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pYC141 pBS-GPDp-Ifa38-GFP1-10-CyC1ter-hphMX

The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae Ifa38 fused with GFP1-10 at C-terminus.

Catalog number RDB18659
Resource name pYC141 pBS-GPDp-Ifa38-GFP1-10-CyC1ter-hphMX
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, hphMX (hygromycin resistant marker).
Vector backbone pBlueScript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin (E. coli), Hygromycin B (yeast).
Gene/insert name S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae IFA38 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Schedule A (GFP-A) [Link]
          Remarks
          Please use Schedule A (GFP-A) additionally.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          Schedule A(GFP-A, 英語版のみ) [Link]
          備考
          Schedule A(GFP-A, 英語版のみ)が追加で必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18659 pYC141 pBS-GPDp-Ifa38-GFP1-10-CyC1ter-hphMX DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB18659zzt01.seq compiled by us

          Original reference

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18659_B0Lnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: f1ori_F2
          Region: f1 ori, T7 pro, insert 5' (GPD pro)
          Sequence file: RDB18659_B0Lna.seq check
          >D07536A5_B0Ln_2_f1ori_F2_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNTTTG NCGANTTCGN NCTATTGGTT AAAAAAATGA GCTGATTTAA
             61 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTCCAT TCGCCATTCA
            121 GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG
            181 CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC
            241 GACGTTGTAA AACGACGGCC AGTGAGCGCG CGTAATACGA CTCACTATAG GGCGAATTGG
            301 GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTAGTTTATC
            361 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA
            421 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA
            481 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG
            541 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC
            601 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG
            661 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT
            721 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA
            781 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAA
            841 GGNTGAAACC AGTTCCCTGA AATTATTCCN CTACTTGNCT AATAAGTATA TAAAGANGGN
            901 ANGTATTGAT NGTAANTCTG NAAATCTATT TCTTNAACTN NNNAAANTCN ACTTTNNNAG
          961 TTAGNCTTTT TTTTTANTTT TNAAANNNNN GANNNTNANT TNNNANNNNN NNNNN
          //
          Primer: pUC-CAPbs_up_F2
          Region: T3 pro, insert 3' (TEF pro, HygR 5')
          Sequence file: RDB18659_B0Lnb.seq check
          >D07536A5_B0Ln_2_pUC-CAPbs_up_F2_G10_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGCTTNNNN CTTNATGCTT CCGGCTCGTA TGTTGTGTGG
             61 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCG
            121 CGCAATTAAC CCTCACTAAA GGGAACAAAA GCTGGAGCTC CACCGCGGTG GCGGCCGCTC
