGAGGTCC
  541 TGAAAGACGA TGAGAGGAGA CAGAGGTATG ACGACATTCT GATCAACGGA CTGCCCGACT
  601 GGCGACAGCC TGTGTTCTAC TATAGGCGAG TGCGGAAGAT GTCCAATGCC GAGCTTGCCC
  661 TGCTTCTCTT CATCATTCTC ACGGTTGGCC ACTATGCAGT CGTGTGGAGC ATCTACCTGG
  721 AGAAACAGCT GGATGAGCTC CTGTCACGCA AGAAGCGCGA AAAGAAGAAG AAAACCGGCA
  781 GTAAGAGCGT GGATGGGAAA CCCATACCCA ATCCACTCCT GGGTCTTGAC TCTACTGGAN
  841 GGAGTGGCGG CGCGGCCGCC GGTGGCACTA GTATGAGCAA AGGANAANAN CTTTTCNCTG
  901 GAGTTGNCCN NATTCTTGNT GAATTAGATG GNGATGTTNN NGGGGCACAA ATTTTCTGTC
  961 NNANGAGANG GTGAANGTGA TGCTANNNTC NGAAAACTCA CNNTNNNNTT ATTTGNNCTA
1021 CNNNNAAANN NNCNNNNNNN NNN
//
Primer: bGH_rev3
Region: bGH pA, insert 3' (GFP 1-10)
Sequence file: RDB18663_B0Lnb.seq check
>D07536A9-B0Ln_1_bGH_rev3_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNGGNNN NNNACNGANG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGATT ACTTTTCGTT GGGATCTTTC
  121 GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA
  181 ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGG AACCATCTTC AACGTTGTGG
  241 CGAACTGTGA AGTTAGCTTT GATTCCATTC TTTTGTTTGT CTGCCGTGAT GTATACATTG
  301 TGTGAGTTAA AGTTGTACTC GAGTTTGTGT CCGAGAATGT TTCCATCTTC TTTAAAATCA
  361 GTACCCTTTA ACTCGATACG ATTAACAAGG GTATCACCTT CAAACTTGAC TACAGCACGC
  421 GTCTTGTATT TCCCGTCATC TTTGAAAGAT ATAGTGCGTT CCTGTACATA ACCTTCGGGC
  481 ATGGCACTCT TGAAAAAGTC ATGCCTTTTC ATGTGATCCG GATAACGGGA AAAGCATTGA
  541 ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT TCCAGTAGTG
  601 CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC TCCTCTGACA
  661 GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG
  721 AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCNGCGG NCGCGCCGCC ACTCCCTCCA
  781 GTAGAGTCAA GANCCANGAN TGGATTGGGT ATGGGTTTCC CATCCNCGCT CTTACTGCCG
  841 GTTTTCTTCT TCTTTTCGCG CTTCTTGCGN GANAGGANCT CATCCAGCTG NTTCTCCNNG
  901 NANANGCTCC ACACNANTGC NTANNGGNNA ACCGNGAGAA TGANGAANAG AANCNNNNCN
  961 ANGNNCNGNN TTNGNNNNTC TTNCNCACNN NNNNANNNTA NAANNCNNGN TGNNGNCNAN
1021 NNNGNNNNNN NNNN
//
Primer: paGFP-M_R1
Region: insert mid (GFP1-10 5', V5 tag, hERj1 (1-200) 3')
Sequence file: RDB18663_B0Lnc.seq check
>D07536A9-B0Ln_1_paGFP-M_R1_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTTTTN NTGTGNNCCG GNNAACGGGA AAAGCATTGA
   61 ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT TCCAGTAGTG
  121 CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC TCCTCTGACA
  181 GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG
  241 AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCGGCGG CCGCGCCGCC ACTCCCTCCA
  301 GTAGAGTCAA GACCCAGGAG TGGATTGGGT ATGGGTTTCC CATCCACGCT CTTACTGCCG
  361 GTTTTCTTCT TCTTTTCGCG CTTCTTGCGT GACAGGAGCT CATCCAGCTG TTTCTCCAGG
  421 TAGATGCTCC ACACGACTGC ATAGTGGCCA ACCGTGAGAA TGATGAAGAG AAGCAGGGCA
  481 AGCTCGGCAT TGGACATCTT CCGCACTCGC CTATAGTAGA ACACAGGCTG TCGCCAGTCG
  541 GGCAGTCCGT TGATCAGAAT GTCGTCATAC CTCTGTCTCC TCTCATCGTC TTTCAGGACC
  601 TCGTAAATGG CCACGAGCTG TCTGAACTGG GTTTCGGCAT TCTCGTCTTT GTTCTTATCG
  661 GGATGCAGTG TGAGGGACAA TTTCCGATAG GCTTTGCGGA TATCAGCTGA NCTGGCATCC
  721 TGTTGTACCC CGAGAAACTG GTAGAAGTTC AACTGCACTT CTTCGACCAG GTCAAACAGC
  781 TCCNAAATCG CCGGATTCCC ATCCTCTAGC CGGTGCAACC GCAGCAAGCA GCAGAANNCA
  841 GCAACCACAG CAGANGTGTA CGTGGTGGNG GAGGAGGAAA AGGNACCAGA NCNAGCTGTC
  901 TNCNNCCNGG NCANTNGTGC GGGTTGANAG CANGGAGNAN TCNTGGNGGN NNGATNCNAN
  961 CTCGGTACNN AGCTNAAGTT TAAACNCTAN CCNGCTTNNN CTCCCNNNTA NTGAGTNGNA
1021 NNNANTTTCN NNNAANNCNA GNAANNNNNN GGGNNNNNNN NNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, NeoR
Sequence file: RDB18663_B0Lnd.seq check
>D07536A9-B0Ln_1_SV40pro_ori_F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNCG CCTCTGCCTC TGANCNATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
  181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
  241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
  301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
  361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
  421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
  481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
  541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
  601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
  661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA NCCATGGCGA
  721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
  781 CCGGCTGGGT GTGGCGGANC GCTATCAGGA CATAGCGTTG GCTACCCGTG ANATTGCTGA
  841 AGAGCTTGGC GGCGAATGGG CTGANCGCTT CCTCNNGCTT TACNGNNTCG CCGCNNCCGA
  901 TTCNCANCGC ATCGCCTTCT ATCGCCTNCN TGANNAGTTC TTCTGANCGG GANTCTGGGG
961 NTCNAAATGA CCGACCNNGC GACNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

2021.06.24

GNP_filter3_RDBDEP_html_210618.pl