            181 TAGAACTAGT GGATCCAGTC TTGACGTGCG CAGCTCAGGG GCATGATGTG ACTGTCGCCC
            241 GTACATTTAG CCCATACATC CCCATGTATA ATCATTTGCA TCCATACATT TTGATGGCCG
            301 CACGGCGCGA AGCAAAAATT ACGGCTCCTC GCTGCGGACC TGCGAGCAGG GAAACGCTCC
            361 CCTCACAGAC GCGTTGAATT GTCCCCACGC CGCGCCCCTG TAGAGAAATA TAAAAGGTTA
            421 GGATTTGCCA CTGAGGTTCT TCTTTCATAT ACTTCCTTTT AAAATCTTGC TAGGATACAG
            481 TTCTCACATC ACATCCGAAC ATAAACAACC ATGGGTAAAA AGCCTGAACT CACCGCGACG
            541 TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG
            601 GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG
            661 GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG
            721 GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT TCAGCGAGAG CCTGACCTAT
            781 TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGANC TGCCTGAAAC CGAACTGCNN
            841 GCTGTTCTGC AGCCGGNCGC GGANGCCATG GATGCGATCG CTGCGGNCGA TCTTANCCNG
            901 ANGANCGGGT NCGGNCNNTT NNNANCGCNN GGAATCGGNC AATACACTAC NTGGCGNGNN
            961 TTNNTATGNN CNANTGNTGN NNCCCATGNG NATNANNGGN NANCNNNNNN GGANNNNNNC
          1021 NNNNNNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Ifa38 5')
          Sequence file: RDB18659_B0Lnc.seq check
          >D07536A5_B0Ln_2_TDH3pro_F_F11_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNTGNAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT
             61 CTACTTTTAT AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT
            121 GCGGCCGCAT GACTTTTATG CAACAGCTTC AAGAGGCTGG GGAAAGATTT AGGTGTATCA
            181 ATGGTCTTTT ATGGGTTGTT TTTGGGCTTG GTGTCTTAAA ATGTACAACG TTATCACTTA
            241 GATTTTTAGC TCTTATTTTT GATCTTTTTT TACTACCAGC AGTCAATTTC GACAAGTATG
            301 GTGCTAAAAC TGGTAAATAC TGTGCTATCA CTGGTGCAAG TGACGGTATT GGTAAAGAAT
            361 TTGCTAGACA AATGGCGAAA CGTGGCTTCA ACTTGGTATT GATCTCGAGA ACGCAATCTA
            421 AATTGGAGGC TTTACAAAAA GAACTAGAAG ATCAACATCA TGTCGTTGTA AAGATTCTAG
            481 CCATTGACAT TGCGGAGGAT AAAGAATCCA ACTATGAATC CATTAAGGAA TTGTGTGCGC
            541 AGTTACCAAT CACCGTTTTG GTCAATAATG TTGGTCAATC ACACTCCATT CCTGTTCCAT
            601 TTTTGGAAAC AGAAGAGAAG GAGCTTAGAA ATATTATCAC TATCAATAAC ACTGCTACAT
            661 TATTAATTAC ACAAATCATT GCACCAAAGA TTGTGGAAAC TGTGAAAGCT GAAAACAAGA
            721 AGTCGGGTAC TCGTGGTTTG ATCTTAACCA TGGGCTCATT TGGTGGTCTG ATTCCCACCC
            781 CACTTTTGGC TACATACAGT GGTTCGAAAT CATTCTTACA AGGTTGGTCT AACTCTTTGG
            841 CTGGNGAATT ATCTAAAGAT GCTATCGATG TTGAATTAAT CATTTCATAT TTGGTCNCTA
            901 NCNCGATGNC TAAAATCNGA AGANCATCTT TNANGATCCN NAANCCACNN NANTTTGTAA
          961 AATCCNNTTT NANANNCGNT GGNNNANNNN NNNNNN
          //
          Primer: Ifa38_C-F
          Region: insert mid (Ifa38 3', GFP1-10 5')
          Sequence file: RDB18659_B0Lnd.seq check
          >D07536A5_B0Ln_2_Ifa38_C-F_H10_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCNGAGNNC TTTGGCGTTT ACTCTAANNN
             61 NNGTTAACTC CNTTAATTAT TCCTTCCNTA AATCTATCAG AATTAGAGCC TTAAAAAAAG
            121 CCGCAAGACA GGTTAAAAAG GAAGGATCCG GAGGGTCTGG TGGCGGATCA ATGGGTGGCA
            181 CTAGTATGAG CAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG
            241 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGAGGAGA GGGTGAAGGT GATGCTACAA
            301 TCGGAAAACT CACACTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGCCAA
            361 CACTTGTCAC TACTCTGACC TATGGTGTTC AATGCTTTTC CCGTTATCCG GATCACATGA
            421 AAAGGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA CGCACTATAT
            481 CTTTCAAAGA TGACGGGAAA TACAAGACGC GTGCTGTAGT CAAGTTTGAA GGTGATACCC
            541 TTGTTAATCG TATCGAGTTA AAGGGTACTG ATTTTAAAGA AGATGGAAAC ATTCTCGGAC
            601 ACAAACTCGA GTACAACTTT AACTCACACA ATGTATACAT CACGGCAGAC AAACAAAAGA
            661 ATGGAATCAA AGCTAACTTC ACAGTTCGCC ACAACGTTGA AGATGGTTCC GTTCNANCTA
            721 GCAGACCATT ATCAACAAAA TACTCCAATT GGCGATGGCC CTGTCCTTTT ACCAGACAAC
            781 CNTTACCTGT CGACNCNAAA CTGTCCTTTC GAAAGATCCC AACGAAAAGT NANNANNNNN
            841 ATATCAAGCT TATCGATACC GTCNANNTCN ANTCATGNNA NNNANNNNNN NTCNNNGCTN
            901 ACNTNNACNC CCTCCCCCCC NCATCCNCTC TNNNCGAAAA AGGNANNGAN NTNNNNNNAC
            961 CTGNAAGTCT ANGTCCNNNT TTATTTTTTA NANNNNNNGT NNNNNNNNAG ANNNNNTATT
          1021 NNNATTTNNA ANTTNNNNTT TTTTTNNNNN NNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP1-10 3')
          Sequence file: RDB18659_B0Lne.seq check
          >D07536A5_B0Ln_2_CYC1ter_R_G11_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN AGCGGNNGTG GGGGGNNGGC GTGAATGTAA GCGTGACATA
             61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTACTTTT
            121 CGTTGGGATC TTTCGAAAGG ACAGTTTGTG TCGACAGGTA ATGGTTGTCT GGTAAAAGGA
            181 CAGGGCCATC GCCAATTGGA GTATTTTGTT GATAATGGTC TGCTAGTTGA ACGGAACCAT
            241 CTTCAACGTT GTGGCGAACT GTGAAGTTAG CTTTGATTCC ATTCTTTTGT TTGTCTGCCG
            301 TGATGTATAC ATTGTGTGAG TTAAAGTTGT ACTCGAGTTT GTGTCCGAGA ATGTTTCCAT
            361 CTTCTTTAAA ATCAGTACCC TTTAACTCGA TACGATTAAC AAGGGTATCA CCTTCAAACT
            421 TGACTACAGC ACGCGTCTTG TATTTCCCGT CATCTTTGAA AGATATAGTG CGTTCCTGTA
            481 CATAACCTTC GGGCATGGCA CTCTTGAAAA AGTCATGCCT TTTCATGTGA TCCGGATAAC
            541 GGGAAAAGCA TTGAACACCA TAGGTCAGAG TAGTGACAAG TGTTGGCCAT GGAACAGGTA
            601 GTTTTCCAGT AGTGCAAATA AATTTAAGTG TGAGTTTTCC GANTGTAGCA TCACCTTCAC
            661 CCTCTCCTCT GACAGAAAAT TTGTGNCCAT TAACATCACC ATCTAATTCN ACAAGAATTG
            721 GGNACAACTC CNGTGAAAAG TTCTTCTCCT TTGCTCNTAC TANTGCCACC CATTGATCCN
            781 CCACCNGANC CTCCGGANCC TTCCTTTTTN ACCTGTCTTG CGGNTTTTTT TANNNTCTNN
            841 TTCTGANANA TTTATGGNAN GGAANNATTN NNGNANNTNA NNNTCTNANA NNAAANGCCN
          901 NNGGTCTCTG NAANTNNNAN NTGANANNNN NNNNGNNNCC NNNNNNGNNN NNNNNNN
          //
          Primer: CYC1ter_F
          Region: insert mid(HygR)
          Sequence file: RDB18659_B0Lnf.seq check
          >D07536A5_B0Ln_2_CYC1ter_F_H11_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NGCTCNNNNN NTNNNNNNTG NNGGCCGGGC AGATGATGTC
             61 GAGGCGAAAA AAAATATANN TCACGCTAAC ATTTGATTAA AATAGAACAA CTACAATATA
            121 AAAAAACTAT ACAAATGACA AGTTCTTGAA AACAAGAATC TTTTTATTGT CAGTACTGAT
            181 TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC GAGTACTTCT
            241 ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG CCCGACAGTC
            301 CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA
            361 TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG
            421 AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT CTGCTGCTCC
            481 ATACAAGCCA ACCACGGCCT CCAGAAGAAG ATGTTGGCGA CCTCGTATTG GGAATCCCCG
            541 AACATCGCCT CGCTCCAGTC AATGACCGCT GTTATGCGGC CATTGTCCGT CAGGACATTG
            601 TTGGAGCCGA AATCCGCGTG CACGAGGTGC CGGACTTCGG GGCAGTCCTC GGCCCAAAGC
            661 ATCAGCTCAT CGAGAGCCTG CGCGACGGAC GCACTGACGG TGTCGTCCAT CACAGTTTGC
            721 CAGTGATACA CATGGGGATC AGCAATCGCG CATATGAAAT CACGCCATGT AGTGTATTGA
            781 CCGATTCCTT GCGGTCCGAA TGGGNCGAAC CCGCTCGTCT GGCTAANATC GGNCGCANCG
            841 ATCGCATCCA TGGCCTCCGC GACCGGCTGC AGAACAGNNG GCAGTNCNGT TNCNNGCNNN
            901 CTTGCAACGN GACNCCCTGN GCNNGGNGGG AGANGCAATN NNCANGNTCT NGCTNAANNT
            961 CCCCNANGNC NAGCACTTCC GGAATCNGGN NCGCGGNCNN ANNCAAANNG NNNNNAAACN
          1021 TANNNATNNT NGNANNNNNC NNNNNGNNNN NNNNNN
          //
          Primer: GFP-C
          Region: insert mid(CYC1 ter,Hygr 3')
          Sequence file: RDB18659_B0Lng.seq check
          >D07536A5_B0Ln_2_GFP-C_F04_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTGTCCTTTC NANNNANCCC AACGAANANN
             61 ANGAATTCGA TATCAAGCTT ATCGATACCG TCGACCTCGA GTCATGTAAT TAGTTATGTC
            121 ACGCTTACAT TCACGCCCTC CCCCCACATC CGCTCTAACC GAAAAGGAAG GAGTTAGACA
            181 ACCTGAAGTC TAGGTCCCTA TTTATTTTTT TATAGTTATG TTAGTATTAA GAACGTTATT
            241 TATATTTCAA ATTTTTCTTT TTTTTCTGTA CAGACGCGTG TACGCATGTA ACATTATACT
            301 GAAAACCTTG CTTGAGAAGG TTTTGGGACG CTCGAAGGCT TTAATTTGCG GCCGGGCAGA
            361 TGATGTCGAG GCGAAAAAAA ATATAAATCA CGCTAACATT TGATTAAAAT AGAACAACTA
            421 CAATATAAAA AAACTATACA AATGACAAGT TCTTGAAAAC AAGAATCTTT TTATTGTCAG
            481 TACTGATTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC TATCGGCGAG
            541 TACTTCTACA CAGCCATCGG TCCAGACGGC CGCGCTTCTG CGGGCGATTT GTGTACGCCC
            601 GACAGTCCCG GCTCCGGATC GGACGATTGC GTCGCATCGA CCCTGCGCCC AAGCTGCATC
            661 ATCGAAATTG CCGTCAACCA AGCTCTGATA GAGTTGGTCA AGACCNATGC GGAGCATATA
            721 CGCCCGGAGC CGCGGCGATC CTGCAAGCTC CGGATGCCTC CNCTCGAAGT ANCGCGTCTG
            781 CTGCTCCATA CAAGCCNNCC ACGGNCTCCA GAAGAAGANG NTGGCGANCT CNTATTGGGA
            841 ATCCCCGAAC ATCGCCTCNC TNNAGTCAAT GACCGCNGTN NTGCGGCCAT TGTCCGTCAG
            901 GACATTGTTG GANCCNAAAT CCNCGNGNNC GANGNGNNNG NANTTCNGGG CNNTCNTCNN
            961 NCCNNANNNN NNNNNTCATN NANAGNCTGN GCNANNNANG CACNGNNNGT GNNNTCCNNC
          1021 ANAGNTTNGN CAGNGNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          2022.04.07

          GNP_filter3_RDBDEP_html_220305.pl